Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   XcfCFBP6167P_RS06785 Genome accession   NZ_CP021018
Coordinates   1542923..1543363 (+) Length   146 a.a.
NCBI ID   WP_005934296.1    Uniprot ID   -
Organism   Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537923..1548363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XcfCFBP6167P_RS06770 (XcfCFBP6167P_14190) coaE 1539848..1540456 (-) 609 WP_022558665.1 dephospho-CoA kinase -
  XcfCFBP6167P_RS06775 (XcfCFBP6167P_14195) - 1540470..1541333 (-) 864 WP_022558666.1 A24 family peptidase -
  XcfCFBP6167P_RS06780 (XcfCFBP6167P_14200) pilC 1541340..1542596 (-) 1257 WP_007973903.1 type II secretion system F family protein Machinery gene
  XcfCFBP6167P_RS06785 (XcfCFBP6167P_14205) pilE 1542923..1543363 (+) 441 WP_005934296.1 pilin Machinery gene
  XcfCFBP6167P_RS06790 (XcfCFBP6167P_14210) - 1543472..1543873 (+) 402 WP_046121195.1 pilin -
  XcfCFBP6167P_RS06795 (XcfCFBP6167P_14215) pilB 1543936..1545672 (+) 1737 WP_022558667.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XcfCFBP6167P_RS06800 (XcfCFBP6167P_14225) pilR 1546122..1547576 (-) 1455 WP_022558668.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14934.04 Da        Isoelectric Point: 9.0090

>NTDB_id=227891 XcfCFBP6167P_RS06785 WP_005934296.1 1542923..1543363(+) (pilE) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYLARSQVSEGLSLASGAKTAVAETYANTGAFPANNAAAGLETAANIKG
KYITSVTVSSGGVITALFNSSNAKLSGKNLVLTPTDNNGSISWGCSTGTTIDQKYLPTSCRTAATP

Nucleotide


Download         Length: 441 bp        

>NTDB_id=227891 XcfCFBP6167P_RS06785 WP_005934296.1 1542923..1543363(+) (pilE) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167]
ATGAAGAAGCAAAACGGTTTTACGTTGATCGAACTCATGATCGTGGTCGCGATCATTGCGATCCTGGCTGCAATTGCTCT
GCCGGCTTATCAGGACTACCTTGCGCGTTCGCAGGTTTCTGAAGGTCTGTCGCTGGCATCGGGTGCAAAGACCGCAGTTG
CTGAGACGTATGCCAACACTGGTGCATTTCCGGCCAACAACGCAGCGGCCGGTCTTGAGACTGCGGCAAACATCAAGGGT
AAGTACATCACCTCGGTGACCGTTAGCTCTGGTGGCGTCATCACTGCGCTGTTTAATAGCTCCAATGCGAAGCTGAGTGG
TAAGAACCTCGTCCTGACTCCGACGGACAACAACGGGTCGATTAGCTGGGGCTGCAGCACTGGCACCACCATCGATCAGA
AGTATCTGCCTACCTCTTGCCGTACTGCGGCTACCCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

48.75

100

0.534
  pilE Neisseria gonorrhoeae strain FA1090

47.742

100

0.507
  pilA2 Legionella pneumophila str. Paris

50.714

95.89

0.486
  pilA2 Legionella pneumophila strain ERS1305867

50

95.89

0.479
  pilA/pilA1 Eikenella corrodens VA1

45.161

100

0.479
  comP Acinetobacter baylyi ADP1

46

100

0.473
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.87

100

0.452
  pilA Ralstonia pseudosolanacearum GMI1000

38.415

100

0.432
  pilA Acinetobacter baumannii strain A118

42.282

100

0.432
  pilA Vibrio cholerae strain A1552

39.474

100

0.411
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.474

100

0.411
  pilA Vibrio cholerae C6706

39.474

100

0.411
  pilA Pseudomonas aeruginosa PAK

38.816

100

0.404
  pilA Vibrio campbellii strain DS40M4

37.584

100

0.384
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.849

95.205

0.37

Multiple sequence alignment