Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DTQ05_RS11565 Genome accession   NZ_CP030931
Coordinates   2214733..2215779 (-) Length   348 a.a.
NCBI ID   WP_149427679.1    Uniprot ID   -
Organism   Exiguobacterium acetylicum strain AMCC 101217     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2209733..2220779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DTQ05_RS11545 - 2210284..2212023 (-) 1740 WP_149427675.1 fimbrial assembly protein -
  DTQ05_RS11550 - 2212042..2212797 (-) 756 WP_149427676.1 A24 family peptidase -
  DTQ05_RS11555 - 2212858..2213373 (-) 516 WP_149427677.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DTQ05_RS11560 - 2213511..2214728 (-) 1218 WP_149427678.1 type II secretion system F family protein -
  DTQ05_RS11565 pilT 2214733..2215779 (-) 1047 WP_149427679.1 type IV pilus twitching motility protein PilT Machinery gene
  DTQ05_RS11570 - 2215776..2217440 (-) 1665 WP_149427680.1 ATPase, T2SS/T4P/T4SS family -
  DTQ05_RS11575 - 2217449..2217763 (-) 315 WP_029342268.1 hypothetical protein -
  DTQ05_RS11580 - 2217779..2219101 (-) 1323 WP_244290048.1 G5 domain-containing protein -
  DTQ05_RS11585 - 2219331..2219858 (+) 528 WP_149427681.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DTQ05_RS11590 - 2219821..2220441 (+) 621 WP_029342271.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38170.87 Da        Isoelectric Point: 7.1317

>NTDB_id=302492 DTQ05_RS11565 WP_149427679.1 2214733..2215779(-) (pilT) [Exiguobacterium acetylicum strain AMCC 101217]
MNSTMERAQIEDILRSSKERGASDVHLTAGSPPVFRINGTLTPFGTEKMKPIIIDGFVQSLITEEMYQQLKQDRDIDFSF
GVTGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAASPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=302492 DTQ05_RS11565 WP_149427679.1 2214733..2215779(-) (pilT) [Exiguobacterium acetylicum strain AMCC 101217]
ATGAACAGTACGATGGAACGGGCACAAATCGAAGATATCTTACGCTCATCAAAAGAACGAGGAGCGTCCGATGTTCACTT
GACGGCGGGATCACCACCCGTCTTTCGAATCAACGGAACGTTGACACCGTTTGGGACAGAAAAGATGAAACCGATCATCA
TTGATGGATTTGTGCAGTCACTAATCACGGAAGAGATGTATCAACAACTGAAGCAGGACCGGGACATCGACTTTTCGTTC
GGTGTGACCGGTGTCGCTCGGTACCGAGTCAACTGTTTTTACCAACGGGGCGCTTTGTCGATGGCGTTTCGAACGATTCC
GACCGAGGTGCCAACGCTTGAGCAATTAGCGTTACCGCCTGTCTTAAAACGATTCTTAACGAAACCACACGGCTTGATTC
TCGTGACAGGTCCGACGGGTTCCGGGAAATCGACGACGCTTGCAGCGATGATCAATGAAATTAATCAGACGATGTACAAA
CGGATCATTACGCTGGAAGATCCGATTGAGTATTTACATAGTCATCATAAAAGCCTGATCGATCAGCGGGAAATCGGGAT
TGATGCGCCACGTTTCGCGACCGGGTTACGGTCAGCACTCCGTCAAGATCCGGATGTCATTTTGCTTGGAGAGATGCGGG
ACCTTGAAACGATCGCGACCGCCGTGACAGCAGCAGAGACAGGTCACCTCGTCTTTGCAACGGTCCATACGTCGACGGCT
GCCTCGACAGTCAGCCGAATCATCGATGTTTTCCCGTCAGAGCAACAAGATCAGATTCGGGCTCAACTGGCGAACGTTCT
TGCGGGCGTCGTCTCGCAACGCCTGATGCCACGTTCAGACGGCACAGGACGTGTCGCAGCTCTCGAGATCATGGTCGATA
CACCAGCTGTATCGAACTTGATTCGGACCGGTAAGGAATATCAGATTCAATCCGTCCTGCAGACGGGTAAACAATACGGC
ATGCAGACGATGCAGATGGCATTGCAGGATCTCGTCAGTCGGGGATTGATTCCGGCTTCTGCTGCATCACCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.253

0.486
  pilT Neisseria meningitidis 8013

50

95.977

0.48
  pilT Neisseria gonorrhoeae MS11

50

95.977

0.48
  pilT Acinetobacter nosocomialis M2

48.949

95.69

0.468
  pilT Pseudomonas aeruginosa PAK

48.949

95.69

0.468
  pilT Acinetobacter baylyi ADP1

47.941

97.701

0.468
  pilT Acinetobacter baumannii D1279779

48.649

95.69

0.466
  pilT Acinetobacter baumannii strain A118

48.649

95.69

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

94.828

0.466
  pilT Vibrio cholerae strain A1552

49.091

94.828

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.748

95.69

0.457
  pilT Legionella pneumophila strain Lp02

46.246

95.69

0.443
  pilT Legionella pneumophila strain ERS1305867

46.246

95.69

0.443
  pilU Acinetobacter baylyi ADP1

40.361

95.402

0.385
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.54

0.382

Multiple sequence alignment