Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SYC_RS10765 Genome accession   NC_006576
Coordinates   2169577..2170665 (+) Length   362 a.a.
NCBI ID   WP_011244333.1    Uniprot ID   A0A0H3K7Y3
Organism   Synechococcus elongatus PCC 6301     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2164577..2175665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SYC_RS10755 (syc2021_c) grpE 2166606..2167229 (-) 624 WP_011378302.1 nucleotide exchange factor GrpE -
  SYC_RS10760 (syc2022_d) pilB/pilB1 2167506..2169506 (+) 2001 WP_011244332.1 GspE/PulE family protein Machinery gene
  SYC_RS10765 (syc2023_d) pilT 2169577..2170665 (+) 1089 WP_011244333.1 type IV pilus twitching motility protein PilT Machinery gene
  SYC_RS10770 (syc2024_d) - 2170690..2171922 (+) 1233 WP_011244334.1 type II secretion system F family protein -
  SYC_RS10775 (syc2025_d) - 2171928..2172482 (+) 555 WP_011244335.1 hypothetical protein -
  SYC_RS10780 (syc2026_c) - 2172447..2173694 (-) 1248 WP_011244336.1 NAD(P)/FAD-dependent oxidoreductase -
  SYC_RS10785 (syc2027_d) dusA 2173721..2174752 (+) 1032 WP_011244337.1 tRNA dihydrouridine(20/20a) synthase DusA -
  SYC_RS13645 (syc2028_d) - 2174849..2175103 (+) 255 WP_011378297.1 helix-hairpin-helix domain-containing protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39865.06 Da        Isoelectric Point: 6.9676

>NTDB_id=24155 SYC_RS10765 WP_011244333.1 2169577..2170665(+) (pilT) [Synechococcus elongatus PCC 6301]
MELMVEDLMEKLVEMGGSDLHLSVGLPPYFRISGKLTPIGDEPMGEEQCQRLIFSMLNNTQRKMLERNWELDCSYGIRGL
ARFRVNVYKDKGTYAACLRALSAKVPVFNDLGLPQVVREMSEKPRGLILVTGPTGSGKTTTLAAVIELINRSRAEHIITV
EDPIEFVYQPAKSIIHQRQLGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPAEKQTQVRVQLSNSLIAVFSQTLVQKRNARPGSFGRVLAQEIMIVTPAISNLIREGKTAQIYSQIQTGGKLGM
QTLEKALADLYKAGEITFEAAASKTSKPDEMQRLIGNVPVAA

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=24155 SYC_RS10765 WP_011244333.1 2169577..2170665(+) (pilT) [Synechococcus elongatus PCC 6301]
ATGGAGCTGATGGTTGAAGACCTGATGGAAAAGCTGGTGGAGATGGGTGGATCTGACCTTCACCTTTCCGTTGGTCTGCC
GCCCTATTTTCGAATTAGTGGCAAACTGACACCGATCGGCGATGAGCCGATGGGCGAAGAACAGTGCCAACGCCTGATTT
TCAGCATGTTGAACAACACGCAACGCAAAATGCTGGAGCGGAACTGGGAGTTAGACTGCTCCTACGGCATCCGTGGTCTA
GCTCGGTTTCGGGTCAACGTCTATAAAGACAAGGGAACCTATGCGGCCTGTTTGCGGGCACTCAGTGCCAAGGTCCCAGT
GTTTAACGATCTCGGCCTGCCACAGGTTGTCCGGGAAATGAGCGAGAAACCGCGAGGGCTGATCCTAGTGACAGGGCCCA
CGGGTTCAGGCAAGACCACAACTTTGGCTGCAGTGATTGAACTAATCAATCGCAGCCGTGCTGAACATATCATCACGGTT
GAAGACCCAATTGAGTTTGTCTATCAACCGGCAAAAAGTATCATCCACCAGCGTCAGCTGGGTGAGGATACCAAGAGTTT
TGCCAACGCGCTGCGAGCTGCCCTGCGGGAAGACCCTGACATTATCCTCGTGGGTGAGATGCGGGACTTGGAAACGATCC
AGTTGGCAATCACTGCTGCAGAGACCGGCCACCTTGTCTTTGGTACCTTACACACCAGCTCTGCAGCCCAAACCGTTGAC
CGGATTATTGACGTTTTCCCGGCTGAAAAACAGACACAGGTGCGGGTGCAACTCTCGAACTCTCTGATTGCAGTTTTTAG
TCAGACCCTAGTACAGAAACGCAATGCTCGTCCGGGCAGTTTTGGCCGAGTCTTGGCTCAGGAAATTATGATTGTGACGC
CGGCAATCTCGAACTTGATTCGGGAAGGGAAAACGGCGCAGATCTATTCCCAAATTCAGACGGGTGGCAAGTTGGGAATG
CAGACGCTCGAAAAAGCGCTGGCAGACCTCTACAAAGCAGGTGAAATTACCTTTGAAGCAGCTGCATCCAAAACGTCGAA
GCCTGACGAGATGCAACGACTCATCGGTAATGTTCCGGTTGCTGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3K7Y3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.026

95.856

0.508
  pilT Legionella pneumophila strain ERS1305867

53.333

95.304

0.508
  pilT Legionella pneumophila strain Lp02

53.333

95.304

0.508
  pilT Acinetobacter baylyi ADP1

53.043

95.304

0.506
  pilT Pseudomonas aeruginosa PAK

53.043

95.304

0.506
  pilT Acinetobacter baumannii strain A118

52.174

95.304

0.497
  pilT Acinetobacter nosocomialis M2

52.174

95.304

0.497
  pilT Acinetobacter baumannii D1279779

52.174

95.304

0.497
  pilT Neisseria gonorrhoeae MS11

49.284

96.409

0.475
  pilT Neisseria meningitidis 8013

49.284

96.409

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.571

96.685

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.118

93.923

0.461
  pilT Vibrio cholerae strain A1552

49.118

93.923

0.461
  pilU Pseudomonas stutzeri DSM 10701

39.714

96.685

0.384

Multiple sequence alignment