Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CFF8240_RS05620 Genome accession   NC_008599
Coordinates   1135572..1136633 (-) Length   353 a.a.
NCBI ID   WP_002849776.1    Uniprot ID   A0A5L8UCG3
Organism   Campylobacter fetus subsp. fetus 82-40     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1130572..1141633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFF8240_RS05595 (CFF8240_1140) - 1131221..1131763 (-) 543 WP_002849765.1 DUF1882 domain-containing protein -
  CFF8240_RS05600 (CFF8240_1141) - 1131763..1133007 (-) 1245 WP_002849766.1 serine hydroxymethyltransferase -
  CFF8240_RS05605 (CFF8240_1142) lysS 1133004..1134515 (-) 1512 WP_024305203.1 lysine--tRNA ligase -
  CFF8240_RS05610 (CFF8240_1143) - 1134529..1134993 (-) 465 WP_011732095.1 Fur family transcriptional regulator -
  CFF8240_RS05615 (CFF8240_1144) - 1134997..1135572 (-) 576 WP_002849774.1 CvpA family protein -
  CFF8240_RS05620 (CFF8240_1145) pilT 1135572..1136633 (-) 1062 WP_002849776.1 type IV pilus twitching motility protein PilT Machinery gene
  CFF8240_RS09145 (CFF8240_1146) gatC 1136637..1136924 (-) 288 WP_002849777.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CFF8240_RS05630 (CFF8240_1147) - 1137107..1137706 (+) 600 WP_230454695.1 hypothetical protein -
  CFF8240_RS05635 (CFF8240_1148) - 1137709..1138716 (+) 1008 WP_002849780.1 hypothetical protein -
  CFF8240_RS05640 (CFF8240_1149) - 1138723..1139049 (+) 327 WP_002849781.1 hypothetical protein -
  CFF8240_RS05645 (CFF8240_1150) - 1139036..1139668 (+) 633 WP_002849782.1 type III pantothenate kinase -
  CFF8240_RS05650 (CFF8240_1151) hisG 1139656..1140267 (+) 612 WP_010400265.1 ATP phosphoribosyltransferase -
  CFF8240_RS05655 (CFF8240_1152) brnQ 1140276..1141580 (+) 1305 WP_011732097.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38935.38 Da        Isoelectric Point: 6.3450

>NTDB_id=26933 CFF8240_RS05620 WP_002849776.1 1135572..1136633(-) (pilT) [Campylobacter fetus subsp. fetus 82-40]
MNIETLLKTVMHNKASDLHLVGRSEPQIRIDGSLRPLEGKVLDGSDIENLCYTILTDAQKSELEENKDLDFALELPGIGR
FRGNCYYTMNGSLAAAFRMIPLNIPSLDDLNAPAIFKELVKKEKGLVLVTGGTGAGKSTTLAALLNEINITERKHIITIE
DPIEFVHQNKNSLFSHRNVGTDTKSFARALKYALREDPDIILVGDLRDPETISIAIAAAETGHLVFGTLHTNSAVQTINR
IVDSFNETEQAQVRNMLSVSLNAIISQTLVPKADNGRFAVHEVLINNHAISNLIRENKSHQIYSQIQLNQQNTGMQTQTQ
AMEKAVQMGIITKENAIRYSTNQQELAGKLGAI

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=26933 CFF8240_RS05620 WP_002849776.1 1135572..1136633(-) (pilT) [Campylobacter fetus subsp. fetus 82-40]
ATGAATATTGAAACTCTTTTAAAAACTGTGATGCATAACAAGGCGAGCGATCTACATTTAGTCGGTAGAAGTGAGCCTCA
AATCAGAATTGATGGTAGCTTAAGACCGCTTGAAGGTAAAGTTCTAGATGGAAGCGATATAGAAAATTTATGCTACACTA
TTTTAACTGATGCGCAAAAAAGCGAATTGGAAGAGAACAAGGATTTAGATTTTGCTCTAGAATTACCAGGAATAGGGCGT
TTTAGAGGTAATTGCTACTATACTATGAACGGAAGCTTAGCGGCTGCTTTTAGAATGATACCTCTAAATATACCAAGTCT
TGATGATCTTAATGCTCCGGCTATTTTTAAAGAGTTGGTAAAAAAAGAAAAAGGTCTTGTTCTTGTTACTGGAGGAACGG
GAGCTGGAAAATCGACGACTTTAGCAGCTTTGCTAAATGAGATAAATATTACTGAGCGAAAACATATAATCACCATAGAA
GATCCTATAGAATTTGTTCATCAAAATAAGAATTCTCTATTTTCTCATAGAAACGTAGGCACCGATACTAAATCGTTTGC
TAGGGCTTTGAAATACGCTCTTAGAGAAGATCCGGATATAATATTAGTAGGTGATTTAAGAGATCCGGAGACCATATCTA
TAGCTATAGCGGCCGCAGAAACAGGACACTTGGTATTTGGTACTTTGCACACAAACTCAGCCGTACAAACAATAAATAGA
ATAGTCGATAGTTTTAATGAGACAGAGCAGGCTCAAGTAAGAAATATGCTTAGCGTATCTCTAAATGCTATTATATCTCA
AACTCTTGTGCCAAAAGCAGATAACGGCAGATTTGCCGTTCATGAAGTTTTGATAAATAATCATGCAATTTCAAATTTGA
TCAGAGAAAATAAATCTCATCAAATTTACTCTCAAATACAATTAAATCAACAAAATACAGGTATGCAAACACAAACTCAA
GCGATGGAAAAGGCGGTGCAAATGGGTATTATAACAAAAGAAAACGCTATAAGATATTCAACAAATCAGCAAGAATTAGC
TGGAAAGTTAGGTGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5L8UCG3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50.287

98.584

0.496
  pilT Legionella pneumophila strain Lp02

50.729

97.167

0.493
  pilT Legionella pneumophila strain ERS1305867

50.729

97.167

0.493
  pilT Neisseria meningitidis 8013

50

98.584

0.493
  pilT Vibrio cholerae strain A1552

51.964

93.768

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.964

93.768

0.487
  pilT Acinetobacter baumannii D1279779

49.565

97.734

0.484
  pilT Acinetobacter nosocomialis M2

49.565

97.734

0.484
  pilT Acinetobacter baumannii strain A118

49.565

97.734

0.484
  pilT Pseudomonas aeruginosa PAK

49.702

95.184

0.473
  pilT Acinetobacter baylyi ADP1

50.151

93.768

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.81

95.184

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.092

98.584

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.402
  pilU Acinetobacter baylyi ADP1

39.181

96.884

0.38

Multiple sequence alignment