Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CU052_RS08330 Genome accession   NZ_CP025537
Coordinates   1726262..1727368 (+) Length   368 a.a.
NCBI ID   WP_101904468.1    Uniprot ID   -
Organism   Vibrio harveyi strain 345     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1721262..1732368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU052_RS08295 (CU052_08695) - 1721401..1722003 (-) 603 WP_017189094.1 XTP/dITP diphosphatase -
  CU052_RS08300 (CU052_08700) - 1722109..1722540 (-) 432 WP_101904465.1 DUF4426 domain-containing protein -
  CU052_RS08305 (CU052_08705) yggU 1722600..1722890 (-) 291 WP_101904466.1 DUF167 family protein YggU -
  CU052_RS08310 (CU052_08710) - 1722890..1723447 (-) 558 WP_005425806.1 YggT family protein -
  CU052_RS08315 (CU052_08715) proC 1723501..1724319 (-) 819 WP_050904301.1 pyrroline-5-carboxylate reductase -
  CU052_RS08320 (CU052_08720) - 1724451..1725161 (-) 711 WP_101904467.1 YggS family pyridoxal phosphate-dependent enzyme -
  CU052_RS08325 (CU052_08725) pilT 1725190..1726230 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  CU052_RS08330 (CU052_08730) pilU 1726262..1727368 (+) 1107 WP_101904468.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CU052_RS08335 (CU052_08735) ruvX 1727428..1727853 (-) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  CU052_RS08340 (CU052_08740) - 1727903..1728466 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  CU052_RS08345 (CU052_08745) gshB 1728596..1729546 (-) 951 WP_005449741.1 glutathione synthase -
  CU052_RS08350 (CU052_08750) rsmE 1729561..1730292 (-) 732 WP_101904469.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CU052_RS08355 (CU052_08755) endA 1730439..1731134 (-) 696 WP_005449743.1 deoxyribonuclease I -
  CU052_RS08365 (CU052_08765) - 1731286..1731783 (-) 498 WP_101904470.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41295.43 Da        Isoelectric Point: 6.7729

>NTDB_id=262401 CU052_RS08330 WP_101904468.1 1726262..1727368(+) (pilU) [Vibrio harveyi strain 345]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLMLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=262401 CU052_RS08330 WP_101904468.1 1726262..1727368(+) (pilU) [Vibrio harveyi strain 345]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCGA
TGGACCCGGATCGACGACAGGAGTTTCGCAAAAGCCGCGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTGATGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAATTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter baylyi ADP1

41.279

93.478

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.351

92.935

0.375
  pilT Vibrio cholerae strain A1552

40.419

90.761

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.761

0.367

Multiple sequence alignment