Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GK_RS13490 Genome accession   NC_006510
Coordinates   2639018..2640073 (-) Length   351 a.a.
NCBI ID   WP_011232104.1    Uniprot ID   A0A7U9HUV0
Organism   Geobacillus kaustophilus HTA426     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2634018..2645073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GK_RS13460 (GK2622) - 2634267..2634974 (-) 708 WP_011232098.1 hypothetical protein -
  GK_RS13465 (GK2623) - 2634971..2635546 (-) 576 WP_011232099.1 DUF2681 domain-containing protein -
  GK_RS13470 (GK2624) pilM 2635536..2636468 (-) 933 WP_011232100.1 type IV pilus biogenesis protein PilM -
  GK_RS13475 (GK2625) - 2636496..2637242 (-) 747 WP_011232101.1 prepilin peptidase -
  GK_RS13480 (GK2626) - 2637263..2637724 (-) 462 WP_011232102.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GK_RS13485 (GK2627) - 2637820..2639031 (-) 1212 WP_011232103.1 type II secretion system F family protein -
  GK_RS13490 (GK2628) pilT 2639018..2640073 (-) 1056 WP_011232104.1 type IV pilus twitching motility protein PilT Machinery gene
  GK_RS13495 (GK2629) - 2640086..2641750 (-) 1665 WP_011232105.1 GspE/PulE family protein -
  GK_RS13500 (GK2630) - 2641747..2643087 (-) 1341 WP_011232106.1 VanW family protein -
  GK_RS13505 (GK2631) - 2643105..2643875 (-) 771 WP_011232107.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38589.60 Da        Isoelectric Point: 7.1897

>NTDB_id=24051 GK_RS13490 WP_011232104.1 2639018..2640073(-) (pilT) [Geobacillus kaustophilus HTA426]
MKEKLEALLRAAFEYRASDVHLTVGAPPIFRVNGELKAYGQERLNPEDTEQMAKAIIPPPLWQQFQETGELDFSYGIAGV
SRFRINVFKQRSCVSLAVRLIPPRIPTLEELGLPSVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSRHIITL
EDPIEYLHQHGKSIIDQREVGFDTNSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPSAQQAQIRLQLASVLVAVMAQRLFPDGRKRGRTAAMEILINNAAVANLIRNEKEHQIINIMQTGRSVGMQTME
MSVRELVAAGRIDPLAAEAYLTRERGADGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=24051 GK_RS13490 WP_011232104.1 2639018..2640073(-) (pilT) [Geobacillus kaustophilus HTA426]
ATGAAGGAAAAGCTTGAGGCGTTGTTGCGTGCGGCTTTTGAATACCGTGCGTCTGACGTACATTTGACGGTCGGGGCGCC
GCCGATTTTTCGGGTGAATGGGGAACTGAAGGCGTATGGGCAAGAACGGCTGAACCCGGAGGACACGGAACAAATGGCGA
AAGCCATCATTCCGCCGCCGCTTTGGCAGCAGTTTCAAGAAACGGGGGAGCTTGATTTTTCGTACGGTATTGCGGGTGTC
TCCCGCTTCCGGATCAATGTGTTTAAGCAGCGGTCGTGCGTGTCGCTTGCCGTGCGCCTCATCCCGCCGCGCATTCCGAC
ATTAGAAGAGCTCGGCTTGCCAAGCGTGTTAAAGCGCATCGCGGAAAAGCCGCACGGGCTTGTGCTTGTCACCGGGCCGA
CCGGCAGCGGCAAATCGACCACATTGGCGGCGATGATCGACTACATGAATAAAACGATGTCTAGACATATCATTACGCTG
GAAGATCCGATTGAATATTTGCATCAGCACGGAAAATCGATCATCGACCAGCGGGAAGTTGGCTTTGACACGAACAGTTT
TGCCGCCGGATTGCGCGCTGCGCTTCGTCAAGATCCGGATGTGATTTTAGTCGGGGAAATGCGCGATTTGGAAACGATCC
AAACCGCGATCACTGCCGCGGAAACGGGTCATTTAGTGCTTGGCACCCTTCATACGGCGAGCGCCCCAGCGGCGATCGAA
CGGATGATTGACGTCTTTCCGTCAGCCCAGCAGGCGCAAATTCGGCTGCAGCTCGCTTCCGTCCTTGTTGCCGTCATGGC
CCAACGTTTATTTCCCGACGGTCGGAAGAGAGGACGGACGGCGGCTATGGAAATTTTAATCAACAATGCCGCAGTGGCCA
ATTTGATCCGCAATGAAAAAGAGCATCAAATCATCAATATTATGCAGACGGGCCGCAGTGTGGGCATGCAGACGATGGAA
ATGAGCGTCAGGGAGCTTGTGGCCGCTGGCCGTATTGATCCATTGGCCGCGGAAGCGTATTTGACAAGGGAGCGTGGAGC
GGATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U9HUV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.272

89.174

0.493
  pilT Legionella pneumophila strain ERS1305867

48.071

96.011

0.462
  pilT Legionella pneumophila strain Lp02

48.071

96.011

0.462
  pilT Neisseria gonorrhoeae MS11

48.788

94.017

0.459
  pilT Neisseria meningitidis 8013

48.788

94.017

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.936

93.732

0.459
  pilT Vibrio cholerae strain A1552

48.936

93.732

0.459
  pilT Acinetobacter baumannii strain A118

48.485

94.017

0.456
  pilT Acinetobacter baumannii D1279779

48.485

94.017

0.456
  pilT Acinetobacter baylyi ADP1

47.892

94.587

0.453
  pilT Acinetobacter nosocomialis M2

48.182

94.017

0.453
  pilT Pseudomonas aeruginosa PAK

50

90.028

0.45
  pilT Pseudomonas stutzeri DSM 10701

48.418

90.028

0.436
  pilU Pseudomonas stutzeri DSM 10701

43.161

93.732

0.405
  pilU Vibrio cholerae strain A1552

42.857

93.732

0.402

Multiple sequence alignment