Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BM449_RS07840 Genome accession   NZ_CP018091
Coordinates   1498991..1500070 (+) Length   359 a.a.
NCBI ID   WP_071800762.1    Uniprot ID   A0A1J0PAA7
Organism   Synechococcus sp. SynAce01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1493991..1505070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM449_RS07820 (BM449_07550) - 1494780..1495094 (-) 315 WP_071800759.1 sulfurtransferase TusA family protein -
  BM449_RS07825 (BM449_07555) dnaJ 1495115..1496224 (-) 1110 WP_071800760.1 molecular chaperone DnaJ -
  BM449_RS07830 (BM449_07560) grpE 1496224..1496973 (-) 750 WP_071800761.1 nucleotide exchange factor GrpE -
  BM449_RS07835 (BM449_07565) - 1497072..1498874 (+) 1803 WP_120352773.1 GspE/PulE family protein -
  BM449_RS07840 (BM449_07570) pilT 1498991..1500070 (+) 1080 WP_071800762.1 type IV pilus twitching motility protein PilT Machinery gene
  BM449_RS07845 (BM449_07575) - 1500081..1501334 (+) 1254 WP_071800763.1 type II secretion system F family protein -
  BM449_RS07850 (BM449_07580) - 1501327..1502652 (-) 1326 WP_071800764.1 NAD(P)/FAD-dependent oxidoreductase -
  BM449_RS07855 (BM449_07585) dusA 1502659..1503663 (+) 1005 WP_071800765.1 tRNA dihydrouridine(20/20a) synthase DusA -
  BM449_RS07860 (BM449_07590) - 1503775..1504029 (-) 255 Protein_1566 RNA-binding protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39263.45 Da        Isoelectric Point: 5.6341

>NTDB_id=205686 BM449_RS07840 WP_071800762.1 1498991..1500070(+) (pilT) [Synechococcus sp. SynAce01]
MELQIQDLMGQLIQEKGSDLHLSAGQPPFGRFNGELRVMQAARLSEDDCNRMIFSILNDGQRQHLEQTWELDCAYGLRDI
ARFRVNVYSQNGSYAACLRALSSKIPAAGELGLPAVVIETTKRPSGLVLVTGPTGSGKSTTLASLLDEINHTSSKHILTI
EDPIEFVYQSDRSLIHQRQLNEDTKSFSNALRAALREDPDVILVGELRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPSNQQTQVRVQLSGSLAAIFAQTLCLRSDPSGHAFGRVMAQEIMVNTPAVANLIREGKTAQIYSQLQTGGQFGM
QTLEKALAALVAEGAISFDEARSRSTRPDELSRLISSQR

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=205686 BM449_RS07840 WP_071800762.1 1498991..1500070(+) (pilT) [Synechococcus sp. SynAce01]
ATGGAGCTGCAGATTCAAGACTTGATGGGGCAGCTGATCCAGGAGAAAGGCAGCGATCTCCATCTCAGTGCTGGCCAGCC
CCCCTTCGGCCGCTTCAATGGGGAACTGCGGGTCATGCAGGCCGCCAGGCTCTCAGAGGACGACTGCAATCGCATGATCT
TCTCGATTCTCAACGATGGGCAACGCCAGCACCTAGAGCAGACCTGGGAGCTCGATTGCGCCTATGGCCTTCGTGATATC
GCCAGATTTCGCGTCAATGTCTATAGCCAGAATGGGTCCTACGCTGCTTGCCTTCGTGCTCTGAGCAGCAAGATCCCGGC
TGCTGGGGAGCTGGGACTGCCTGCCGTTGTGATCGAGACAACAAAACGACCTTCCGGGCTTGTGCTGGTCACAGGGCCGA
CCGGCTCCGGCAAGAGCACCACCCTGGCTTCGCTGCTTGATGAGATCAATCACACGAGTTCTAAGCATATTCTGACGATT
GAAGATCCGATTGAGTTCGTCTATCAGAGTGATCGCAGCTTGATTCATCAGCGCCAGCTCAACGAAGACACCAAGAGCTT
CAGCAATGCCCTCAGAGCAGCGTTGCGTGAAGATCCCGACGTGATCCTGGTGGGGGAACTGCGCGACCTCGAAACGATTC
AGTTGGCGATCACGGCCGCCGAAACCGGTCATCTCGTTTTCGCCACGCTCCACACCAGCTCCGCCGCCCAGACGGTGGAT
CGGATGGTCGACGTGTTTCCCTCCAACCAGCAGACCCAGGTACGGGTCCAGCTCAGCGGCAGCCTCGCGGCGATCTTTGC
CCAGACCCTCTGCCTGCGCAGCGACCCCAGTGGCCATGCGTTCGGCCGGGTGATGGCCCAGGAAATCATGGTCAACACAC
CGGCTGTAGCCAACCTGATCCGGGAAGGCAAAACAGCTCAGATCTATTCCCAGCTCCAGACCGGCGGCCAGTTCGGCATG
CAGACCCTGGAGAAGGCCCTCGCCGCGTTGGTCGCCGAGGGTGCCATCAGCTTCGATGAGGCCCGCTCCCGATCCACCAG
GCCCGACGAGCTCAGCCGCCTGATCAGCAGCCAGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0PAA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.576

97.214

0.501
  pilT Pseudomonas stutzeri DSM 10701

51.003

97.214

0.496
  pilT Acinetobacter baumannii strain A118

50.716

97.214

0.493
  pilT Acinetobacter baumannii D1279779

50.716

97.214

0.493
  pilT Neisseria meningitidis 8013

50.571

97.493

0.493
  pilT Neisseria gonorrhoeae MS11

50.286

97.493

0.49
  pilT Acinetobacter nosocomialis M2

50.43

97.214

0.49
  pilT Acinetobacter baylyi ADP1

50.432

96.657

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.292

98.329

0.485
  pilT Vibrio cholerae strain A1552

50.877

95.265

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.877

95.265

0.485
  pilT Legionella pneumophila strain ERS1305867

51.642

93.315

0.482
  pilT Legionella pneumophila strain Lp02

51.642

93.315

0.482
  pilU Vibrio cholerae strain A1552

36.695

99.443

0.365
  pilU Pseudomonas stutzeri DSM 10701

36.723

98.607

0.362

Multiple sequence alignment