Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GTHT12_RS13725 Genome accession   NZ_CP020030
Coordinates   2724123..2725178 (-) Length   351 a.a.
NCBI ID   WP_008881087.1    Uniprot ID   A0A0Q0USW4
Organism   Geobacillus thermodenitrificans strain T12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719123..2730178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTHT12_RS13695 (GTHT12_02125) - 2719416..2720084 (-) 669 WP_011887912.1 pilus assembly protein PilO -
  GTHT12_RS13700 (GTHT12_02126) - 2720081..2720656 (-) 576 WP_008881082.1 PilN domain-containing protein -
  GTHT12_RS13705 (GTHT12_02127) pilM 2720646..2721578 (-) 933 WP_008881083.1 pilus assembly protein PilM -
  GTHT12_RS13710 (GTHT12_02128) - 2721604..2722350 (-) 747 WP_011887913.1 A24 family peptidase -
  GTHT12_RS13715 (GTHT12_02129) - 2722356..2722838 (-) 483 WP_172825309.1 type II secretion system protein -
  GTHT12_RS13720 (GTHT12_02130) - 2722925..2724136 (-) 1212 WP_081156941.1 type II secretion system F family protein -
  GTHT12_RS13725 (GTHT12_02131) pilT 2724123..2725178 (-) 1056 WP_008881087.1 type IV pilus twitching motility protein PilT Machinery gene
  GTHT12_RS13730 (GTHT12_02132) - 2725191..2726855 (-) 1665 WP_008881088.1 ATPase, T2SS/T4P/T4SS family -
  GTHT12_RS13735 (GTHT12_02133) - 2726852..2728219 (-) 1368 WP_087960165.1 VanW family protein -
  GTHT12_RS13740 (GTHT12_02134) - 2728589..2729935 (-) 1347 WP_011887916.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38693.73 Da        Isoelectric Point: 7.0292

>NTDB_id=220539 GTHT12_RS13725 WP_008881087.1 2724123..2725178(-) (pilT) [Geobacillus thermodenitrificans strain T12]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRVDGELKAYGHERLRPEETEQMAKAIIPPPLWQQFQETGELDFSYGITGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPAVLKRIAERPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHFVLGTLHTASAPAAIE
RMIDVFPSAQQTQIRLQLASVLVAVIAQRLFPNSRGNGRVAAFEILINNSAVANLIRNEKEHQIVNVMQTSKNVGMQTME
MSIKELVADGRIDPRAAEAYLVGERGEHGTI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=220539 GTHT12_RS13725 WP_008881087.1 2724123..2725178(-) (pilT) [Geobacillus thermodenitrificans strain T12]
ATGAAAGAAAAACTTGAGGCGTTATTACGTGCCGCCTTTGAATGCCGTGCGTCCGATGTGCATTTAACTGTCGGCGCACC
GCCGATTTTTCGTGTAGACGGGGAACTGAAAGCATACGGCCATGAGCGGCTTCGTCCGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCACCGCCGCTTTGGCAGCAGTTTCAAGAAACAGGGGAGCTCGACTTTTCGTACGGCATAACCGGAGTT
TCCCGCTTCCGTTTAAATGTGTTTAAACAGCGGTCGTGTGTTTCTTTAGCTGTGCGCCTCATTCCGATGCGCATCCCGAC
ACTTGAGGAGCTTGGCATGCCGGCCGTCTTAAAACGCATCGCCGAAAGGCCGCATGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGTGGTAAATCGACGACATTGGCAGCCATGATCGACTATATGAACAAAACGATGTCCAAACATATCATTACGCTC
GAAGATCCGATCGAATATTTACATAAACATGGAAAATCGATCATCGACCAGCGTGAAATCGGGTTTGACACCGGCAGCTT
TGCCGCTGGTTTGCGCGCTGCACTTCGCCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGATCACCGCCGCGGAAACGGGGCATTTCGTATTAGGCACGCTGCATACGGCAAGCGCGCCGGCGGCGATCGAG
CGGATGATTGACGTTTTTCCGTCGGCCCAGCAGACGCAAATTCGCTTGCAGCTCGCTTCCGTTCTTGTCGCAGTCATCGC
CCAACGTCTGTTTCCGAATAGCCGGGGGAACGGACGAGTGGCAGCGTTCGAAATTTTAATCAATAATTCCGCGGTCGCCA
ACTTAATTCGTAATGAAAAAGAACATCAAATCGTTAACGTGATGCAAACAAGCAAAAACGTTGGCATGCAGACGATGGAA
ATGAGCATCAAAGAGCTTGTGGCCGACGGGCGCATCGATCCACGTGCAGCCGAGGCGTATTTGGTAGGGGAGCGTGGCGA
ACATGGCACAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0USW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.605

90.883

0.487
  pilT Neisseria gonorrhoeae MS11

49.394

94.017

0.464
  pilT Neisseria meningitidis 8013

49.394

94.017

0.464
  pilT Acinetobacter baylyi ADP1

48.494

94.587

0.459
  pilT Pseudomonas aeruginosa PAK

49.845

92.023

0.459
  pilT Acinetobacter baumannii strain A118

48.485

94.017

0.456
  pilT Acinetobacter baumannii D1279779

48.485

94.017

0.456
  pilT Vibrio cholerae strain A1552

47.904

95.157

0.456
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.904

95.157

0.456
  pilT Acinetobacter nosocomialis M2

48.182

94.017

0.453
  pilT Pseudomonas stutzeri DSM 10701

48.024

93.732

0.45
  pilT Legionella pneumophila strain Lp02

46.667

94.017

0.439
  pilT Legionella pneumophila strain ERS1305867

46.667

94.017

0.439
  pilU Vibrio cholerae strain A1552

42.814

95.157

0.407
  pilU Pseudomonas stutzeri DSM 10701

42.249

93.732

0.396

Multiple sequence alignment