Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SCE_RS22920 Genome accession   NC_010162
Coordinates   6304955..6306094 (+) Length   379 a.a.
NCBI ID   WP_012237100.1    Uniprot ID   A9F877
Organism   Sorangium cellulosum So ce56     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6299955..6311094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCE_RS22900 - 6300378..6300566 (+) 189 WP_044965527.1 hypothetical protein -
  SCE_RS22905 (sce4465) - 6300581..6301924 (-) 1344 WP_044965530.1 serine/threonine-protein kinase -
  SCE_RS22910 (sce4466) - 6301921..6303144 (-) 1224 WP_012237098.1 FHA domain-containing protein -
  SCE_RS22915 (sce4467) pilB 6303250..6304950 (+) 1701 WP_012237099.1 type IV-A pilus assembly ATPase PilB -
  SCE_RS22920 (sce4468) pilT 6304955..6306094 (+) 1140 WP_012237100.1 type IV pilus twitching motility protein PilT Machinery gene
  SCE_RS22925 (sce4469) lexA 6306286..6306978 (+) 693 WP_012237101.1 transcriptional repressor LexA -
  SCE_RS22930 (sce4470) - 6307087..6307413 (+) 327 WP_012237102.1 DUF4398 domain-containing protein -
  SCE_RS22935 (sce4471) - 6307422..6308570 (+) 1149 WP_231865016.1 OmpA family protein -
  SCE_RS22940 (sce4472) - 6308839..6310476 (+) 1638 WP_231865017.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41850.35 Da        Isoelectric Point: 6.9233

>NTDB_id=29710 SCE_RS22920 WP_012237100.1 6304955..6306094(+) (pilT) [Sorangium cellulosum So ce56]
MSTELVGQQEGLRYTLQQLLRAMVEKGASDMHITSGTPPLLRIDGTVVPLKLPPLMPSDTKQLCYSVLSEEQVAAFEQRN
ELDLSFGMKGLARFRANIYMQRGAVAAAFRQIPFRIMNFDELGLPPVVLDITAKPRGLVLVTGPTGSGKTTTLASIIDKI
NSEQRLHILTIEDPIEYLHPHKLSLVNQREIGSDTASFKDALRYALRQDPDVVLVGEMRDLETVESALTIAETGHLVFAT
LHTNSAISTINRIIDVFPPHQQSQVRQQLSFTLVAVMTQLLLPRANGPGRVMAMEVMIPNAAIRNLIREDKLHQIYSQMQ
VGQAGSGMQTMAQSLVSLYQRRMITLEEAFAAAADGEEIRAMLEPRQAVSSRNSGPKQA

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=29710 SCE_RS22920 WP_012237100.1 6304955..6306094(+) (pilT) [Sorangium cellulosum So ce56]
ATGAGCACCGAGCTCGTCGGACAGCAGGAAGGCCTCCGGTACACGCTCCAGCAGCTCCTCCGCGCGATGGTGGAGAAGGG
CGCGAGCGACATGCACATCACGAGCGGCACGCCGCCGCTCCTGCGCATCGACGGCACGGTGGTGCCGCTGAAGCTCCCGC
CGCTCATGCCGAGCGACACGAAGCAGCTCTGCTACTCGGTGCTGAGCGAGGAGCAGGTGGCGGCGTTCGAGCAGCGCAAC
GAGCTCGATCTCTCGTTCGGCATGAAGGGGCTCGCCCGCTTCCGCGCCAACATCTACATGCAGCGCGGCGCCGTCGCGGC
CGCGTTCCGGCAGATCCCCTTCCGGATCATGAACTTCGACGAGCTGGGCCTGCCGCCGGTCGTGCTCGACATCACCGCCA
AGCCCCGCGGCCTCGTGCTCGTGACGGGCCCGACGGGCTCGGGCAAGACGACCACGCTCGCGAGCATCATCGACAAGATC
AACTCGGAGCAGCGGCTGCACATCCTCACGATCGAGGATCCGATCGAGTACCTGCACCCGCACAAGCTGTCGCTCGTGAA
CCAGCGCGAGATCGGCTCCGACACCGCGTCGTTCAAGGACGCCCTGCGGTACGCCCTGCGCCAGGATCCGGACGTCGTGC
TCGTCGGTGAGATGCGCGACCTCGAGACCGTCGAGTCGGCGCTGACGATCGCCGAGACCGGCCACCTCGTGTTCGCCACG
CTCCACACGAACTCGGCGATCTCGACGATCAACCGCATCATCGACGTCTTCCCGCCGCACCAGCAGTCGCAGGTGCGCCA
GCAGCTCTCGTTCACGCTCGTCGCCGTGATGACGCAGCTCCTCCTGCCGCGCGCGAACGGCCCGGGGCGCGTCATGGCGA
TGGAGGTCATGATCCCGAACGCGGCGATCCGGAACCTCATCCGCGAGGACAAGCTCCACCAGATCTACTCGCAGATGCAG
GTGGGCCAGGCCGGCAGCGGCATGCAGACGATGGCCCAGTCGCTCGTGTCCCTCTACCAGCGCCGGATGATCACGCTGGA
GGAGGCGTTCGCGGCGGCCGCCGACGGCGAAGAGATCCGGGCGATGCTGGAGCCCCGTCAGGCGGTGAGCTCACGGAACA
GCGGCCCGAAGCAGGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A9F877

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.523

90.765

0.504
  pilT Legionella pneumophila strain Lp02

52.959

89.182

0.472
  pilT Legionella pneumophila strain ERS1305867

52.959

89.182

0.472
  pilT Acinetobacter baylyi ADP1

51.17

90.237

0.462
  pilT Acinetobacter nosocomialis M2

51.183

89.182

0.456
  pilT Acinetobacter baumannii D1279779

51.183

89.182

0.456
  pilT Acinetobacter baumannii strain A118

51.183

89.182

0.456
  pilT Pseudomonas stutzeri DSM 10701

51.343

88.391

0.454
  pilT Pseudomonas aeruginosa PAK

50.746

88.391

0.449
  pilT Neisseria meningitidis 8013

49.128

90.765

0.446
  pilT Neisseria gonorrhoeae MS11

48.837

90.765

0.443
  pilT Vibrio cholerae strain A1552

49.55

87.863

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.55

87.863

0.435
  pilU Vibrio cholerae strain A1552

39.943

93.14

0.372
  pilU Pseudomonas stutzeri DSM 10701

40.115

92.084

0.369

Multiple sequence alignment