Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BJP34_RS27990 Genome accession   NZ_CP017599
Coordinates   7623080..7624198 (+) Length   372 a.a.
NCBI ID   WP_070395171.1    Uniprot ID   A0A1D8TYP6
Organism   Moorena producens PAL-8-15-08-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7618080..7629198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJP34_RS27975 (BJP34_27980) grpE 7618447..7619244 (-) 798 WP_070395168.1 nucleotide exchange factor GrpE -
  BJP34_RS27980 (BJP34_27985) - 7619322..7620539 (-) 1218 WP_070395169.1 RNA-guided endonuclease InsQ/TnpB family protein -
  BJP34_RS27985 (BJP34_27990) pilB/pilB1 7620975..7622981 (+) 2007 WP_070395170.1 GspE/PulE family protein Machinery gene
  BJP34_RS27990 (BJP34_27995) pilT 7623080..7624198 (+) 1119 WP_070395171.1 type IV pilus twitching motility protein PilT Machinery gene
  BJP34_RS27995 (BJP34_28000) - 7624592..7625809 (+) 1218 WP_070395172.1 type II secretion system F family protein -
  BJP34_RS28000 (BJP34_28005) - 7625814..7626461 (+) 648 WP_070395173.1 hypothetical protein -
  BJP34_RS41150 - 7626500..7626697 (+) 198 WP_149031226.1 hypothetical protein -
  BJP34_RS28005 (BJP34_28010) bioU 7626675..7627715 (-) 1041 WP_070395174.1 (S)-8-amino-7-oxononanoate synthase BioU -
  BJP34_RS49590 - 7628151..7628273 (-) 123 WP_267876402.1 hypothetical protein -
  BJP34_RS28010 (BJP34_28015) - 7628389..7628598 (+) 210 WP_070395175.1 DUF2997 domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40746.98 Da        Isoelectric Point: 8.3293

>NTDB_id=200616 BJP34_RS27990 WP_070395171.1 7623080..7624198(+) (pilT) [Moorena producens PAL-8-15-08-1]
MELMIEDLMEQMIEMGGSDMHIQAGAPVYFRVSGKLQPIDDESLNPQACQRLIFSMLNNSQRKELEQNWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDKLGLPDVVRSMSERPRGMVLVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEYVFPNIKSLIHQRQKGEDTKTFANALKGALREDPDVILVGEMRDMETIGLAISAAETGHLVFGTLHTNSAAQTVD
RIIDVFPFGQQAQIRAQLSNSLIAVFSQCLVPKKNPKPGEFGRTMAQEIMVVTPAISNLIREGKTSQVYSAIQTGVKLGM
QTLEQALAYQVKSGLISLEAGLSKSSKPDELQRIVAGSGGMNSKGGRAKATR

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=200616 BJP34_RS27990 WP_070395171.1 7623080..7624198(+) (pilT) [Moorena producens PAL-8-15-08-1]
ATGGAACTGATGATTGAAGACTTGATGGAGCAGATGATCGAGATGGGGGGGTCAGATATGCACATCCAAGCGGGTGCGCC
AGTCTACTTCCGCGTCAGTGGTAAACTCCAACCCATTGATGACGAATCCTTGAACCCTCAAGCCTGTCAGCGATTGATTT
TCAGTATGCTTAACAATTCACAGCGTAAGGAATTGGAGCAAAACTGGGAACTTGATTGTTCCTATGGTGTTAAGGGGTTG
GCTCGTTTCCGTGTCAATGTTTACAAAGAACGGGGTTGTTATGCCGCTTGCTTGAGGGCGTTGTCTTCCAAGATTCCTAA
CTTTGACAAATTGGGTTTACCGGATGTGGTGCGGTCGATGAGTGAACGACCAAGGGGAATGGTATTGGTCACCGGACCGA
CTGGTTCTGGGAAAACCACTACCCTAGCAGCCATGATTGATTTAATTAACCGCACAAGGGCAGAACATATTCTGACAGTG
GAAGACCCGATAGAATATGTGTTTCCTAACATCAAGAGCTTGATTCACCAACGTCAAAAAGGTGAAGATACTAAGACCTT
TGCCAATGCTCTGAAGGGGGCACTGCGGGAAGACCCGGATGTGATTTTGGTGGGTGAAATGCGGGATATGGAAACTATTG
GTCTAGCTATTTCTGCCGCTGAAACCGGTCACTTGGTATTTGGAACGCTACACACTAACTCAGCTGCTCAAACCGTAGAC
CGGATCATTGATGTGTTTCCATTTGGACAGCAAGCTCAAATCCGAGCTCAGCTGTCTAACTCTTTGATTGCAGTATTTAG
TCAATGTCTAGTACCGAAGAAAAATCCTAAACCCGGTGAGTTTGGTCGGACTATGGCTCAGGAAATCATGGTAGTGACAC
CTGCTATTTCTAACCTGATCCGGGAAGGAAAAACGTCTCAAGTTTACTCTGCTATCCAAACCGGTGTGAAATTGGGAATG
CAGACCCTGGAACAAGCTTTAGCTTATCAGGTGAAATCTGGCTTGATCTCTTTGGAAGCAGGACTTAGTAAAAGTTCTAA
ACCTGATGAATTGCAACGTATCGTTGCTGGTTCTGGAGGGATGAATTCTAAGGGCGGTAGAGCAAAGGCGACCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D8TYP6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

51.884

92.742

0.481
  pilT Legionella pneumophila strain ERS1305867

51.884

92.742

0.481
  pilT Pseudomonas aeruginosa PAK

50.72

93.28

0.473
  pilT Acinetobacter baumannii D1279779

50.432

93.28

0.47
  pilT Acinetobacter baumannii strain A118

50.432

93.28

0.47
  pilT Acinetobacter nosocomialis M2

50.432

93.28

0.47
  pilT Acinetobacter baylyi ADP1

49.568

93.28

0.462
  pilT Pseudomonas stutzeri DSM 10701

49.28

93.28

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.5

96.774

0.46
  pilT Neisseria gonorrhoeae MS11

48.138

93.817

0.452
  pilT Neisseria meningitidis 8013

47.851

93.817

0.449
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.094

91.667

0.441
  pilT Vibrio cholerae strain A1552

48.094

91.667

0.441
  pilU Pseudomonas stutzeri DSM 10701

38.177

94.355

0.36

Multiple sequence alignment