Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BUE86_RS18390 Genome accession   NZ_CP018785
Coordinates   3803393..3804418 (+) Length   341 a.a.
NCBI ID   WP_079231689.1    Uniprot ID   -
Organism   Enterobacter ludwigii strain AA4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3798393..3809418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUE86_RS18350 - 3798474..3798800 (+) 327 WP_032678951.1 YggL family protein -
  BUE86_RS18355 - 3798857..3799573 (+) 717 WP_020883689.1 DUF2884 domain-containing protein -
  BUE86_RS18360 - 3799635..3799997 (-) 363 WP_020883690.1 endonuclease domain-containing protein -
  BUE86_RS18365 hemW 3800061..3801203 (-) 1143 WP_020883691.1 radical SAM family heme chaperone HemW -
  BUE86_RS18370 - 3801196..3801789 (-) 594 WP_050737284.1 XTP/dITP diphosphatase -
  BUE86_RS18375 yggU 3801793..3802089 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  BUE86_RS18380 - 3802086..3802652 (-) 567 WP_014171570.1 YggT family protein -
  BUE86_RS18385 - 3802674..3803375 (-) 702 WP_079231688.1 YggS family pyridoxal phosphate-dependent enzyme -
  BUE86_RS18390 pilT 3803393..3804418 (+) 1026 WP_079231689.1 type IV pilus twitching motility protein PilT Machinery gene
  BUE86_RS18395 ruvX 3804384..3804800 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  BUE86_RS18400 - 3804800..3805360 (-) 561 WP_020883695.1 YqgE/AlgH family protein -
  BUE86_RS18405 gshB 3805447..3806394 (-) 948 WP_020883696.1 glutathione synthase -
  BUE86_RS18410 rsmE 3806434..3807165 (-) 732 WP_079231690.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  BUE86_RS18415 endA 3807218..3807925 (-) 708 WP_020883698.1 deoxyribonuclease I -
  BUE86_RS18420 - 3808020..3808517 (-) 498 WP_047956084.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37122.19 Da        Isoelectric Point: 6.2326

>NTDB_id=210171 BUE86_RS18390 WP_079231689.1 3803393..3804418(+) (pilT) [Enterobacter ludwigii strain AA4]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPPPEVDTLLKAWLNDEQQGTWWANGQVDFAVTLAGNQRLRG
SAFKQTSGVSMTLRLLPGVCPQLPALGVPRVVPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFADALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDAQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGHQAGMQNFDQSLAER
RAQGQLQAPALNNPVRNSFRE

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=210171 BUE86_RS18390 WP_079231689.1 3803393..3804418(+) (pilT) [Enterobacter ludwigii strain AA4]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGAGCAGGTCGTCTTGAACCCGCGCCGTTTCCGCCTCCCGAAGTCGATACGCTATTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGAACGTGGTGGGCGAACGGGCAGGTGGATTTTGCCGTTACCCTGGCGGGCAATCAGCGCCTGCGCGGA
AGCGCGTTTAAACAGACAAGCGGCGTCTCAATGACGCTGCGGCTGTTGCCCGGGGTCTGCCCGCAACTCCCCGCGTTGGG
GGTGCCCCGGGTTGTTCCTGAGCTGTTATCCAGTGACAACGGGTTGATCCTGGTCACCGGGGCAACCGGCAGCGGTAAGT
CCACTACGCTGGCAGCGATGGTAGATTTTCTTAATCACCATACTGACGGGCATATTCTGACGCTCGAAGACCCGGTGGAG
TTTCTGTACCAGAGCGAACGGTGCCTGATCCAGCAGCGCGAGATTGGCCAACACAGCCCGTCATTTGCCGACGCGCTGCG
CGGGGCGTTACGCCAGGATCCTGATGTCATACTACTGGGTGAGCTGCGCGACAGTGAAACAATCCGCCTCGCGCTGACGG
CGGCAGAAACCGGACATCTGGTGCTCGCGACACTGCATACCCGCGGGGCAGCGCAGGCGATTGAGCGGCTGGTCGATACG
TTTCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTCCTCCC
CGATGCGCAGGGCGGGCGCGTGGCGCTTTATGAGCTGCTGGTGAATACTCCGGCGGCGGCGAATCTCATTCGCGAAGGAA
AAACGTGGCAATTGCCCGGTATTATTCAAACCGGCCACCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCTGAAAGA
CGGGCGCAGGGGCAACTCCAGGCCCCGGCGCTCAATAACCCTGTTCGAAATAGCTTTCGAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.112

99.12

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

99.12

0.487
  pilT Neisseria meningitidis 8013

48.048

97.654

0.469
  pilT Neisseria gonorrhoeae MS11

46.491

100

0.466
  pilT Legionella pneumophila strain ERS1305867

48.758

94.428

0.46
  pilT Legionella pneumophila strain Lp02

48.758

94.428

0.46
  pilT Acinetobacter baylyi ADP1

46.988

97.361

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.706

95.894

0.457
  pilT Acinetobacter baumannii D1279779

47.401

95.894

0.455
  pilT Pseudomonas aeruginosa PAK

47.401

95.894

0.455
  pilT Acinetobacter baumannii strain A118

47.401

95.894

0.455
  pilT Acinetobacter nosocomialis M2

47.812

93.842

0.449
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.41

94.428

0.419
  pilU Pseudomonas stutzeri DSM 10701

40.303

96.774

0.39
  pilU Vibrio cholerae strain A1552

39.13

94.428

0.369

Multiple sequence alignment