Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DPF84_RS13500 Genome accession   NZ_CP030076
Coordinates   2707395..2708375 (+) Length   326 a.a.
NCBI ID   WP_058673063.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 20710     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2702395..2713375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPF84_RS13460 (DPF84_13455) - 2702425..2702751 (+) 327 WP_003862421.1 YggL family protein -
  DPF84_RS13465 (DPF84_13460) - 2702809..2703525 (+) 717 WP_003862419.1 DUF2884 domain-containing protein -
  DPF84_RS13470 (DPF84_13465) - 2703638..2704000 (-) 363 WP_045345612.1 DUF559 domain-containing protein -
  DPF84_RS13475 (DPF84_13470) hemW 2704057..2705205 (-) 1149 WP_045332868.1 radical SAM family heme chaperone HemW -
  DPF84_RS13480 (DPF84_13475) - 2705198..2705791 (-) 594 WP_022651831.1 XTP/dITP diphosphatase -
  DPF84_RS13485 (DPF84_13480) yggU 2705795..2706091 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  DPF84_RS13490 (DPF84_13485) - 2706088..2706654 (-) 567 WP_003860024.1 YggT family protein -
  DPF84_RS13495 (DPF84_13490) - 2706676..2707377 (-) 702 WP_045345615.1 YggS family pyridoxal phosphate-dependent enzyme -
  DPF84_RS13500 (DPF84_13495) pilT 2707395..2708375 (+) 981 WP_058673063.1 type IV pilus twitching motility protein PilT Machinery gene
  DPF84_RS13505 (DPF84_13500) ruvX 2708386..2708802 (-) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  DPF84_RS13510 (DPF84_13505) - 2708802..2709362 (-) 561 WP_003860031.1 YqgE/AlgH family protein -
  DPF84_RS13515 (DPF84_13510) gshB 2709437..2710384 (-) 948 WP_023323729.1 glutathione synthase -
  DPF84_RS13520 (DPF84_13515) rsmE 2710412..2711143 (-) 732 WP_045345616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DPF84_RS13525 (DPF84_13520) endA 2711195..2711902 (-) 708 WP_003860034.1 deoxyribonuclease I -
  DPF84_RS13530 (DPF84_13525) - 2711997..2712494 (-) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35767.78 Da        Isoelectric Point: 6.2344

>NTDB_id=298957 DPF84_RS13500 WP_058673063.1 2707395..2708375(+) (pilT) [Enterobacter hormaechei strain 20710]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLADRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSAMREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=298957 DPF84_RS13500 WP_058673063.1 2707395..2708375(+) (pilT) [Enterobacter hormaechei strain 20710]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCTCCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCCTGGTGGGCAAATGGGCAGGTTGATTTTGCCGTTACCCTCGCAGACCGTCAGCGCCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCGATGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAACTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377

Multiple sequence alignment