Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CO697_RS12660 Genome accession   NZ_CP023529
Coordinates   2594646..2595626 (-) Length   326 a.a.
NCBI ID   WP_059177572.1    Uniprot ID   -
Organism   Lelliottia amnigena strain FDAARGOS_395     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2589646..2600626
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CO697_RS12640 (CO697_12640) - 2590536..2591096 (+) 561 WP_059177576.1 YqgE/AlgH family protein -
  CO697_RS12645 (CO697_12645) ruvX 2591096..2591512 (+) 417 WP_059177575.1 Holliday junction resolvase RuvX -
  CO697_RS12650 (CO697_12650) - 2591631..2592323 (-) 693 WP_059177574.1 IclR family transcriptional regulator -
  CO697_RS12655 (CO697_12655) - 2592391..2594649 (+) 2259 WP_059177573.1 FAD-dependent oxidoreductase -
  CO697_RS12660 (CO697_12660) pilT 2594646..2595626 (-) 981 WP_059177572.1 type IV pilus twitching motility protein PilT Machinery gene
  CO697_RS12665 (CO697_12665) - 2595644..2596345 (+) 702 WP_059177571.1 YggS family pyridoxal phosphate-dependent enzyme -
  CO697_RS12670 (CO697_12670) - 2596367..2596933 (+) 567 WP_059177570.1 YggT family protein -
  CO697_RS12675 (CO697_12675) yggU 2596930..2597220 (+) 291 WP_059177569.1 DUF167 family protein YggU -
  CO697_RS12680 (CO697_12680) - 2597229..2597822 (+) 594 WP_059177568.1 XTP/dITP diphosphatase -
  CO697_RS12685 (CO697_12685) hemW 2597815..2598957 (+) 1143 WP_059177567.1 radical SAM family heme chaperone HemW -
  CO697_RS12690 (CO697_12690) - 2599021..2599359 (+) 339 WP_059177566.1 endonuclease domain-containing protein -
  CO697_RS12695 (CO697_12695) - 2599543..2600262 (-) 720 WP_059177565.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35642.59 Da        Isoelectric Point: 6.1200

>NTDB_id=248161 CO697_RS12660 WP_059177572.1 2594646..2595626(-) (pilT) [Lelliottia amnigena strain FDAARGOS_395]
MDMEEIVALSVKHNVSDLHLCSNSPPRWRRLGHLESAPFPAPDVQLLLKAWLNDEQEGAWWANGQVDFAVSMNSGQRLRA
SAFNHIHGTSITLRLLPVTCPTLSELTAPKVIPDLLSSDNGLILVTGATGSGKSTTLAAMVDHLNSHCEGHILTLEDPVE
FIYQSNRCLIQQREIGLHCPSFSQALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAEEKDPVRNQLAGSLRAVLAQKLERDAQGGRVALFEMLVNTPASANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
KAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=248161 CO697_RS12660 WP_059177572.1 2594646..2595626(-) (pilT) [Lelliottia amnigena strain FDAARGOS_395]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGCAATTCACCACCGCG
CTGGCGTCGTTTGGGTCATCTTGAATCTGCACCTTTTCCCGCCCCGGATGTTCAGCTGCTCCTTAAAGCCTGGCTCAACG
ACGAGCAGGAGGGGGCATGGTGGGCGAATGGGCAGGTGGATTTCGCCGTATCGATGAACAGCGGGCAACGCTTGCGCGCC
AGCGCGTTTAACCATATTCATGGCACCTCGATAACGCTCAGATTGTTGCCAGTGACCTGTCCAACGCTGTCAGAGCTTAC
GGCGCCTAAAGTCATCCCCGATCTACTTTCCAGTGACAACGGGTTGATTCTGGTCACCGGCGCCACAGGCAGCGGAAAAT
CCACCACGCTGGCGGCGATGGTGGATCATCTCAATAGCCATTGCGAGGGTCATATTCTTACTCTTGAAGATCCGGTGGAG
TTTATTTATCAAAGCAATCGCTGTCTGATTCAACAGCGAGAAATTGGCCTGCACTGTCCCTCCTTTTCTCAGGCGCTACG
TGCGGCATTACGTGAAGATCCCGACGTCATTTTGCTGGGCGAGTTGCGGGATAGCGAAACGATCCGCCTCGCGCTAACGG
CCGCAGAAACCGGGCATCTGGTATTGGCGACGCTGCATACCCGAGGTGCGTCTCAGGCCATCGAACGGCTGGTGGATACT
TTTCCTGCCGAGGAAAAAGATCCTGTAAGAAACCAGCTGGCGGGGAGCTTGCGCGCGGTACTGGCGCAAAAGCTTGAGCG
CGATGCGCAGGGCGGGCGAGTGGCGCTTTTCGAAATGCTGGTGAACACGCCTGCATCCGCGAATCTCATTCGTGAGGGGA
AAACCTGGCAACTGCCCGGCATCATTCAAACCGGGCAGCAGGCAGGGATGCAAAACTTTGACCAAAGCCTGGCCGAACGC
AAGGCGCAGGGACGGCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Vibrio cholerae strain A1552

40.994

98.773

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393

Multiple sequence alignment