Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AYN53_RS16885 Genome accession   NZ_CP030783
Coordinates   3305336..3306316 (-) Length   326 a.a.
NCBI ID   WP_113650379.1    Uniprot ID   -
Organism   Escherichia albertii strain 2012EL-1823B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3300336..3311316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYN53_RS16855 - 3300991..3301488 (+) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -
  AYN53_RS16860 endA 3301583..3302290 (+) 708 WP_002461298.1 deoxyribonuclease I -
  AYN53_RS16865 rsmE 3302370..3303101 (+) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AYN53_RS16870 gshB 3303114..3304064 (+) 951 WP_000593300.1 glutathione synthase -
  AYN53_RS16875 - 3304173..3304736 (+) 564 WP_113650378.1 YqgE/AlgH family protein -
  AYN53_RS16880 ruvX 3304736..3305152 (+) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  AYN53_RS16885 pilT 3305336..3306316 (-) 981 WP_113650379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AYN53_RS16890 yggS 3306334..3307038 (+) 705 WP_059220282.1 pyridoxal phosphate homeostasis protein -
  AYN53_RS16895 yggT 3307056..3307622 (+) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  AYN53_RS16900 yggU 3307619..3307909 (+) 291 WP_113650380.1 DUF167 family protein YggU -
  AYN53_RS16905 - 3307917..3308510 (+) 594 WP_113650381.1 XTP/dITP diphosphatase -
  AYN53_RS16910 hemW 3308503..3309639 (+) 1137 WP_059220280.1 radical SAM family heme chaperone HemW -
  AYN53_RS16915 - 3309704..3310711 (-) 1008 WP_113650382.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.15 Da        Isoelectric Point: 5.5884

>NTDB_id=301470 AYN53_RS16885 WP_113650379.1 3305336..3306316(-) (pilT) [Escherichia albertii strain 2012EL-1823B]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREEPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=301470 AYN53_RS16885 WP_113650379.1 3305336..3306316(-) (pilT) [Escherichia albertii strain 2012EL-1823B]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGAGCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393

Multiple sequence alignment