Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GTNG_RS13180 Genome accession   NC_009328
Coordinates   2658307..2659362 (-) Length   351 a.a.
NCBI ID   WP_011887915.1    Uniprot ID   -
Organism   Geobacillus thermodenitrificans NG80-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2653307..2664362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTNG_RS13150 (GTNG_2552) - 2653600..2654268 (-) 669 WP_011887912.1 pilus assembly protein PilO -
  GTNG_RS13155 (GTNG_2553) - 2654265..2654840 (-) 576 WP_008881082.1 PilN domain-containing protein -
  GTNG_RS13160 (GTNG_2554) pilM 2654830..2655762 (-) 933 WP_008881083.1 type IV pilus biogenesis protein PilM -
  GTNG_RS13165 (GTNG_2555) - 2655788..2656543 (-) 756 WP_008881084.1 prepilin peptidase -
  GTNG_RS13170 (GTNG_2556) - 2656543..2657022 (-) 480 WP_174375698.1 type II secretion system protein -
  GTNG_RS13175 (GTNG_2557) - 2657109..2658320 (-) 1212 WP_011887914.1 type II secretion system F family protein -
  GTNG_RS13180 (GTNG_2558) pilT 2658307..2659362 (-) 1056 WP_011887915.1 type IV pilus twitching motility protein PilT Machinery gene
  GTNG_RS13185 (GTNG_2559) - 2659375..2661039 (-) 1665 WP_008881088.1 GspE/PulE family protein -
  GTNG_RS13190 (GTNG_2560) - 2661036..2662403 (-) 1368 WP_008881089.1 VanW family protein -
  GTNG_RS13195 (GTNG_2561) - 2662773..2664119 (-) 1347 WP_011887916.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38721.78 Da        Isoelectric Point: 7.0292

>NTDB_id=27814 GTNG_RS13180 WP_011887915.1 2658307..2659362(-) (pilT) [Geobacillus thermodenitrificans NG80-2]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPPLWQQFQETGELDFSYGITGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRIAERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSVVANLIRNEKEHQIVNVMQTSKNVGMQTME
MSIKELVADGRIDPRAAEAYLVGERGEHGTI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=27814 GTNG_RS13180 WP_011887915.1 2658307..2659362(-) (pilT) [Geobacillus thermodenitrificans NG80-2]
ATGAAAGAAAAACTTGAGGCGTTATTACGTGCCGCCTTTGAATGCCGTGCGTCCGATGTGCATTTAACTGTCGGCGCACC
GCCGATTTTTCGTGTAGACGGGGAACTGAAAGCATACGGCCATGAGCGGCTTCGTCCGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCACCGCCGCTTTGGCAGCAGTTTCAAGAAACAGGGGAGCTCGACTTTTCGTACGGCATAACCGGAGTT
TCCCGCTTCCGTTTAAATGTGTTTAAACAGCGGTCGTGTGTTTCTTTAGCTGTGCGCCTCATTCCGATGCGCATCCCGAC
ACTTGAGGAGCTTGGCATGCCGGCCGTCTTAAAACGCATCGCCGAAAGGCCGCATGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGTGGTAAATCGACGACATTGGCAGCCATGATCGACTATATGAACAAAACGATGTCCAAACATATCATTACGCTC
GAAGATCCGATCGAATATTTACATAAACATGGAAAATCGATCATCGACCAGCGTGAAATCGGGTTTGACACCGGCAGCTT
TGCCGCTGGTTTGCGCGCTGCACTTCGCCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGATCACCGCCGCGGAAACGGGGCATTTCGTATTAGGCACGCTGCATACGGCAAGCGCGCCGGCGGCGATCGAG
CGGATGATTGACGTTTTTCCGTCGGCCCAGCAGACGCAAATTCGCTTGCAGCTCGCTTCCGTTCTTGTCGCAGTCATCGC
CCAACGTCTGTTTCCGAATAGCCGGGGGAACGGACGAGTGGCAGCGTTCGAAATTTTAATCAATAATTCCGTGGTCGCCA
ACTTAATTCGTAATGAAAAAGAACATCAAATCGTTAACGTGATGCAAACAAGCAAAAACGTTGGCATGCAGACGATGGAA
ATGAGCATCAAAGAGCTTGTGGCCGACGGGCGCATCGATCCACGTGCAGCCGAGGCGTATTTGGTAGGGGAGCGTGGCGA
ACATGGCACAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.292

90.883

0.484
  pilT Neisseria gonorrhoeae MS11

49.091

94.017

0.462
  pilT Neisseria meningitidis 8013

49.091

94.017

0.462
  pilT Pseudomonas aeruginosa PAK

49.536

92.023

0.456
  pilT Acinetobacter baylyi ADP1

48.193

94.587

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.605

95.157

0.453
  pilT Vibrio cholerae strain A1552

47.605

95.157

0.453
  pilT Acinetobacter baumannii strain A118

48.182

94.017

0.453
  pilT Acinetobacter baumannii D1279779

48.182

94.017

0.453
  pilT Acinetobacter nosocomialis M2

47.879

94.017

0.45
  pilT Pseudomonas stutzeri DSM 10701

47.72

93.732

0.447
  pilT Legionella pneumophila strain Lp02

46.364

94.017

0.436
  pilT Legionella pneumophila strain ERS1305867

46.364

94.017

0.436
  pilU Vibrio cholerae strain A1552

42.814

95.157

0.407
  pilU Pseudomonas stutzeri DSM 10701

42.249

93.732

0.396

Multiple sequence alignment