Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CWI88_RS03755 Genome accession   NZ_CP025225
Coordinates   777174..778154 (+) Length   326 a.a.
NCBI ID   WP_101737001.1    Uniprot ID   -
Organism   Enterobacter cancerogenus strain CR-Eb1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772174..783154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI88_RS03715 (CWI88_03715) - 772235..772561 (+) 327 WP_006178455.1 YggL family protein -
  CWI88_RS03720 (CWI88_03720) - 772619..773335 (+) 717 WP_058608626.1 DUF2884 domain-containing protein -
  CWI88_RS03725 (CWI88_03725) - 773440..773778 (-) 339 WP_101738557.1 endonuclease domain-containing protein -
  CWI88_RS03730 (CWI88_03730) hemW 773842..774984 (-) 1143 WP_058608625.1 radical SAM family heme chaperone HemW -
  CWI88_RS03735 (CWI88_03735) - 774977..775570 (-) 594 WP_101736999.1 XTP/dITP diphosphatase -
  CWI88_RS03740 (CWI88_03740) yggU 775574..775870 (-) 297 WP_006178450.1 DUF167 family protein YggU -
  CWI88_RS03745 (CWI88_03745) - 775867..776433 (-) 567 WP_058608623.1 YggT family protein -
  CWI88_RS03750 (CWI88_03750) - 776455..777156 (-) 702 WP_101737000.1 YggS family pyridoxal phosphate-dependent enzyme -
  CWI88_RS03755 (CWI88_03755) pilT 777174..778154 (+) 981 WP_101737001.1 type IV pilus twitching motility protein PilT Machinery gene
  CWI88_RS03760 (CWI88_03760) ruvX 778186..778602 (-) 417 WP_006178446.1 Holliday junction resolvase RuvX -
  CWI88_RS03765 (CWI88_03765) - 778602..779162 (-) 561 WP_006178445.1 YqgE/AlgH family protein -
  CWI88_RS03770 (CWI88_03770) gshB 779245..780192 (-) 948 WP_101738558.1 glutathione synthase -
  CWI88_RS03775 (CWI88_03775) rsmE 780214..780945 (-) 732 WP_042321930.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CWI88_RS03780 (CWI88_03780) endA 780998..781705 (-) 708 WP_006178442.1 deoxyribonuclease I -
  CWI88_RS03785 (CWI88_03785) - 781800..782297 (-) 498 WP_101737002.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35800.80 Da        Isoelectric Point: 6.0414

>NTDB_id=259136 CWI88_RS03755 WP_101737001.1 777174..778154(+) (pilT) [Enterobacter cancerogenus strain CR-Eb1]
MDVEEIVALSVKHNVSDLHLCSDAPPRWRRLGRLEPAPFPPPDVNVLLREWLNDEQQGTWWANGQVDFAVTTGENQRLRG
SAFMHMQGASVALRLLPRACPQLSTLGVPRAIPELLLNDSGLLLVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPTFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRTQLAGSLRAVLAQKLRPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIVQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=259136 CWI88_RS03755 WP_101737001.1 777174..778154(+) (pilT) [Enterobacter cancerogenus strain CR-Eb1]
ATGGATGTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATGCCCCCCCGCG
CTGGCGGCGATTAGGCCGCCTTGAGCCCGCCCCGTTTCCGCCTCCGGACGTGAACGTGCTGCTCAGGGAGTGGCTTAATG
ATGAACAGCAGGGAACGTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACCACCGGCGAAAACCAGCGCCTGCGCGGT
AGCGCGTTTATGCATATGCAGGGTGCCTCTGTCGCGCTGCGACTGCTGCCGCGTGCCTGCCCGCAACTTTCTACGCTGGG
CGTGCCGCGGGCGATCCCGGAGCTGTTACTGAATGACAGCGGGCTGCTGCTGGTGACCGGGGCAACCGGCAGCGGAAAAT
CCACTACTCTGGCGGCGATGGTCGATTTTCTCAACCACCACACCGACGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTATCTATCAGAGTGAACGCTGCCTGATCCAGCAGCGCGAGATAGGCCAGCACAGCCCGACGTTTGCCGAGGCGCTGCG
GGGCGCGTTGCGTGAAGATCCGGATGTGATTTTGTTGGGGGAATTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGGGCATCGCAGGCCATTGAACGGCTGGTGGATACC
TTTCCGGCGCAGGAGAAAGACCCGGTGCGCACCCAGCTGGCGGGCAGCCTGCGGGCGGTTCTGGCGCAAAAGCTGCGCCC
GGATTTGCAGGGCGGGCGCGTCGCGCTTTATGAGCTGCTGGTGAATACCCCGGCGGCGGCGAACCTGATCCGTGAAGGCA
AAACCTGGCAGCTTCCCGGGATCGTTCAAACGGGTCAGCAGGCGGGCATGCAGAACTTTGAACAAAGCCTTGCTGAGCGC
CGGGCGCAGGGCAGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Legionella pneumophila strain Lp02

47.516

98.773

0.469
  pilT Legionella pneumophila strain ERS1305867

47.516

98.773

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.236

98.773

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374

Multiple sequence alignment