Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHD70_RS05685 Genome accession   NZ_CP030203
Coordinates   1100939..1101919 (+) Length   326 a.a.
NCBI ID   WP_114046776.1    Uniprot ID   -
Organism   Salmonella enterica strain SA20083530     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1095939..1106919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHD70_RS05655 (CHD70_05655) - 1096516..1097523 (+) 1008 WP_079962290.1 DUF1202 family protein -
  CHD70_RS05660 (CHD70_05660) hemW 1097615..1098751 (-) 1137 WP_114046775.1 radical SAM family heme chaperone HemW -
  CHD70_RS05665 (CHD70_05665) - 1098744..1099337 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  CHD70_RS05670 (CHD70_05670) yggU 1099345..1099635 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  CHD70_RS05675 (CHD70_05675) - 1099632..1100198 (-) 567 WP_001094848.1 YggT family protein -
  CHD70_RS05680 (CHD70_05680) - 1100217..1100921 (-) 705 WP_000997792.1 YggS family pyridoxal phosphate-dependent enzyme -
  CHD70_RS05685 (CHD70_05685) pilT 1100939..1101919 (+) 981 WP_114046776.1 type IV pilus twitching motility protein PilT Machinery gene
  CHD70_RS05690 (CHD70_05690) - 1102051..1102752 (+) 702 WP_023217481.1 IclR family transcriptional regulator -
  CHD70_RS05695 (CHD70_05695) ruvX 1102784..1103200 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  CHD70_RS05700 (CHD70_05700) - 1103200..1103763 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  CHD70_RS05705 (CHD70_05705) gshB 1103979..1104926 (-) 948 WP_000593248.1 glutathione synthase -
  CHD70_RS05710 (CHD70_05710) rsmE 1104946..1105677 (-) 732 WP_114047546.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CHD70_RS05715 (CHD70_05715) endA 1105754..1106461 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36152.45 Da        Isoelectric Point: 7.5114

>NTDB_id=300052 CHD70_RS05685 WP_114046776.1 1100939..1101919(+) (pilT) [Salmonella enterica strain SA20083530]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDKLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQMPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=300052 CHD70_RS05685 WP_114046776.1 1100939..1101919(+) (pilT) [Salmonella enterica strain SA20083530]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAAGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GATGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

45.283

97.546

0.442
  pilT Legionella pneumophila strain Lp02

45.283

97.546

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365

Multiple sequence alignment