Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJD12_RS06950 Genome accession   NZ_CP018725
Coordinates   1605461..1607194 (-) Length   577 a.a.
NCBI ID   WP_042828050.1    Uniprot ID   -
Organism   Xanthomonas vesicatoria ATCC 35937 strain LMG911     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1600461..1612194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJD12_RS06935 (BJD12_06935) sucC 1600502..1601671 (-) 1170 WP_005992833.1 ADP-forming succinate--CoA ligase subunit beta -
  BJD12_RS06940 (BJD12_06940) - 1601903..1603516 (+) 1614 WP_005992832.1 HAMP domain-containing sensor histidine kinase -
  BJD12_RS24145 - 1603632..1603778 (+) 147 WP_005992831.1 hypothetical protein -
  BJD12_RS06945 (BJD12_06945) pilR 1603876..1605270 (+) 1395 WP_005992830.1 sigma-54 dependent transcriptional regulator Regulator
  BJD12_RS06950 (BJD12_06950) pilB 1605461..1607194 (-) 1734 WP_042828050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJD12_RS06955 (BJD12_06955) - 1607257..1607664 (-) 408 WP_042828048.1 pilin -
  BJD12_RS06960 (BJD12_06960) pilA 1607857..1608267 (-) 411 WP_005992828.1 pilin Machinery gene
  BJD12_RS06965 (BJD12_06965) pilC 1608606..1609862 (+) 1257 WP_058562594.1 type II secretion system F family protein Machinery gene
  BJD12_RS06970 (BJD12_06970) - 1609869..1610732 (+) 864 WP_005992825.1 A24 family peptidase -
  BJD12_RS06975 (BJD12_06975) coaE 1610746..1611351 (+) 606 WP_005992824.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62712.87 Da        Isoelectric Point: 5.6021

>NTDB_id=209874 BJD12_RS06950 WP_042828050.1 1605461..1607194(-) (pilB) [Xanthomonas vesicatoria ATCC 35937 strain LMG911]
MSAVSSANLVGITGIARRLVQDGALEETVARSAMDQAAAAKVPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGASLG
NDDDDMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLDGGNAMQIAEAAQKIGIRDLRQSALMK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=209874 BJD12_RS06950 WP_042828050.1 1605461..1607194(-) (pilB) [Xanthomonas vesicatoria ATCC 35937 strain LMG911]
ATGAGTGCTGTTTCGTCCGCTAATTTAGTTGGTATTACCGGAATCGCGCGTCGTTTAGTACAAGATGGTGCGCTTGAAGA
GACTGTAGCTCGCTCTGCGATGGATCAGGCAGCAGCGGCTAAGGTTCCATTACCTCAATGGTTTTCAGAAAAAAAGTTGG
TGTCGGCCTCCCAATTGGCTGCTGCCAACGCGGTCGAATTTGGCATGCCGTTGATGGATGTGTCGGTGTTCGACGCCAAC
CAGAACGCGGTCAAACTGGTCAGTGAAGAGTTGCTCCAGAAGTATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGA
TCCTGGTTGATGAGGACCAGATCCGTCGCACGCTGGAGCAATGGCAGGCCAGTAACGCCGCGCTAGGCGCGTCGCTCGGC
AACGACGACGACGATATGGGGGACCTAGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGACTCCGGGGTCGA
TGCGAAGGGTGATGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCTGCACGTCTGAAGGTGATGTCGCAACTAGACATCGCCGAGAAGCGAGTGCCGCAGGA
CGGGCGCATCAAGCTCAACTTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGTACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATTCTGGATGCAAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTTTTGGACGCGATCCACAAGCCGTACGGCATGGTGTTGGTGACGGGGCCAACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAATGATGAGACGCGCAATATTTCAACTGCCGAGGATCCGGTCGAAATTCGGTTGCCCG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACCTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCATACCAATGATGCGCCCCAGACCATCGCGCGTTTGATGAACATGGGGATTGCGCCGTACAACATCA
CCTCGTCGGTGACGCTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACCTTGCCG
GAGCATGCGCTGCTGGCAGAAGGGTTTACGCCAGAGCAAGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACCGAAGGCTACAAGGGCCGTACAGGCATTTATCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGG
ACGGCGGCAATGCCATGCAGATCGCCGAGGCTGCGCAAAAGATCGGTATCCGCGACCTGCGCCAGTCGGCATTGATGAAG
GCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACGAAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.922

100

0.551
  pilB Acinetobacter baumannii D1279779

54.75

100

0.549
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilB Vibrio cholerae strain A1552

48.606

99.48

0.484
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.512

96.88

0.451

Multiple sequence alignment