Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C4Q27_RS13395 Genome accession   NZ_CP026675
Coordinates   2777795..2778793 (+) Length   332 a.a.
NCBI ID   WP_104886948.1    Uniprot ID   A0AAU8YHX3
Organism   Pseudomonas sp. SWI36     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2772795..2783793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4Q27_RS13365 (C4Q27_13365) - 2773261..2773695 (-) 435 WP_020192921.1 DUF4426 domain-containing protein -
  C4Q27_RS13370 (C4Q27_13370) metW 2773718..2774338 (-) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  C4Q27_RS13375 (C4Q27_13375) - 2774346..2775485 (-) 1140 WP_010955639.1 homoserine O-acetyltransferase -
  C4Q27_RS13380 (C4Q27_13380) - 2775585..2776175 (-) 591 WP_003249110.1 YggT family protein -
  C4Q27_RS13385 (C4Q27_13385) proC 2776186..2777004 (-) 819 WP_004575483.1 pyrroline-5-carboxylate reductase -
  C4Q27_RS13390 (C4Q27_13390) - 2777043..2777729 (-) 687 WP_104886947.1 YggS family pyridoxal phosphate-dependent enzyme -
  C4Q27_RS13395 (C4Q27_13395) pilT 2777795..2778793 (+) 999 WP_104886948.1 type IV pilus twitching motility protein PilT Machinery gene
  C4Q27_RS13405 (C4Q27_13405) - 2778921..2779544 (-) 624 WP_020191289.1 C40 family peptidase -
  C4Q27_RS13410 (C4Q27_13410) - 2779709..2780128 (+) 420 WP_010955634.1 TM2 domain-containing protein -
  C4Q27_RS13415 (C4Q27_13415) - 2780219..2780917 (-) 699 WP_014859104.1 SPOR domain-containing protein -
  C4Q27_RS13420 (C4Q27_13420) argS 2780919..2782655 (-) 1737 WP_010955633.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36008.71 Da        Isoelectric Point: 7.5270

>NTDB_id=271600 C4Q27_RS13395 WP_104886948.1 2777795..2778793(+) (pilT) [Pseudomonas sp. SWI36]
MDVTDLLARAVDAGASDLHLATGQIPMLRLDGELQRMGLPPLNQGALIEGMASVLDDDQRRQWAQGDEMDLALELPALGR
FRLNLFRQLNGPAITFRLIPGRIATVSELDLADVFQAVAHCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEVIHASQRSLVNQREMGRHSRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSATSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLVREGRLAQLSSVMQGGAAEGMLTMEGAL
RRLRERGLIKGL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=271600 C4Q27_RS13395 WP_104886948.1 2777795..2778793(+) (pilT) [Pseudomonas sp. SWI36]
ATGGATGTGACCGACCTGTTGGCCCGAGCTGTGGATGCGGGGGCTTCAGACCTGCACCTGGCGACGGGCCAGATTCCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGTATGGGCTTGCCGCCGTTGAATCAGGGTGCCTTGATCGAGGGCATGGCCAGCG
TGCTGGATGACGATCAGCGCCGGCAGTGGGCCCAAGGCGATGAAATGGACCTTGCGCTGGAGTTGCCGGCGCTTGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGAATGGCCCGGCGATTACCTTTCGGCTGATCCCGGGGCGCATTGCGACGGTGAG
TGAGCTGGACCTTGCGGATGTGTTTCAAGCTGTTGCGCATTGCCGGGATGGCTTGATCCTCGTGGGCGGGCCGACCGGTA
GTGGCAAGTCCAGCACCCTGGCAACGCTGCTCGACCAGCTGAACCGCGAGCGGGCGCTGCACATCATTACCCTGGAAGAC
CCTGTCGAGGTCATCCATGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATGGGGCGCCATAGTCGCGGCTTTGCTCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CCCTGCGCGCGGCCGAAACCGGGCACCTGGTGCTGGCAACCGTGCATGCGCGTTCGGCGACCAGCAGCATCGACAGGTTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGCGTAGGGGGTGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGTAACCTGGTTC
GGGAGGGGCGGTTGGCGCAGTTGTCTTCAGTGATGCAGGGCGGGGCGGCGGAGGGGATGCTGACGATGGAGGGGGCGTTG
CGGAGGCTGAGGGAGCGAGGGTTGATCAAAGGCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Acinetobacter baumannii D1279779

50.456

99.096

0.5
  pilT Acinetobacter baumannii strain A118

50.456

99.096

0.5
  pilT Acinetobacter nosocomialis M2

50.456

99.096

0.5
  pilT Pseudomonas stutzeri DSM 10701

50.152

99.096

0.497
  pilT Acinetobacter baylyi ADP1

50

99.398

0.497
  pilT Neisseria meningitidis 8013

49.091

99.398

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria gonorrhoeae MS11

48.788

99.398

0.485
  pilT Legionella pneumophila strain ERS1305867

48.936

99.096

0.485
  pilT Legionella pneumophila strain Lp02

48.936

99.096

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.131

100

0.467
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373
  pilU Vibrio cholerae strain A1552

37.423

98.193

0.367

Multiple sequence alignment