Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHAB381_RS07670 Genome accession   NC_009714
Coordinates   1565458..1566543 (+) Length   361 a.a.
NCBI ID   WP_012109472.1    Uniprot ID   A7I3S5
Organism   Campylobacter hominis ATCC BAA-381     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1560458..1571543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHAB381_RS07640 (CHAB381_1637) hisG 1561919..1562527 (-) 609 WP_012109465.1 ATP phosphoribosyltransferase -
  CHAB381_RS07645 (CHAB381_1638) - 1562527..1563147 (-) 621 WP_012109466.1 type III pantothenate kinase -
  CHAB381_RS07650 (CHAB381_1639) - 1563134..1563466 (-) 333 WP_012109467.1 hypothetical protein -
  CHAB381_RS07655 (CHAB381_1640) - 1563456..1564472 (-) 1017 WP_012109468.1 hypothetical protein -
  CHAB381_RS07660 (CHAB381_1641) - 1564469..1565038 (-) 570 WP_041570537.1 hypothetical protein -
  CHAB381_RS07665 (CHAB381_1642) gatC 1565153..1565446 (+) 294 WP_012109471.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CHAB381_RS07670 (CHAB381_1643) pilT 1565458..1566543 (+) 1086 WP_012109472.1 type IV pilus twitching motility protein PilT Machinery gene
  CHAB381_RS07675 (CHAB381_1644) - 1566543..1567199 (+) 657 WP_012109473.1 CvpA family protein -
  CHAB381_RS07680 (CHAB381_1645) - 1567209..1567676 (+) 468 WP_012109474.1 Fur family transcriptional regulator -
  CHAB381_RS07685 (CHAB381_1646) lysS 1567676..1569175 (+) 1500 WP_012109475.1 lysine--tRNA ligase -
  CHAB381_RS07690 (CHAB381_1647) - 1569178..1570425 (+) 1248 WP_012109476.1 serine hydroxymethyltransferase -
  CHAB381_RS07695 (CHAB381_1648) - 1570511..1571452 (+) 942 WP_012109477.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 40330.23 Da        Isoelectric Point: 6.5074

>NTDB_id=28801 CHAB381_RS07670 WP_012109472.1 1565458..1566543(+) (pilT) [Campylobacter hominis ATCC BAA-381]
MVEENRKHLADIETLLKTVVFNKASDLHLVSRSAPQVRIDGKLRPLAMDVLTGSDIEYICYALITDAQKSELEENKELDF
AIELPNIGRFRGNYYYTMNGDLAAAFRIIPIDIPSLDELKAPSIFKEIVKHEKGMILVTGPTGSGKSTTLAAMLNEINET
ERKHIITVEDPVEFVHTNKRSLFSHRNIGTDTQSYARALKFSLREDPDIILVGELRDRETISIAITAAETGHLVFGTLHT
NSAIQSLNRIIDSFDGGEQLQVRNMLSVSLTSIISQSLLPRIGKGRVAIHEILINNNAIANLIRENKVHQIYSQMQLNQQ
NTGMVTQTQAMVKALRSNLITKENALRYSTNLQELIGIIGE

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=28801 CHAB381_RS07670 WP_012109472.1 1565458..1566543(+) (pilT) [Campylobacter hominis ATCC BAA-381]
ATGGTAGAAGAAAATCGCAAACATTTAGCGGATATTGAAACTCTTTTAAAAACAGTCGTTTTTAATAAAGCATCTGACTT
GCACCTTGTTTCGCGCTCGGCACCGCAAGTAAGAATAGATGGCAAGCTACGCCCATTAGCTATGGATGTTTTAACCGGTA
GCGATATAGAATATATTTGCTACGCTTTAATTACTGATGCGCAAAAAAGTGAATTGGAAGAAAATAAAGAGCTTGACTTT
GCGATAGAGCTTCCAAATATAGGTCGTTTTCGTGGAAATTACTATTATACGATGAACGGCGATTTAGCAGCTGCATTTCG
TATAATTCCAATAGACATTCCAAGCCTTGACGAATTAAAAGCGCCATCCATTTTTAAAGAAATCGTAAAACACGAAAAAG
GTATGATATTAGTTACAGGTCCTACAGGTAGCGGTAAATCAACAACTTTGGCAGCAATGCTAAATGAGATAAATGAAACT
GAGCGAAAGCACATTATCACGGTTGAAGATCCGGTTGAGTTCGTGCATACAAATAAACGTTCGCTTTTTTCGCATAGAAA
TATAGGTACAGATACACAATCTTACGCAAGAGCTTTAAAATTTTCACTACGTGAAGACCCGGATATAATTCTAGTAGGCG
AGCTTCGTGACCGCGAAACAATTTCTATTGCGATAACTGCAGCTGAAACAGGCCACTTGGTTTTTGGAACACTTCACACA
AACTCGGCAATTCAATCTCTAAACAGAATTATTGATAGTTTTGATGGCGGTGAACAACTTCAAGTAAGAAATATGTTATC
AGTTTCATTGACTTCCATTATTTCTCAATCTTTGCTTCCAAGAATTGGAAAAGGTCGTGTTGCAATACATGAAATTTTAA
TCAATAATAATGCGATTGCAAACCTTATACGTGAAAATAAAGTGCATCAAATCTATTCTCAAATGCAGTTAAATCAACAA
AATACAGGTATGGTAACACAAACTCAGGCAATGGTAAAAGCGCTTCGTTCGAATTTAATCACAAAAGAAAATGCGCTTCG
TTATTCTACAAATTTGCAAGAACTTATCGGAATTATAGGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7I3S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

51.884

95.568

0.496
  pilT Neisseria meningitidis 8013

51.594

95.568

0.493
  pilT Vibrio cholerae strain A1552

55.172

88.366

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.172

88.366

0.488
  pilT Legionella pneumophila strain Lp02

50.147

94.46

0.474
  pilT Legionella pneumophila strain ERS1305867

50.147

94.46

0.474
  pilT Acinetobacter baumannii D1279779

49.851

92.798

0.463
  pilT Acinetobacter nosocomialis M2

49.851

92.798

0.463
  pilT Acinetobacter baumannii strain A118

49.851

92.798

0.463
  pilT Acinetobacter baylyi ADP1

50

91.413

0.457
  pilT Pseudomonas aeruginosa PAK

47.761

92.798

0.443
  pilT Pseudomonas stutzeri DSM 10701

46.866

92.798

0.435
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.775

95.845

0.41
  pilU Pseudomonas stutzeri DSM 10701

38.617

96.122

0.371
  pilU Vibrio cholerae strain A1552

38.439

95.845

0.368

Multiple sequence alignment