Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MS7163_RS18895 Genome accession   NZ_CP026853
Coordinates   3594213..3595646 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain MS7163     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3589213..3600646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS7163_RS18865 (MS7163_03494) yrbG 3589771..3590748 (+) 978 WP_000922880.1 calcium/sodium antiporter -
  MS7163_RS18870 (MS7163_03495) kdsD 3590762..3591748 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  MS7163_RS18875 (MS7163_03496) kdsC 3591769..3592335 (+) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  MS7163_RS18880 (MS7163_03497) lptC 3592332..3592907 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  MS7163_RS18885 (MS7163_03498) lptA 3592876..3593433 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  MS7163_RS18890 (MS7163_03499) lptB 3593440..3594165 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  MS7163_RS18895 (MS7163_03500) rpoN/rpoN1 3594213..3595646 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  MS7163_RS18900 (MS7163_03501) hpf 3595669..3595956 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  MS7163_RS18905 (MS7163_03502) ptsN 3596074..3596565 (+) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  MS7163_RS18910 (MS7163_03503) rapZ 3596611..3597465 (+) 855 WP_000243741.1 RNase adapter RapZ -
  MS7163_RS18915 (MS7163_03504) npr 3597462..3597734 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  MS7163_RS18920 (MS7163_03505) yrbL 3597948..3598580 (+) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  MS7163_RS18925 (MS7163_03506) mtgA 3598577..3599305 (-) 729 WP_000047069.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  MS7163_RS18930 (MS7163_03507) elbB 3599302..3599955 (-) 654 WP_000719795.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=228724 MS7163_RS18895 WP_000809051.1 3594213..3595646(+) (rpoN/rpoN1) [Escherichia coli strain MS7163]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=228724 MS7163_RS18895 WP_000809051.1 3594213..3595646(+) (rpoN/rpoN1) [Escherichia coli strain MS7163]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCATTTT
CCGACACTGACCGCGCGATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465

Multiple sequence alignment