Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B1H56_RS00585 Genome accession   NZ_CP029256
Coordinates   118540..119595 (-) Length   351 a.a.
NCBI ID   WP_066522211.1    Uniprot ID   A0A136Q1X2
Organism   Christensenella minuta strain DSM 22607     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 113540..124595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B1H56_RS00550 (B1H56_00550) - 114797..115351 (-) 555 WP_066522222.1 TetR/AcrR family transcriptional regulator -
  B1H56_RS00555 (B1H56_00555) - 115466..116296 (+) 831 WP_162938942.1 MBL fold metallo-hydrolase -
  B1H56_RS00560 (B1H56_00560) rlmH 116329..116802 (+) 474 WP_066522221.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  B1H56_RS00565 - 116877..117014 (+) 138 WP_121418944.1 bacteriocin -
  B1H56_RS00570 (B1H56_00565) - 117190..117450 (+) 261 WP_121418945.1 transketolase C-terminal domain-containing protein -
  B1H56_RS14910 (B1H56_00570) - 117548..117964 (-) 417 WP_066522215.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B1H56_RS00580 (B1H56_00575) - 118143..118463 (-) 321 WP_066522214.1 hypothetical protein -
  B1H56_RS00585 (B1H56_00580) pilT 118540..119595 (-) 1056 WP_066522211.1 type IV pilus twitching motility protein PilT Machinery gene
  B1H56_RS00590 (B1H56_00585) - 119609..121315 (-) 1707 WP_066522209.1 GspE/PulE family protein -
  B1H56_RS00595 (B1H56_00590) - 121506..122270 (+) 765 WP_066522206.1 A24 family peptidase -
  B1H56_RS00600 (B1H56_00595) - 122274..122930 (-) 657 WP_066522199.1 hypothetical protein -
  B1H56_RS00605 (B1H56_00600) - 122927..123472 (-) 546 WP_162938943.1 hypothetical protein -
  B1H56_RS00610 (B1H56_00605) pilM 123499..124413 (-) 915 WP_162938944.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38478.23 Da        Isoelectric Point: 6.0678

>NTDB_id=291373 B1H56_RS00585 WP_066522211.1 118540..119595(-) (pilT) [Christensenella minuta strain DSM 22607]
MIDELIGMAREMDCSDIHIACGEPPIVRKNGELKRLSGRPVFTPQTVAEIAREMLQKSGGSGSPEDGDVDFSYQTPSGER
QRVNIFRQRGEIGIAVRLLQKDIPTIGELNLPSIFAEIADYPRGLVLVTGPTGSGKSTTLAAMIDAVNEKRSCHILTLED
PVEYMHASKKSMVNQREIGRDARSFSSALRSALREDPDVILVGEMRDLETISSAITAAETGHLVMSTLHTIGAAKTIDRI
IDVFPPYQQQQVRTQLATVLRAVITQTLIPRADGSGRVPAFEIMLVNDAVANLVREGKTFQIASVLQTNTRTGMICLDMY
LARLVKDGTITMEAALDRAASKEELKRYVQI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=291373 B1H56_RS00585 WP_066522211.1 118540..119595(-) (pilT) [Christensenella minuta strain DSM 22607]
ATGATAGACGAATTGATTGGCATGGCCCGCGAGATGGACTGTTCGGACATCCATATCGCCTGCGGCGAGCCACCGATCGT
GCGTAAAAACGGCGAATTGAAGCGACTTTCGGGCAGGCCCGTATTTACCCCGCAGACAGTTGCCGAAATCGCGCGGGAGA
TGCTGCAAAAAAGCGGCGGCAGCGGCAGCCCGGAGGACGGGGACGTCGATTTTTCGTACCAGACACCGTCCGGGGAGCGG
CAAAGGGTCAATATCTTCCGCCAGCGGGGCGAGATTGGCATCGCCGTCCGCCTGCTGCAAAAGGATATTCCGACCATCGG
CGAACTGAACCTGCCTTCCATCTTTGCGGAAATTGCGGATTACCCGCGCGGCCTTGTTCTGGTGACCGGGCCGACCGGAA
GCGGTAAATCCACCACGCTCGCGGCGATGATCGACGCGGTGAACGAAAAACGCAGCTGCCACATCCTGACGCTTGAGGAC
CCGGTAGAATATATGCACGCGAGCAAAAAGAGCATGGTCAACCAGCGCGAGATCGGGCGCGACGCAAGGAGCTTTTCTTC
CGCGCTCAGGAGCGCCCTGCGCGAGGACCCGGACGTGATCCTTGTGGGGGAAATGCGCGACCTCGAGACCATCAGCTCCG
CCATTACCGCGGCGGAGACCGGACACCTGGTGATGTCTACTCTGCATACCATCGGCGCGGCCAAGACCATCGACAGGATT
ATCGACGTGTTTCCGCCTTACCAGCAGCAACAGGTGCGCACGCAGCTGGCGACGGTCCTTAGGGCCGTCATCACGCAGAC
GCTGATCCCCCGCGCGGACGGTTCAGGACGCGTTCCGGCTTTTGAGATTATGCTGGTGAACGACGCTGTGGCAAACCTTG
TCCGCGAGGGCAAGACCTTCCAGATCGCCTCCGTATTGCAGACGAACACGCGGACGGGAATGATCTGCCTCGATATGTAT
CTTGCGCGGCTGGTGAAGGACGGTACAATTACTATGGAGGCTGCCCTCGACCGGGCGGCCAGCAAAGAGGAGTTGAAGCG
TTACGTCCAAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A136Q1X2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.734

98.575

0.51
  pilT Acinetobacter baylyi ADP1

50

98.006

0.49
  pilT Acinetobacter nosocomialis M2

49.708

97.436

0.484
  pilT Acinetobacter baumannii D1279779

49.708

97.436

0.484
  pilT Acinetobacter baumannii strain A118

49.708

97.436

0.484
  pilT Neisseria meningitidis 8013

48.986

98.291

0.481
  pilT Neisseria gonorrhoeae MS11

48.986

98.291

0.481
  pilT Pseudomonas aeruginosa PAK

49.128

98.006

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.76

93.732

0.476
  pilT Vibrio cholerae strain A1552

50.76

93.732

0.476
  pilT Pseudomonas stutzeri DSM 10701

47.674

98.006

0.467
  pilT Legionella pneumophila strain Lp02

47.801

97.151

0.464
  pilT Legionella pneumophila strain ERS1305867

47.801

97.151

0.464
  pilU Vibrio cholerae strain A1552

38.739

94.872

0.368
  pilU Pseudomonas stutzeri DSM 10701

36.857

99.715

0.368

Multiple sequence alignment