Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSEEN_RS01455 Genome accession   NC_008027
Coordinates   329488..330480 (+) Length   330 a.a.
NCBI ID   WP_011531740.1    Uniprot ID   Q1IGC2
Organism   Pseudomonas entomophila L48     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324488..335480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEEN_RS01425 (PSEEN0313) - 324962..325396 (-) 435 WP_011531734.1 DUF4426 domain-containing protein -
  PSEEN_RS01430 (PSEEN0314) metW 325422..326042 (-) 621 WP_011531735.1 methionine biosynthesis protein MetW -
  PSEEN_RS01435 (PSEEN0315) metX 326050..327189 (-) 1140 WP_011531736.1 homoserine O-succinyltransferase MetX -
  PSEEN_RS01440 (PSEEN0316) - 327278..327868 (-) 591 WP_011531737.1 YggT family protein -
  PSEEN_RS01445 (PSEEN0317) proC 327879..328697 (-) 819 WP_011531738.1 pyrroline-5-carboxylate reductase -
  PSEEN_RS01450 (PSEEN0318) - 328736..329422 (-) 687 WP_011531739.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSEEN_RS01455 (PSEEN0319) pilT 329488..330480 (+) 993 WP_011531740.1 type IV pilus twitching motility protein PilT Machinery gene
  PSEEN_RS01460 (PSEEN0320) - 330607..331254 (-) 648 WP_011531741.1 C40 family peptidase -
  PSEEN_RS01465 (PSEEN0321) - 331413..331838 (+) 426 WP_011531742.1 NINE protein -
  PSEEN_RS01470 (PSEEN0322) - 331877..332581 (-) 705 WP_011531743.1 SPOR domain-containing protein -
  PSEEN_RS01475 (PSEEN0323) argS 332583..334319 (-) 1737 WP_011531744.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 35890.29 Da        Isoelectric Point: 5.9861

>NTDB_id=25898 PSEEN_RS01455 WP_011531740.1 329488..330480(+) (pilT) [Pseudomonas entomophila L48]
MDVTDLLAQAVAAGASDLHLAAGEVPLLRLDGELRRMDMPVLMPAALEQGVAGLLDDGQRRQWASGDELDLALAVPALGR
FRVNLFRQRNGPGASIRLIPARVPSLDELDLHDVFQTIASNRDGLVLIGGPTGSGKSSTLAALVDQLNREYALHIVTLED
PVEFIHQGQRSLVNQREIGRDSRDFSQGLRSALRQDPDVIMVGELRDLESIRLALQAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRTMLAESLRLVVAQRLVRQVGGGRVAAREVLVVTPAVRNLIREGRMAQICSLMQVGAAQGMVTMEEAT
RRLRQQGLID

Nucleotide


Download         Length: 993 bp        

>NTDB_id=25898 PSEEN_RS01455 WP_011531740.1 329488..330480(+) (pilT) [Pseudomonas entomophila L48]
ATGGATGTGACCGATCTGCTGGCCCAGGCTGTGGCTGCGGGCGCAAGCGACCTGCACCTGGCCGCGGGCGAGGTACCGTT
GCTGCGTTTGGACGGTGAGCTGCGGCGGATGGACATGCCGGTACTCATGCCTGCGGCGCTGGAGCAGGGCGTGGCTGGCT
TGCTCGATGATGGGCAGCGCCGACAGTGGGCGTCTGGTGACGAGCTGGATCTTGCCTTGGCGGTGCCCGCCTTGGGCCGC
TTCCGGGTGAACCTGTTTCGCCAACGTAATGGGCCGGGGGCGAGCATACGGCTGATTCCGGCGCGGGTGCCAAGCCTCGA
TGAGCTCGACCTGCATGATGTATTTCAAACTATTGCATCGAACCGGGATGGCCTGGTGCTGATTGGCGGGCCTACGGGGA
GCGGCAAGTCCAGCACCCTGGCTGCGCTGGTCGATCAGCTCAATCGGGAGTACGCGCTTCACATCGTCACCTTGGAGGAC
CCTGTCGAATTCATTCATCAAGGCCAGCGCAGTCTGGTCAACCAACGGGAAATTGGTCGCGACAGTCGCGATTTTTCCCA
GGGATTGCGCAGTGCGTTGCGCCAGGATCCCGACGTAATCATGGTCGGTGAGTTGCGTGACCTGGAGAGTATCCGTCTGG
CGCTGCAAGCTGCGGAAACCGGGCACTTGGTGTTGGCCACGGTGCATACACGCTCGGCGGTCAACAGTATCGACCGCCTG
GTGGAGGTGTTTGCCGCCGAGGAGAAGCCGCTGGTGCGCACGATGTTGGCGGAGTCGTTGCGGCTGGTGGTGGCGCAGAG
GCTGGTCAGGCAGGTCGGTGGCGGCAGGGTCGCCGCAAGGGAGGTGCTGGTGGTGACGCCGGCGGTGCGCAACCTGATCC
GCGAGGGGCGGATGGCGCAGATCTGTTCGTTGATGCAGGTCGGGGCGGCGCAGGGGATGGTGACGATGGAGGAGGCGACA
CGACGCCTGAGGCAGCAAGGCTTGATCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1IGC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.818

100

0.518
  pilT Pseudomonas aeruginosa PAK

51.672

99.697

0.515
  pilT Acinetobacter baumannii D1279779

51.368

99.697

0.512
  pilT Acinetobacter baumannii strain A118

51.368

99.697

0.512
  pilT Pseudomonas stutzeri DSM 10701

51.368

99.697

0.512
  pilT Acinetobacter nosocomialis M2

51.368

99.697

0.512
  pilT Vibrio cholerae strain A1552

50.303

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.303

100

0.503
  pilT Neisseria meningitidis 8013

49.091

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.788

100

0.488
  pilT Legionella pneumophila strain ERS1305867

48.936

99.697

0.488
  pilT Legionella pneumophila strain Lp02

48.936

99.697

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.484

96.364

0.458
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.391
  pilU Vibrio cholerae strain A1552

37.654

98.182

0.37

Multiple sequence alignment