Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BJD12_RS06960 Genome accession   NZ_CP018725
Coordinates   1607857..1608267 (-) Length   136 a.a.
NCBI ID   WP_005992828.1    Uniprot ID   F0BE35
Organism   Xanthomonas vesicatoria ATCC 35937 strain LMG911     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1602857..1613267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD12_RS24145 - 1603632..1603778 (+) 147 WP_005992831.1 hypothetical protein -
  BJD12_RS06945 (BJD12_06945) pilR 1603876..1605270 (+) 1395 WP_005992830.1 sigma-54 dependent transcriptional regulator Regulator
  BJD12_RS06950 (BJD12_06950) pilB 1605461..1607194 (-) 1734 WP_042828050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJD12_RS06955 (BJD12_06955) - 1607257..1607664 (-) 408 WP_042828048.1 pilin -
  BJD12_RS06960 (BJD12_06960) pilA 1607857..1608267 (-) 411 WP_005992828.1 pilin Machinery gene
  BJD12_RS06965 (BJD12_06965) pilC 1608606..1609862 (+) 1257 WP_058562594.1 type II secretion system F family protein Machinery gene
  BJD12_RS06970 (BJD12_06970) - 1609869..1610732 (+) 864 WP_005992825.1 A24 family peptidase -
  BJD12_RS06975 (BJD12_06975) coaE 1610746..1611351 (+) 606 WP_005992824.1 dephospho-CoA kinase -
  BJD12_RS06980 (BJD12_06980) - 1611424..1612758 (-) 1335 WP_005992823.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14301.72 Da        Isoelectric Point: 8.4764

>NTDB_id=209875 BJD12_RS06960 WP_005992828.1 1607857..1608267(-) (pilA) [Xanthomonas vesicatoria ATCC 35937 strain LMG911]
MKKQNGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVTAGLAEINPGKTQYEVALNEGKTTVADITELGLKSPSERCT
IAPITALSATGTIECTLKGNTQVVGKKVTLTRANDGTWTCKTDALKKYAPAGCPGA

Nucleotide


Download         Length: 411 bp        

>NTDB_id=209875 BJD12_RS06960 WP_005992828.1 1607857..1608267(-) (pilA) [Xanthomonas vesicatoria ATCC 35937 strain LMG911]
ATGAAGAAGCAAAACGGTTTTACGCTGATCGAACTGATGATCGTGATTGCAATCATCGCAATTCTGGCTGCTATTGCACT
GCCGATGTATCAAGACTATGTCGCCAAGTCGCAGGTCACCGCCGGCCTCGCTGAGATCAATCCTGGCAAGACGCAGTACG
AAGTGGCACTTAACGAAGGCAAAACCACCGTTGCCGACATCACCGAGTTGGGTCTGAAGTCTCCCTCTGAGCGCTGCACC
ATTGCGCCGATCACCGCTCTGAGCGCTACTGGCACCATCGAGTGTACTCTGAAGGGCAATACGCAGGTTGTGGGCAAGAA
GGTGACATTGACGCGTGCAAATGATGGTACCTGGACCTGCAAGACGGATGCGCTGAAGAAGTACGCGCCTGCCGGTTGCC
CGGGTGCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0BE35

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.365

100

0.507
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.111

99.265

0.507
  pilA Pseudomonas aeruginosa PAK

43.243

100

0.471
  pilA Vibrio cholerae strain A1552

42.553

100

0.441
  pilA Vibrio cholerae C6706

42.553

100

0.441
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.553

100

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.284

98.529

0.426
  comP Acinetobacter baylyi ADP1

38.926

100

0.426
  pilA2 Legionella pneumophila str. Paris

41.912

100

0.419
  pilA2 Legionella pneumophila strain ERS1305867

40.441

100

0.404
  pilE Neisseria gonorrhoeae strain FA1090

38.931

96.324

0.375
  pilE Neisseria gonorrhoeae MS11

39.2

91.912

0.36

Multiple sequence alignment