Detailed information NTG0024960

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: GMET_RS17045
• Genome accession: NC_007517
• Coordinates: 3814163..3815233 (-)
• Length: 356 a.a.
• NCBI ID: WP_004514207.1
• Uniprot ID: Q39Q62
• Organism: Geobacter metallireducens GS-15
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3809163..3820233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GMET_RS17020 (Gmet_3396)	-	3810050..3811273 (+)	1224	WP_011365668.1	ISL3-like element ISGme5 family transposase	-
GMET_RS17030 (Gmet_3397)	-	3811547..3811837 (+)	291	WP_004514204.1	hypothetical protein	-
GMET_RS17035 (Gmet_3398)	-	3811888..3812655 (+)	768	WP_004514205.1	alpha/beta fold hydrolase	-
GMET_RS17040 (Gmet_3399)	-	3812707..3814023 (-)	1317	WP_004514206.1	hypothetical protein	-
GMET_RS17045 (Gmet_3400)	pilT	3814163..3815233 (-)	1071	WP_004514207.1	type IV pilus twitching motility protein PilT	Machinery gene
GMET_RS17050 (Gmet_3401)	-	3815246..3816868 (-)	1623	WP_004514208.1	GspE/PulE family protein	-
GMET_RS17055 (Gmet_3402)	-	3817052..3818056 (+)	1005	WP_004514209.1	hypothetical protein	-
GMET_RS17060 (Gmet_3403)	-	3818269..3819231 (-)	963	WP_011366187.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39707.96 Da
• Isoelectric Point: 7.3019

>NTDB_id=24960 GMET_RS17045 WP_004514207.1 3814163..3815233(-) (pilT) [Geobacter metallireducens GS-15]
MARIDTLFKMLKDQGASDLHLSSGAPPIFRLHGEMARQNFKPLTHDELKAILYEILTEKQKADFEERHDLDFAYAIPGLA
RFRGNYMLTHRGIAAVFRIIPSKILSADDLSLPDGVRRMTLFKKGLVLVTGPTGSGKSTTLAAMIDLINATRKEHILTLE
DPLEFIHENKMSLLNQRQIGEHSMTFTAALRAALREDPDVILVGEMRDLETIGLAMSAAETGHLVFGTLHTNSAAKTIDR
IIDVFPTDQQEQTRAMLSESLKGVVCQQLLKTADGKGRVAALEIMLGTPAIGNLIREGKTFQIPSIIQTAKRDGMQLMDQ
HLLDLFKTKRITAEEAYRCAQDKKQFEQYLAEKPAQ

Nucleotide

• Length: 1071 bp

>NTDB_id=24960 GMET_RS17045 WP_004514207.1 3814163..3815233(-) (pilT) [Geobacter metallireducens GS-15]
ATGGCACGCATCGACACACTCTTTAAAATGCTCAAGGATCAGGGGGCTTCCGACCTCCACCTCTCCTCCGGGGCGCCCCC
CATCTTCCGGCTCCACGGCGAGATGGCCCGCCAGAACTTCAAGCCTCTCACCCATGACGAGCTGAAGGCCATCCTCTACG
AGATCCTCACCGAGAAGCAGAAGGCCGACTTCGAGGAGCGCCACGACCTCGACTTCGCCTACGCCATCCCGGGGCTCGCC
CGGTTCCGGGGGAACTACATGCTGACCCACCGGGGGATCGCCGCGGTCTTCCGGATCATCCCCAGCAAGATCCTCTCGGC
CGACGATCTCAGCCTCCCCGACGGGGTCCGGCGCATGACCCTGTTCAAGAAGGGGCTCGTGCTCGTCACCGGCCCCACCG
GCTCGGGGAAATCCACCACCCTGGCCGCCATGATCGACCTCATCAACGCAACCCGCAAGGAGCACATCCTCACCCTGGAG
GACCCCCTCGAATTCATCCACGAGAACAAGATGTCGCTCCTGAACCAGCGGCAGATCGGCGAACACTCCATGACCTTCAC
CGCTGCCCTGCGGGCAGCCCTGCGGGAAGACCCCGACGTCATCCTCGTGGGGGAGATGCGGGACCTGGAGACCATCGGCC
TCGCCATGAGTGCCGCCGAGACCGGCCACCTGGTCTTCGGGACGCTGCACACCAACTCGGCGGCCAAGACCATCGACCGG
ATCATCGACGTCTTCCCCACCGACCAGCAGGAGCAGACCCGGGCCATGCTCTCCGAATCCCTCAAGGGGGTCGTCTGCCA
GCAGCTCCTGAAGACCGCCGACGGCAAGGGGCGGGTGGCGGCCCTGGAGATCATGCTCGGCACCCCGGCCATCGGCAACC
TGATCCGCGAGGGGAAGACCTTCCAGATCCCCTCCATCATCCAGACCGCCAAGCGGGACGGGATGCAGCTCATGGACCAG
CACCTCCTGGACCTCTTCAAGACCAAGCGGATCACCGCCGAAGAGGCCTACCGCTGCGCTCAGGACAAGAAACAGTTCGA
GCAGTATTTGGCCGAGAAGCCGGCCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (8-271)

3D structure

• AlphaFold DB: Q39Q62

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Legionella pneumophila strain Lp02	57.704	92.978	0.537
pilT	Legionella pneumophila strain ERS1305867	57.704	92.978	0.537
pilT	Pseudomonas stutzeri DSM 10701	55.394	96.348	0.534
pilT	Pseudomonas aeruginosa PAK	55.102	96.348	0.531
pilT	Acinetobacter baylyi ADP1	56.193	92.978	0.522
pilT	Acinetobacter baumannii D1279779	55.255	93.539	0.517
pilT	Acinetobacter nosocomialis M2	55.255	93.539	0.517
pilT	Acinetobacter baumannii strain A118	55.255	93.539	0.517
pilT	Neisseria meningitidis 8013	54.711	92.416	0.506
pilT	Neisseria gonorrhoeae MS11	54.711	92.416	0.506
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	52.187	96.348	0.503
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	52.711	93.258	0.492
pilT	Vibrio cholerae strain A1552	52.711	93.258	0.492
pilU	Pseudomonas stutzeri DSM 10701	39.458	93.258	0.368
pilU	Acinetobacter baylyi ADP1	38.576	94.663	0.365