Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJP61_RS04740 Genome accession   NZ_CP017662
Coordinates   1005160..1006176 (+) Length   338 a.a.
NCBI ID   WP_171997909.1    Uniprot ID   -
Organism   Cronobacter sp. JZ38     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1000160..1011176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJP61_RS04710 ftsP 1000251..1001663 (+) 1413 WP_038867900.1 cell division protein FtsP -
  BJP61_RS04715 hemW 1001836..1002972 (-) 1137 WP_038867902.1 radical SAM family heme chaperone HemW -
  BJP61_RS04720 - 1002965..1003558 (-) 594 WP_171997907.1 XTP/dITP diphosphatase -
  BJP61_RS04725 yggU 1003574..1003864 (-) 291 WP_171997908.1 DUF167 family protein YggU -
  BJP61_RS04730 - 1003861..1004415 (-) 555 WP_038867909.1 YggT family protein -
  BJP61_RS04735 - 1004431..1005144 (-) 714 WP_075193405.1 YggS family pyridoxal phosphate-dependent enzyme -
  BJP61_RS04740 pilT 1005160..1006176 (+) 1017 WP_171997909.1 type IV pilus twitching motility protein PilT Machinery gene
  BJP61_RS04745 - 1006173..1008431 (-) 2259 WP_171997910.1 FAD-dependent oxidoreductase -
  BJP61_RS04750 - 1008494..1009186 (+) 693 WP_075193407.1 IclR family transcriptional regulator -
  BJP61_RS04755 ruvX 1009341..1009757 (-) 417 WP_038867918.1 Holliday junction resolvase RuvX -
  BJP61_RS04760 - 1009757..1010320 (-) 564 WP_038867921.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36673.14 Da        Isoelectric Point: 6.6826

>NTDB_id=201262 BJP61_RS04740 WP_171997909.1 1005160..1006176(+) (pilT) [Cronobacter sp. JZ38]
MEMDELVALSVKHNVSDLHLCSELPARWRRQGRLEPLTHPVPKPENLLARWLDDAHQRELSERGQTDFAVSLAGGVRLCG
NAFRQMQGVSLVLRLLPRGCPRLEALGVPAAVPGLLNMPDGLLLVTGATGSGKSTTLAAMVGYLNETLDGHILTLEDPVE
FCHQPARCLIQQREIGTHCQSFSDGLRGALREDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPADEQNKVRSQLAGSLKAVLAQKLVADAQGGRTALHELLINTPAVANLIREAKTHQLPGLMETGQQHGMQTFAQSVAMR
LNEGRLALQKEASAAHWC

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=201262 BJP61_RS04740 WP_171997909.1 1005160..1006176(+) (pilT) [Cronobacter sp. JZ38]
ATGGAAATGGATGAATTAGTGGCGCTTAGTGTAAAGCATAATGTTTCGGATCTACACCTGTGTAGCGAGCTGCCTGCGCG
CTGGCGCAGGCAAGGGCGGCTGGAGCCGCTTACGCACCCGGTGCCGAAGCCGGAAAACTTGCTGGCGCGCTGGCTCGACG
ACGCCCATCAGCGCGAGCTTTCCGAGCGCGGGCAGACGGATTTCGCGGTGAGTCTGGCAGGCGGCGTGCGCCTTTGCGGG
AACGCGTTTCGCCAGATGCAGGGCGTGTCGCTGGTCTTGCGCCTGCTGCCGCGTGGCTGTCCGCGGCTCGAGGCGCTGGG
CGTACCGGCGGCGGTGCCGGGGCTGCTCAATATGCCGGACGGCCTGCTGCTGGTCACCGGCGCGACCGGCAGCGGTAAAT
CCACCACGCTTGCGGCGATGGTCGGCTACCTGAACGAGACGCTCGACGGCCATATCCTGACGCTTGAAGATCCGGTTGAG
TTTTGTCATCAACCCGCGCGCTGTCTCATTCAGCAGCGTGAAATCGGCACCCACTGTCAGAGCTTCAGCGACGGGCTGCG
TGGCGCGCTACGTGAAGATCCGGATGTCATTTTGCTGGGCGAATTGCGGGACAGCGAAACCATCCGGCTGGCGCTGACGG
CGGCGGAAACCGGCCACCTGGTAATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCCGTCGAGCGCCTGGTGGATGTC
TTTCCGGCGGATGAACAGAACAAAGTACGCAGCCAGCTTGCGGGTAGCCTCAAGGCGGTGCTGGCGCAAAAGCTGGTGGC
GGACGCGCAGGGCGGAAGGACGGCGCTTCATGAACTGCTGATTAACACGCCAGCGGTGGCGAACCTCATCCGTGAGGCGA
AAACGCACCAGTTGCCAGGCCTGATGGAAACCGGCCAGCAGCACGGGATGCAGACGTTCGCGCAAAGTGTCGCGATGCGT
CTTAACGAAGGCAGACTGGCGCTGCAAAAAGAGGCCAGCGCCGCGCACTGGTGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.512

99.408

0.482
  pilT Vibrio cholerae strain A1552

48.512

99.408

0.482
  pilT Neisseria meningitidis 8013

48.494

98.225

0.476
  pilT Neisseria gonorrhoeae MS11

48.193

98.225

0.473
  pilT Acinetobacter baumannii D1279779

45.858

100

0.459
  pilT Acinetobacter baumannii strain A118

45.858

100

0.459
  pilT Acinetobacter nosocomialis M2

45.858

100

0.459
  pilT Pseudomonas aeruginosa PAK

48.742

94.083

0.459
  pilT Acinetobacter baylyi ADP1

46.526

97.929

0.456
  pilT Pseudomonas stutzeri DSM 10701

48.734

93.491

0.456
  pilT Legionella pneumophila strain Lp02

45.758

97.633

0.447
  pilT Legionella pneumophila strain ERS1305867

45.758

97.633

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.519

95.858

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.841

98.521

0.402
  pilU Vibrio cholerae strain A1552

40.12

98.817

0.396

Multiple sequence alignment