Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AM379_RS03965 Genome accession   NZ_CP027618
Coordinates   830193..831218 (-) Length   341 a.a.
NCBI ID   WP_044865461.1    Uniprot ID   -
Organism   Enterobacter cloacae complex sp. FDA-CDC-AR_0132     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825193..836218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM379_RS03935 (AM379_03930) - 826094..826591 (+) 498 WP_032670169.1 SprT family zinc-dependent metalloprotease -
  AM379_RS03940 (AM379_03935) endA 826686..827393 (+) 708 WP_106503478.1 deoxyribonuclease I -
  AM379_RS03945 (AM379_03940) rsmE 827446..828177 (+) 732 WP_020883697.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AM379_RS03950 (AM379_03945) gshB 828217..829164 (+) 948 WP_020883696.1 glutathione synthase -
  AM379_RS03955 (AM379_03950) - 829251..829811 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  AM379_RS03960 (AM379_03955) ruvX 829811..830227 (+) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  AM379_RS03965 (AM379_03960) pilT 830193..831218 (-) 1026 WP_044865461.1 type IV pilus twitching motility protein PilT Machinery gene
  AM379_RS03970 (AM379_03965) - 831236..831937 (+) 702 WP_014171569.1 YggS family pyridoxal phosphate-dependent enzyme -
  AM379_RS03975 (AM379_03970) - 831959..832525 (+) 567 WP_014171570.1 YggT family protein -
  AM379_RS03980 (AM379_03975) yggU 832522..832818 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  AM379_RS03985 (AM379_03980) - 832822..833415 (+) 594 WP_014171572.1 XTP/dITP diphosphatase -
  AM379_RS03990 (AM379_03985) hemW 833408..834550 (+) 1143 WP_106503479.1 radical SAM family heme chaperone HemW -
  AM379_RS03995 (AM379_03990) - 834614..834976 (+) 363 WP_106503480.1 endonuclease domain-containing protein -
  AM379_RS04000 (AM379_03995) - 835038..835754 (-) 717 WP_020883689.1 DUF2884 domain-containing protein -
  AM379_RS04005 (AM379_04000) - 835811..836137 (-) 327 WP_020883688.1 YggL family protein -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37147.20 Da        Isoelectric Point: 6.0946

>NTDB_id=278517 AM379_RS03965 WP_044865461.1 830193..831218(-) (pilT) [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLSVLGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=278517 AM379_RS03965 WP_044865461.1 830193..831218(-) (pilT) [Enterobacter cloacae complex sp. FDA-CDC-AR_0132]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCGCCCGAAGTCGATACGCTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTTACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCTCCGTGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGCTAATTCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTGGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTGCTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

99.12

0.487
  pilT Vibrio cholerae strain A1552

49.112

99.12

0.487
  pilT Neisseria meningitidis 8013

48.348

97.654

0.472
  pilT Neisseria gonorrhoeae MS11

46.784

100

0.469
  pilT Legionella pneumophila strain ERS1305867

49.068

94.428

0.463
  pilT Legionella pneumophila strain Lp02

49.068

94.428

0.463
  pilT Acinetobacter baylyi ADP1

46.988

97.361

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.706

95.894

0.457
  pilT Acinetobacter baumannii strain A118

47.401

95.894

0.455
  pilT Acinetobacter baumannii D1279779

47.401

95.894

0.455
  pilT Pseudomonas aeruginosa PAK

47.401

95.894

0.455
  pilT Acinetobacter nosocomialis M2

47.812

93.842

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.72

94.428

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.303

96.774

0.39
  pilU Vibrio cholerae strain A1552

39.441

94.428

0.372

Multiple sequence alignment