Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   BJI69_RS13095 Genome accession   NZ_CP017480
Coordinates   2882761..2883207 (-) Length   148 a.a.
NCBI ID   WP_046966400.1    Uniprot ID   A0A0G9HGD4
Organism   Luteibacter rhizovicinus DSM 16549 strain LJ96     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2877761..2888207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJI69_RS13085 (BJI69_13020) - 2878727..2880712 (-) 1986 WP_052767044.1 hypothetical protein -
  BJI69_RS13090 (BJI69_13025) - 2880741..2882699 (-) 1959 WP_125903049.1 tetratricopeptide repeat protein -
  BJI69_RS13095 (BJI69_13030) pilE 2882761..2883207 (-) 447 WP_046966400.1 pilin Machinery gene
  BJI69_RS13100 (BJI69_13035) - 2883508..2883948 (-) 441 WP_052767047.1 pilin -
  BJI69_RS13105 (BJI69_13040) - 2884211..2885563 (-) 1353 WP_046966401.1 glycosyltransferase family 39 protein -
  BJI69_RS13110 (BJI69_13045) - 2885709..2887796 (+) 2088 WP_046966402.1 S9 family peptidase -
  BJI69_RS13115 (BJI69_13050) - 2887964..2888143 (+) 180 WP_046966403.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15556.69 Da        Isoelectric Point: 8.9933

>NTDB_id=198654 BJI69_RS13095 WP_046966400.1 2882761..2883207(-) (pilE) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
MKNVQKGFTLIELMIVVAIIAILAAIAIPQYQNYLIRTQVSEGMNLADGNKTAVSEFYNNTGRWPSTQTSAGLPAVTSVT
GKYVTQVDASTDKGKITATFGGAEVNSKISGKKLVLSATDNGGSISWSCKTSAGTDVPDAYLPTSCRS

Nucleotide


Download         Length: 447 bp        

>NTDB_id=198654 BJI69_RS13095 WP_046966400.1 2882761..2883207(-) (pilE) [Luteibacter rhizovicinus DSM 16549 strain LJ96]
ATGAAGAACGTCCAGAAGGGCTTCACGCTCATCGAACTGATGATCGTGGTCGCCATCATCGCGATCCTGGCTGCCATCGC
CATCCCGCAGTACCAGAACTACCTCATCCGCACCCAGGTGTCGGAAGGCATGAACCTCGCTGACGGTAACAAGACCGCCG
TTTCCGAGTTCTACAACAACACGGGCCGCTGGCCGAGCACGCAGACCTCCGCTGGCCTGCCGGCGGTTACCTCTGTCACC
GGCAAGTACGTGACCCAGGTCGACGCCAGCACGGACAAGGGCAAGATCACGGCCACGTTCGGCGGCGCCGAAGTCAACAG
CAAGATCTCTGGCAAGAAGCTCGTCCTGTCGGCAACGGACAACGGCGGCTCGATCAGCTGGAGCTGCAAGACGAGCGCTG
GTACGGACGTGCCGGACGCTTACCTGCCGACCTCCTGCCGTAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G9HGD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

46.875

100

0.507
  pilE Neisseria gonorrhoeae MS11

44.72

100

0.486
  pilA Acinetobacter baumannii strain A118

45.89

98.649

0.453
  pilA2 Legionella pneumophila strain ERS1305867

44.521

98.649

0.439
  pilA2 Legionella pneumophila str. Paris

43.836

98.649

0.432
  pilA Ralstonia pseudosolanacearum GMI1000

38.415

100

0.426
  pilA Acinetobacter nosocomialis M2

42.177

99.324

0.419
  comP Acinetobacter baylyi ADP1

39.61

100

0.412
  pilA/pilA1 Eikenella corrodens VA1

38.71

100

0.405
  pilA Vibrio cholerae C6706

38.255

100

0.385
  pilA Vibrio cholerae strain A1552

38.255

100

0.385
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.255

100

0.385
  pilA Pseudomonas aeruginosa PAK

37.086

100

0.378
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.931

97.973

0.372
  pilA Vibrio campbellii strain DS40M4

34.81

100

0.372

Multiple sequence alignment