Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GURA_RS21730 Genome accession   NC_009483
Coordinates   4935923..4936993 (+) Length   356 a.a.
NCBI ID   WP_011941047.1    Uniprot ID   A5G9E9
Organism   Geotalea uraniireducens Rf4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4930923..4941993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GURA_RS21715 (Gura_4271) - 4931503..4933080 (+) 1578 WP_011941044.1 PAS domain-containing sensor histidine kinase -
  GURA_RS21720 (Gura_4272) - 4933070..4934074 (-) 1005 WP_011941045.1 hypothetical protein -
  GURA_RS21725 (Gura_4273) - 4934250..4935908 (+) 1659 WP_011941046.1 GspE/PulE family protein -
  GURA_RS21730 (Gura_4274) pilT 4935923..4936993 (+) 1071 WP_011941047.1 type IV pilus twitching motility protein PilT Machinery gene
  GURA_RS21735 (Gura_4275) - 4937015..4937770 (-) 756 WP_011941048.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  GURA_RS21740 (Gura_4276) - 4937767..4938942 (-) 1176 WP_011941049.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  GURA_RS21745 (Gura_4277) - 4938923..4939900 (-) 978 WP_011941050.1 Gfo/Idh/MocA family protein -
  GURA_RS21750 (Gura_4278) - 4939996..4941447 (-) 1452 WP_232278955.1 NDP-hexose 2,3-dehydratase family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39667.92 Da        Isoelectric Point: 7.0824

>NTDB_id=28136 GURA_RS21730 WP_011941047.1 4935923..4936993(+) (pilT) [Geotalea uraniireducens Rf4]
MARIDALFKMMHEQGASDLHLSTGAPPIFRLHGEMERLNFKALAHEDLKSILYEILSEKQIADFEARKDLDFAYSVPGLA
RFRGNILMQHRGIAAVFRIIPSKILTADELNLPEGVRKLTMLRKGLVLVTGPTGSGKSTTLAAMIDLINSTRREHILTLE
DPLEFIHENKMSLFNQRQIGEHSESFAAALRAALREDPDVILVGEMRDLETISLAMSAAETGHLVFGTLHTSSAAKTVDR
IIDVFPKDAQEQVRAMLSESLKGVISQQLLKNADGKGRTAALEIMMGTPAIGNLIREGKTFQIPSIMQTARKDGMQLMDQ
HLLDLLKTKKVAPEEAYRCSADKKQFEQYLPDRPQQ

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=28136 GURA_RS21730 WP_011941047.1 4935923..4936993(+) (pilT) [Geotalea uraniireducens Rf4]
ATGGCACGCATTGACGCACTATTTAAAATGATGCACGAACAGGGAGCCTCGGACCTGCACCTCTCCACCGGTGCCCCCCC
CATCTTCCGCCTGCATGGAGAAATGGAGCGCCTGAACTTCAAGGCGCTGGCCCACGAGGACCTGAAGTCCATCCTTTACG
AGATCCTCAGCGAGAAACAGATAGCCGATTTCGAAGCGCGAAAGGACCTGGATTTCGCCTATTCCGTGCCGGGCCTCGCC
CGCTTCCGCGGCAACATCCTCATGCAGCATCGCGGGATTGCGGCAGTTTTCCGCATCATCCCCAGTAAAATCCTCACGGC
CGACGAGCTGAATCTCCCGGAAGGGGTGCGCAAGCTGACCATGCTGAGGAAGGGGCTGGTCCTCGTCACCGGCCCGACCG
GTTCCGGTAAATCCACCACCCTGGCGGCAATGATCGACCTGATAAACTCCACCCGCCGCGAACATATCCTCACCCTGGAA
GACCCGCTTGAATTCATCCACGAAAACAAGATGTCCCTCTTCAACCAGCGCCAGATTGGCGAGCATTCAGAGAGTTTCGC
CGCTGCGCTCCGCGCCGCCCTGCGTGAGGACCCGGACGTGATCCTCGTCGGCGAGATGCGCGACCTGGAGACCATCAGCC
TGGCCATGAGCGCCGCAGAGACCGGCCACCTCGTCTTCGGCACCCTCCACACCAGTTCCGCGGCCAAGACCGTGGACAGG
ATCATCGATGTTTTTCCCAAGGATGCCCAGGAGCAGGTGCGCGCCATGCTCTCCGAATCACTCAAGGGGGTCATCAGCCA
GCAGTTGCTGAAAAACGCAGACGGCAAGGGAAGGACGGCTGCCCTGGAGATCATGATGGGCACCCCGGCCATCGGCAACC
TGATCCGCGAGGGGAAAACGTTCCAGATCCCCTCCATCATGCAGACCGCCCGGAAAGACGGCATGCAGCTCATGGACCAG
CACCTGCTCGACCTGCTGAAGACGAAGAAAGTAGCCCCGGAAGAGGCATACCGCTGCTCCGCCGACAAGAAGCAGTTCGA
GCAGTACCTCCCTGACCGGCCGCAGCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A5G9E9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

55.62

97.472

0.542
  pilT Acinetobacter baumannii D1279779

55.62

97.472

0.542
  pilT Acinetobacter baumannii strain A118

55.62

97.472

0.542
  pilT Pseudomonas stutzeri DSM 10701

55.685

96.348

0.537
  pilT Pseudomonas aeruginosa PAK

56.156

93.539

0.525
  pilT Acinetobacter baylyi ADP1

59.807

87.36

0.522
  pilT Legionella pneumophila strain ERS1305867

55.287

92.978

0.514
  pilT Legionella pneumophila strain Lp02

55.287

92.978

0.514
  pilT Neisseria meningitidis 8013

56.347

90.73

0.511
  pilT Neisseria gonorrhoeae MS11

56.037

90.73

0.508
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.217

93.258

0.506
  pilT Vibrio cholerae strain A1552

54.217

93.258

0.506
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.997

98.034

0.48
  pilU Vibrio cholerae strain A1552

42.202

91.854

0.388
  pilU Pseudomonas stutzeri DSM 10701

39.039

93.539

0.365

Multiple sequence alignment