Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CAL61_RS18810 Genome accession   NZ_CP021167
Coordinates   3865691..3866671 (-) Length   326 a.a.
NCBI ID   WP_023314823.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 388     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3860691..3871671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL61_RS18780 (CAL61_18670) - 3861568..3862065 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  CAL61_RS18785 (CAL61_18675) endA 3862160..3862867 (+) 708 WP_003860034.1 deoxyribonuclease I -
  CAL61_RS18790 (CAL61_18680) rsmE 3862919..3863650 (+) 732 WP_059370712.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CAL61_RS18795 (CAL61_18685) gshB 3863682..3864629 (+) 948 WP_003860032.1 glutathione synthase -
  CAL61_RS18800 (CAL61_18690) - 3864704..3865264 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  CAL61_RS18805 (CAL61_18695) ruvX 3865264..3865680 (+) 417 WP_003860030.1 Holliday junction resolvase RuvX -
  CAL61_RS18810 (CAL61_18700) pilT 3865691..3866671 (-) 981 WP_023314823.1 type IV pilus twitching motility protein PilT Machinery gene
  CAL61_RS18815 (CAL61_18705) - 3866689..3867387 (+) 699 WP_023314824.1 YggS family pyridoxal phosphate-dependent enzyme -
  CAL61_RS18820 (CAL61_18710) - 3867412..3867978 (+) 567 WP_003860024.1 YggT family protein -
  CAL61_RS18825 (CAL61_18715) yggU 3867975..3868271 (+) 297 WP_110108726.1 DUF167 family protein YggU -
  CAL61_RS18830 (CAL61_18720) - 3868275..3868868 (+) 594 WP_022651831.1 XTP/dITP diphosphatase -
  CAL61_RS18835 (CAL61_18725) hemW 3868861..3870009 (+) 1149 WP_045332868.1 radical SAM family heme chaperone HemW -
  CAL61_RS18840 (CAL61_18730) - 3870066..3870428 (+) 363 WP_110108727.1 DUF559 domain-containing protein -
  CAL61_RS18845 (CAL61_18735) - 3870495..3871211 (-) 717 WP_003862419.1 DUF2884 domain-containing protein -
  CAL61_RS18850 (CAL61_18740) - 3871269..3871595 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35608.58 Da        Isoelectric Point: 6.2335

>NTDB_id=228724 CAL61_RS18810 WP_023314823.1 3865691..3866671(-) (pilT) [Enterobacter hormaechei strain 388]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRV

Nucleotide


Download         Length: 981 bp        

>NTDB_id=228724 CAL61_RS18810 WP_023314823.1 3865691..3866671(-) (pilT) [Enterobacter hormaechei strain 388]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCATTATTAAAAGCGTGGCTCAATG
ACGAACAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGCCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCTGCACAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGCGCTCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAACTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.456

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.456

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

47.72

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria meningitidis 8013

46.97

100

0.475
  pilT Acinetobacter baumannii D1279779

46.809

100

0.472
  pilT Acinetobacter baumannii strain A118

46.809

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.809

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.667

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment