Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   XCCCN15_RS05390 Genome accession   NZ_CP017323
Coordinates   1244303..1244701 (+) Length   132 a.a.
NCBI ID   WP_040940799.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris str. CN15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1244303..1262546 1244303..1244701 within 0


Gene organization within MGE regions


Location: 1244303..1262546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XCCCN15_RS05390 pilA/pilAI 1244303..1244701 (+) 399 WP_040940799.1 pilin Machinery gene
  XCCCN15_RS05395 - 1244806..1245228 (+) 423 WP_040940800.1 pilin -
  XCCCN15_RS05400 - 1245287..1247107 (+) 1821 WP_162866362.1 hypothetical protein -
  XCCCN15_RS05405 - 1247231..1247989 (+) 759 WP_080640038.1 class I SAM-dependent methyltransferase -
  XCCCN15_RS05410 - 1248411..1249172 (+) 762 WP_162866363.1 glycosyltransferase family 9 protein -
  XCCCN15_RS23230 - 1249175..1250017 (-) 843 WP_162866364.1 glycosyltransferase -
  XCCCN15_RS05420 - 1250011..1251183 (-) 1173 WP_162866365.1 glycosyltransferase -
  XCCCN15_RS05425 - 1251168..1252031 (-) 864 WP_080640041.1 glycosyltransferase -
  XCCCN15_RS05430 - 1252081..1252932 (-) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  XCCCN15_RS05435 pilB 1253088..1254824 (+) 1737 WP_040940802.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XCCCN15_RS05440 pilR 1256209..1257603 (-) 1395 WP_040940803.1 sigma-54 dependent transcriptional regulator Regulator
  XCCCN15_RS05445 - 1257813..1259423 (-) 1611 WP_040940804.1 HAMP domain-containing sensor histidine kinase -
  XCCCN15_RS05450 sucC 1259657..1260826 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  XCCCN15_RS05455 sucD 1260851..1261726 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  XCCCN15_RS05460 - 1261830..1262240 (+) 411 WP_029217117.1 DNA-binding protein -
  XCCCN15_RS05465 - 1262244..1262546 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13892.15 Da        Isoelectric Point: 8.8371

>NTDB_id=197709 XCCCN15_RS05390 WP_040940799.1 1244303..1244701(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris str. CN15]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEINPGKTQYEVLVNEGTIPTSATQIGLTTPTARCTV
TVSSTGIQCLLKGNASKINNKTIRLNRDATAGTWTCVTGAGLEDKYKPAGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=197709 XCCCN15_RS05390 WP_040940799.1 1244303..1244701(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris str. CN15]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTAGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGATGTATCAGGACTATGTTGCCAAGTCGCAGGCGACTGCCGGTCTGGCAGAAATTAATCCTGGTAAAACGCAGTACG
AAGTATTAGTTAACGAAGGCACGATCCCTACCAGCGCTACGCAGATTGGTTTAACGACTCCCACTGCGCGCTGTACTGTA
ACTGTCAGCAGCACCGGCATCCAGTGTCTTCTCAAAGGCAACGCGTCCAAGATCAATAACAAGACTATTCGCCTTAACCG
TGATGCAACAGCTGGTACTTGGACATGTGTGACTGGCGCAGGGTTGGAAGATAAGTATAAGCCCGCCGGTTGTTCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-131)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

54.015

100

0.561
  pilA Acinetobacter baumannii strain A118

48.936

100

0.523
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.185

100

0.462
  pilA Pseudomonas aeruginosa PAK

38.312

100

0.447
  pilA/pilA1 Eikenella corrodens VA1

37.584

100

0.424
  comP Acinetobacter baylyi ADP1

37.162

100

0.417
  pilA Vibrio cholerae C6706

38.194

100

0.417
  pilA Vibrio cholerae strain A1552

38.194

100

0.417
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.194

100

0.417
  pilA2 Legionella pneumophila strain ERS1305867

39.416

100

0.409
  pilA2 Legionella pneumophila str. Paris

38.686

100

0.402
  pilA Vibrio parahaemolyticus RIMD 2210633

41.085

97.727

0.402
  pilA Haemophilus influenzae Rd KW20

41.322

91.667

0.379

Multiple sequence alignment