Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B2D07_RS16385 Genome accession   NZ_CP019913
Coordinates   3733238..3734320 (+) Length   360 a.a.
NCBI ID   WP_020877883.1    Uniprot ID   -
Organism   Desulfococcus multivorans strain DSM 2059     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3728238..3739320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D07_RS16355 (B2D07_16505) proC 3729805..3730656 (-) 852 WP_020877878.1 pyrroline-5-carboxylate reductase -
  B2D07_RS16360 (B2D07_16510) - 3731059..3731253 (+) 195 WP_040416134.1 type II toxin-antitoxin system VapB family antitoxin -
  B2D07_RS16365 (B2D07_16515) - 3731256..3731621 (+) 366 WP_020877879.1 PIN domain-containing protein -
  B2D07_RS16370 (B2D07_16520) - 3731726..3732559 (+) 834 WP_020877880.1 hypothetical protein -
  B2D07_RS16375 (B2D07_16525) - 3732559..3732852 (+) 294 WP_040416137.1 hypothetical protein -
  B2D07_RS16380 (B2D07_16530) - 3732882..3733181 (+) 300 WP_020877882.1 YggT family protein -
  B2D07_RS16385 (B2D07_16535) pilT 3733238..3734320 (+) 1083 WP_020877883.1 type IV pilus twitching motility protein PilT Machinery gene
  B2D07_RS16390 (B2D07_16540) - 3734344..3735498 (+) 1155 WP_020877884.1 PilT/PilU family type 4a pilus ATPase -
  B2D07_RS16395 (B2D07_16545) - 3735495..3736172 (+) 678 WP_020877885.1 transcriptional regulator -
  B2D07_RS16400 (B2D07_16550) - 3736156..3737412 (-) 1257 WP_040416141.1 MFS transporter -
  B2D07_RS16405 (B2D07_16555) - 3737430..3738215 (-) 786 WP_020877887.1 type III pantothenate kinase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39805.82 Da        Isoelectric Point: 7.4754

>NTDB_id=218777 B2D07_RS16385 WP_020877883.1 3733238..3734320(+) (pilT) [Desulfococcus multivorans strain DSM 2059]
MARIDAFFKLMNDQGASDLHLVSGQPPALRLRGDIERVKYKILDNDELKAMLYEIAPEDKIKVFEETGDVDFGYEIPGLA
RYRANFFMQKNGCGAVFREIPSTILTVEKLGLPPVIAKLANLPRGLVLVTGPTGSGKSTTLAAIIDEANRTRKDHIITIE
DPIEFVHASQKCIVNHREVGNHTRSFSAALRGALREDPDIILVGELRDLETISLAIEAASTGHLVFGTLHTSSAAKTVDR
AIEVFPAAQQAQIRSTLADGIRAVVAQTLFKRIDIKGRVAALEILIATPAVRNLVRENKTYQINSMIQTGKKYGMRLLDD
AIMELLEKGWISPDDAYNKANDKSRFRPFIKNPPSDFTDV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=218777 B2D07_RS16385 WP_020877883.1 3733238..3734320(+) (pilT) [Desulfococcus multivorans strain DSM 2059]
ATGGCCAGGATCGACGCATTTTTTAAACTGATGAACGACCAGGGGGCCTCGGACCTCCACCTCGTATCGGGCCAGCCCCC
GGCGCTCCGACTCCGCGGGGATATCGAGCGGGTCAAGTACAAGATCCTCGACAACGACGAGCTCAAGGCCATGCTTTACG
AGATCGCCCCCGAGGACAAGATCAAGGTGTTCGAGGAGACCGGGGACGTGGATTTCGGGTACGAGATCCCGGGGCTCGCC
CGTTACCGCGCCAACTTCTTCATGCAGAAGAACGGGTGCGGCGCCGTCTTCCGGGAAATCCCCAGCACGATCCTCACTGT
CGAGAAGCTGGGGCTTCCGCCCGTGATCGCCAAGCTGGCCAACCTGCCCCGGGGGCTCGTCCTCGTCACCGGGCCCACGG
GTTCGGGCAAATCGACCACCCTGGCGGCCATCATCGACGAGGCCAACCGAACCCGCAAGGACCACATCATCACCATCGAG
GATCCCATCGAGTTCGTTCACGCCAGCCAGAAATGCATCGTCAACCACCGGGAGGTGGGCAACCACACCCGAAGCTTCAG
CGCGGCCCTCCGGGGGGCCCTCCGGGAGGACCCCGACATCATCCTCGTGGGCGAGCTTCGGGACCTGGAAACCATCTCCC
TGGCCATCGAGGCCGCCTCCACCGGCCACCTCGTCTTCGGGACCCTCCACACCAGCAGCGCGGCCAAGACCGTCGACCGC
GCCATCGAGGTGTTCCCCGCCGCCCAGCAGGCCCAGATCCGGTCGACCCTGGCCGACGGCATCCGGGCCGTCGTGGCCCA
GACGCTCTTCAAGCGCATCGACATCAAGGGCCGGGTGGCCGCCCTGGAGATTCTCATCGCCACCCCGGCCGTCCGCAACC
TGGTGCGGGAAAACAAGACCTACCAGATCAACTCCATGATCCAGACCGGGAAGAAATACGGCATGCGGCTTCTGGACGAC
GCCATCATGGAGCTGCTCGAAAAAGGCTGGATCAGCCCCGACGACGCCTACAACAAAGCCAACGACAAGTCGCGGTTCCG
GCCCTTCATCAAGAACCCGCCCTCGGATTTCACCGACGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

57.958

92.5

0.536
  pilT Pseudomonas aeruginosa PAK

57.057

92.5

0.528
  pilT Legionella pneumophila strain Lp02

55.891

91.944

0.514
  pilT Legionella pneumophila strain ERS1305867

55.891

91.944

0.514
  pilT Acinetobacter nosocomialis M2

53.916

92.222

0.497
  pilT Acinetobacter baumannii D1279779

53.916

92.222

0.497
  pilT Acinetobacter baumannii strain A118

53.916

92.222

0.497
  pilT Acinetobacter baylyi ADP1

55.521

88.056

0.489
  pilT Neisseria meningitidis 8013

53.517

90.833

0.486
  pilT Neisseria gonorrhoeae MS11

53.211

90.833

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.271

95.278

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.84

90.556

0.469
  pilT Vibrio cholerae strain A1552

51.84

90.556

0.469
  pilU Pseudomonas stutzeri DSM 10701

39.481

96.389

0.381
  pilU Acinetobacter baylyi ADP1

37.64

98.889

0.372

Multiple sequence alignment