Detailed information NTG0205733

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: Cabys_RS11995
• Genome accession: NZ_CP018099
• Coordinates: 3073476..3074507 (-)
• Length: 343 a.a.
• NCBI ID: WP_006930776.1
• Uniprot ID: H1XYG0
• Organism: Caldithrix abyssi DSM 13497 strain LF13
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3068476..3079507

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Cabys_RS11990 (Cabys_2572)	-	3072062..3073465 (-)	1404	WP_006930775.1	hypothetical protein	-
Cabys_RS11995 (Cabys_2573)	pilT	3073476..3074507 (-)	1032	WP_006930776.1	type IV pilus twitching motility protein PilT	Machinery gene
Cabys_RS12000 (Cabys_2574)	-	3074516..3075817 (-)	1302	WP_006930778.1	secretin and TonB N-terminal domain-containing protein	-
Cabys_RS12005 (Cabys_2575)	-	3075831..3076301 (-)	471	WP_006930784.1	hypothetical protein	-
Cabys_RS12010 (Cabys_2576)	pilO	3076303..3076842 (-)	540	WP_006930785.1	type 4a pilus biogenesis protein PilO	-
Cabys_RS12015 (Cabys_2577)	-	3076854..3078674 (-)	1821	WP_006930786.1	hypothetical protein	-

Sequence

Protein

• Length: 343 a.a.
• Molecular weight: 38716.75 Da
• Isoelectric Point: 9.6856

>NTDB_id=205733 Cabys_RS11995 WP_006930776.1 3073476..3074507(-) (pilT) [Caldithrix abyssi DSM 13497 strain LF13]
MSVNFGQVLRFAVRQNASDIHFSSGMQPIIRLVGELKRIDAPVITYEELKNFIFAYLRPEQKAAFEKNLEIDFSLSIEGI
SRFRVNVFKHSNGLSASLRIIPEQIRTLNELLMPPVLKEIVRHRKGLILVTGPTGSGKSTTLAAMIDEINRNRRDHIITL
EDPIEYLHRPKLSLIHQREVGVHTQSFAQGLRAALREDPDVILVGEMRDIETITNALHAAETGHLVFSTLHTNSASETVD
RIINVFPGEQQQQIRVILAGSLVAVISQRLVRQAHRADRIALLEILIATPAIRNLIREGKTHQIESAIQMGAEYGMRTFQ
KSLDELRQRNLVPPDMIKVDFAN

Nucleotide

• Length: 1032 bp

>NTDB_id=205733 Cabys_RS11995 WP_006930776.1 3073476..3074507(-) (pilT) [Caldithrix abyssi DSM 13497 strain LF13]
ATGTCTGTTAATTTTGGTCAGGTTTTACGTTTTGCAGTAAGGCAAAATGCCTCAGATATTCATTTCAGCAGCGGCATGCA
GCCGATCATTCGTCTGGTCGGCGAGTTGAAAAGAATCGATGCGCCTGTGATAACGTACGAGGAGTTGAAAAACTTTATCT
TCGCTTACTTACGCCCTGAGCAAAAAGCCGCATTTGAAAAAAATCTGGAAATCGATTTTTCATTATCCATCGAGGGGATA
AGTCGATTTCGTGTAAATGTGTTTAAACATTCCAATGGCCTTAGCGCCTCGCTCAGGATCATCCCTGAACAAATTCGCAC
GCTTAACGAACTATTGATGCCGCCGGTATTAAAGGAAATCGTCCGGCATCGAAAAGGTTTGATTCTGGTCACCGGTCCAA
CAGGATCCGGAAAATCGACCACGCTGGCTGCGATGATCGATGAAATAAATCGGAACCGGCGGGACCACATTATTACCCTC
GAAGATCCAATCGAATACCTCCATCGCCCAAAGCTTTCGCTCATTCACCAACGAGAAGTAGGCGTGCACACGCAAAGCTT
TGCGCAGGGGCTGCGCGCTGCCTTACGCGAGGACCCGGATGTTATTCTAGTAGGCGAAATGCGCGATATCGAAACGATTA
CCAACGCCCTGCACGCAGCCGAAACAGGTCATCTGGTTTTTTCGACGTTGCACACCAACTCGGCCAGCGAAACGGTCGAT
CGCATCATTAATGTCTTTCCCGGCGAGCAGCAACAGCAAATTCGCGTTATTCTGGCCGGGTCGCTTGTAGCCGTCATCTC
ACAGCGCCTGGTACGCCAGGCGCACCGGGCAGATCGGATCGCTCTGTTAGAAATCTTAATTGCCACCCCGGCCATTCGAA
ATTTGATTCGGGAGGGGAAAACGCATCAAATAGAATCGGCCATTCAAATGGGGGCAGAGTACGGCATGCGTACCTTTCAG
AAAAGCCTGGATGAGCTGCGTCAAAGAAATTTGGTCCCGCCCGATATGATAAAAGTTGACTTTGCAAATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-273)

3D structure

• AlphaFold DB: H1XYG0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Acinetobacter baylyi ADP1	52.121	96.21	0.501
pilT	Neisseria meningitidis 8013	50.755	96.501	0.49
pilT	Neisseria gonorrhoeae MS11	50.915	95.627	0.487
pilT	Acinetobacter nosocomialis M2	50.301	96.793	0.487
pilT	Acinetobacter baumannii D1279779	50.301	96.793	0.487
pilT	Acinetobacter baumannii strain A118	50.301	96.793	0.487
pilT	Vibrio cholerae strain A1552	50	96.21	0.481
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	50	96.21	0.481
pilT	Pseudomonas stutzeri DSM 10701	48.81	97.959	0.478
pilT	Legionella pneumophila strain Lp02	49.091	96.21	0.472
pilT	Pseudomonas aeruginosa PAK	49.091	96.21	0.472
pilT	Legionella pneumophila strain ERS1305867	49.091	96.21	0.472
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	48.503	97.376	0.472
pilU	Pseudomonas stutzeri DSM 10701	38.889	94.461	0.367
pilU	Acinetobacter baylyi ADP1	38.154	94.752	0.362