Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM410_RS11130 Genome accession   NZ_CP028950
Coordinates   2250760..2251785 (+) Length   341 a.a.
NCBI ID   WP_108647820.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. FDA-CDC-AR_0164     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2245760..2256785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM410_RS11090 (AM410_11080) - 2245841..2246167 (+) 327 WP_020883688.1 YggL family protein -
  AM410_RS11095 (AM410_11085) - 2246224..2246940 (+) 717 WP_020883689.1 DUF2884 domain-containing protein -
  AM410_RS11100 (AM410_11090) - 2247003..2247364 (-) 362 Protein_2104 endonuclease domain-containing protein -
  AM410_RS11105 (AM410_11095) hemW 2247428..2248570 (-) 1143 WP_108647818.1 radical SAM family heme chaperone HemW -
  AM410_RS11110 (AM410_11100) - 2248563..2249156 (-) 594 WP_014171572.1 XTP/dITP diphosphatase -
  AM410_RS11115 (AM410_11105) yggU 2249160..2249456 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  AM410_RS11120 (AM410_11110) - 2249453..2250019 (-) 567 WP_014171570.1 YggT family protein -
  AM410_RS11125 (AM410_11115) - 2250041..2250742 (-) 702 WP_044865459.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM410_RS11130 (AM410_11120) pilT 2250760..2251785 (+) 1026 WP_108647820.1 type IV pilus twitching motility protein PilT Machinery gene
  AM410_RS11135 (AM410_11125) ruvX 2251751..2252167 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  AM410_RS11140 (AM410_11130) - 2252167..2252727 (-) 561 WP_020883695.1 YqgE/AlgH family protein -
  AM410_RS11145 (AM410_11135) gshB 2252814..2253761 (-) 948 WP_020883696.1 glutathione synthase -
  AM410_RS11150 (AM410_11140) rsmE 2253801..2254532 (-) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM410_RS11155 (AM410_11145) endA 2254585..2255292 (-) 708 WP_106503478.1 deoxyribonuclease I -
  AM410_RS11160 (AM410_11150) - 2255387..2255884 (-) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37162.17 Da        Isoelectric Point: 6.0946

>NTDB_id=288864 AM410_RS11130 WP_108647820.1 2250760..2251785(+) (pilT) [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLSVLGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADAQRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=288864 AM410_RS11130 WP_108647820.1 2250760..2251785(+) (pilT) [Enterobacter cloacae complex sp. FDA-CDC-AR_0164]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCGCCCGAAGTCGATACGCTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTTACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGTGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCAGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.817

99.12

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

99.12

0.484
  pilT Neisseria meningitidis 8013

48.048

97.654

0.469
  pilT Neisseria gonorrhoeae MS11

46.491

100

0.466
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.401

95.894

0.455
  pilT Acinetobacter baylyi ADP1

46.687

97.361

0.455
  pilT Acinetobacter baumannii strain A118

47.095

95.894

0.452
  pilT Acinetobacter baumannii D1279779

47.095

95.894

0.452
  pilT Pseudomonas aeruginosa PAK

47.095

95.894

0.452
  pilT Acinetobacter nosocomialis M2

47.5

93.842

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

94.428

0.419
  pilU Pseudomonas stutzeri DSM 10701

40

96.774

0.387
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369

Multiple sequence alignment