Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CWI22_RS25155 Genome accession   NZ_CP025053
Coordinates   5230744..5231964 (+) Length   406 a.a.
NCBI ID   WP_031634696.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PB354     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5225744..5236964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI22_RS25135 (CWI22_25135) nadC 5227309..5228157 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI22_RS25145 (CWI22_25145) pilA 5228356..5228808 (-) 453 WP_023518088.1 pilin Machinery gene
  CWI22_RS25150 (CWI22_25150) pilB 5229040..5230740 (+) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI22_RS25155 (CWI22_25155) pilC 5230744..5231964 (+) 1221 WP_031634696.1 type 4a pilus biogenesis protein PilC Machinery gene
  CWI22_RS25160 (CWI22_25160) pilD 5231968..5232840 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI22_RS25165 (CWI22_25165) coaE 5232837..5233448 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  CWI22_RS25170 (CWI22_25170) yacG 5233445..5233645 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CWI22_RS25175 (CWI22_25175) - 5233682..5233891 (-) 210 WP_003094660.1 hypothetical protein -
  CWI22_RS25180 (CWI22_25180) - 5233997..5234686 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  CWI22_RS25185 (CWI22_25185) - 5234683..5235153 (-) 471 WP_003094664.1 hypothetical protein -
  CWI22_RS25190 (CWI22_25190) - 5235150..5235575 (-) 426 WP_003116252.1 GNAT family N-acetyltransferase -
  CWI22_RS25195 (CWI22_25195) - 5235708..5236337 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  CWI22_RS25200 (CWI22_25200) - 5236334..5236783 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44270.00 Da        Isoelectric Point: 9.8784

>NTDB_id=257814 CWI22_RS25155 WP_031634696.1 5230744..5231964(+) (pilC) [Pseudomonas aeruginosa strain PB354]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPIDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=257814 CWI22_RS25155 WP_031634696.1 5230744..5231964(+) (pilC) [Pseudomonas aeruginosa strain PB354]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATAGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAATTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.79

99.754

0.766
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367

Multiple sequence alignment