Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   SFRI_RS21815 Genome accession   NC_008345
Coordinates   4503482..4503910 (+) Length   142 a.a.
NCBI ID   WP_049763611.1    Uniprot ID   -
Organism   Shewanella frigidimarina NCIMB 400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4498482..4508910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SFRI_RS19400 (Sfri_3778) pdhR 4498584..4499336 (-) 753 WP_011639192.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  SFRI_RS19405 (Sfri_3779) ampE 4499635..4500486 (-) 852 WP_011639193.1 beta-lactamase regulator AmpE -
  SFRI_RS19410 (Sfri_3780) ampD 4500612..4501166 (-) 555 WP_011639194.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SFRI_RS19415 (Sfri_3781) nadC 4501586..4502464 (+) 879 WP_011639195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SFRI_RS21815 (Sfri_3782) pilE 4503482..4503910 (+) 429 WP_049763611.1 pilin Machinery gene
  SFRI_RS19425 (Sfri_3783) pilB 4504003..4505709 (+) 1707 WP_011639197.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SFRI_RS19430 (Sfri_3784) pilC 4505817..4507076 (+) 1260 WP_011639198.1 type II secretion system F family protein Machinery gene
  SFRI_RS19435 (Sfri_3785) pilD 4507106..4508032 (+) 927 WP_011639199.1 A24 family peptidase Machinery gene
  SFRI_RS19440 (Sfri_3786) coaE 4508029..4508658 (+) 630 WP_011639200.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14449.68 Da        Isoelectric Point: 6.9438

>NTDB_id=26355 SFRI_RS21815 WP_049763611.1 4503482..4503910(+) (pilE) [Shewanella frigidimarina NCIMB 400]
MKNAKGFTLIELMIVVAIIGILAAIALPAYQDFTKRGHVSEGLSLAAGAKASVTEYYATQGAFPTTNALAGLAAATDIKG
NAVVSVTVGALGQITVLFNDKVLATSTLTLTPSLAANSTGSISWTCAAGTAMEDAWIPSNCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=26355 SFRI_RS21815 WP_049763611.1 4503482..4503910(+) (pilE) [Shewanella frigidimarina NCIMB 400]
ATCAAAAACGCTAAGGGTTTCACTCTTATCGAATTAATGATCGTAGTCGCGATCATCGGTATTTTAGCTGCAATCGCACT
GCCTGCTTATCAAGACTTCACTAAACGTGGTCATGTTTCTGAAGGTTTAAGTTTAGCTGCTGGAGCAAAAGCTTCAGTGA
CAGAATATTACGCGACTCAAGGTGCATTTCCTACAACCAACGCACTTGCAGGGCTTGCTGCTGCTACGGATATTAAAGGT
AATGCTGTAGTCAGTGTAACAGTTGGAGCTCTTGGACAAATCACAGTTTTGTTTAATGACAAAGTTTTAGCAACTTCTAC
ATTAACTTTAACTCCATCACTTGCAGCAAATTCGACAGGAAGTATATCTTGGACATGTGCTGCTGGTACAGCAATGGAAG
ATGCTTGGATTCCATCAAACTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

43.949

100

0.486
  pilA2 Legionella pneumophila str. Paris

48.551

97.183

0.472
  pilA2 Legionella pneumophila strain ERS1305867

48.551

97.183

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

40.606

100

0.472
  pilE Neisseria gonorrhoeae MS11

42.138

100

0.472
  pilA/pilA1 Eikenella corrodens VA1

41.83

100

0.451
  comP Acinetobacter baylyi ADP1

42.384

100

0.451
  pilA Vibrio cholerae C6706

39.865

100

0.415
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.865

100

0.415
  pilA Vibrio cholerae strain A1552

39.865

100

0.415
  pilA Acinetobacter baumannii strain A118

37.162

100

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.298

99.296

0.38
  pilA Vibrio parahaemolyticus RIMD 2210633

40.152

92.958

0.373
  pilA Haemophilus influenzae 86-028NP

43.443

85.915

0.373

Multiple sequence alignment