Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CWI22_RS25145 Genome accession   NZ_CP025053
Coordinates   5228356..5228808 (-) Length   150 a.a.
NCBI ID   WP_023518088.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB354     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5223356..5233808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI22_RS25125 (CWI22_25125) ampD 5224051..5224617 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CWI22_RS25130 (CWI22_25130) - 5224768..5227023 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  CWI22_RS25135 (CWI22_25135) nadC 5227309..5228157 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI22_RS25145 (CWI22_25145) pilA 5228356..5228808 (-) 453 WP_023518088.1 pilin Machinery gene
  CWI22_RS25150 (CWI22_25150) pilB 5229040..5230740 (+) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI22_RS25155 (CWI22_25155) pilC 5230744..5231964 (+) 1221 WP_031634696.1 type 4a pilus biogenesis protein PilC Machinery gene
  CWI22_RS25160 (CWI22_25160) pilD 5231968..5232840 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI22_RS25165 (CWI22_25165) coaE 5232837..5233448 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  CWI22_RS25170 (CWI22_25170) yacG 5233445..5233645 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15686.02 Da        Isoelectric Point: 7.7814

>NTDB_id=257812 CWI22_RS25145 WP_023518088.1 5228356..5228808(-) (pilA) [Pseudomonas aeruginosa strain PB354]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGTEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=257812 CWI22_RS25145 WP_023518088.1 5228356..5228808(-) (pilA) [Pseudomonas aeruginosa strain PB354]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGCTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.651

99.333

0.473
  pilA Vibrio cholerae strain A1552

47.651

99.333

0.473
  pilA Vibrio cholerae C6706

47.651

99.333

0.473
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.333

100

0.453
  pilA Acinetobacter baumannii strain A118

44.595

98.667

0.44
  pilA Vibrio parahaemolyticus RIMD 2210633

43.243

98.667

0.427
  comP Acinetobacter baylyi ADP1

42

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

39.597

99.333

0.393
  pilA2 Legionella pneumophila str. Paris

38.926

99.333

0.387
  pilA/pilA1 Eikenella corrodens VA1

38.514

98.667

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

38.889

96

0.373
  pilA Acinetobacter nosocomialis M2

41.353

88.667

0.367

Multiple sequence alignment