Detailed information NTG0197710

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: XCCCN15_RS05435
• Genome accession: NZ_CP017323
• Coordinates: 1253088..1254824 (+)
• Length: 578 a.a.
• NCBI ID: WP_040940802.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. campestris str. CN15
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1244303..1262546	1253088..1254824	within	0

Gene organization within MGE regions

Location: 1244303..1262546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XCCCN15_RS05390	pilA/pilAI	1244303..1244701 (+)	399	WP_040940799.1	pilin	Machinery gene
XCCCN15_RS05395	-	1244806..1245228 (+)	423	WP_040940800.1	pilin	-
XCCCN15_RS05400	-	1245287..1247107 (+)	1821	WP_162866362.1	hypothetical protein	-
XCCCN15_RS05405	-	1247231..1247989 (+)	759	WP_080640038.1	class I SAM-dependent methyltransferase	-
XCCCN15_RS05410	-	1248411..1249172 (+)	762	WP_162866363.1	glycosyltransferase family 9 protein	-
XCCCN15_RS23230	-	1249175..1250017 (-)	843	WP_162866364.1	glycosyltransferase	-
XCCCN15_RS05420	-	1250011..1251183 (-)	1173	WP_162866365.1	glycosyltransferase	-
XCCCN15_RS05425	-	1251168..1252031 (-)	864	WP_080640041.1	glycosyltransferase	-
XCCCN15_RS05430	-	1252081..1252932 (-)	852	WP_108133901.1	glycosyltransferase family 2 protein	-
XCCCN15_RS05435	pilB	1253088..1254824 (+)	1737	WP_040940802.1	type IV-A pilus assembly ATPase PilB	Machinery gene
XCCCN15_RS05440	pilR	1256209..1257603 (-)	1395	WP_040940803.1	sigma-54 dependent transcriptional regulator	Regulator
XCCCN15_RS05445	-	1257813..1259423 (-)	1611	WP_040940804.1	HAMP domain-containing sensor histidine kinase	-
XCCCN15_RS05450	sucC	1259657..1260826 (+)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
XCCCN15_RS05455	sucD	1260851..1261726 (+)	876	WP_011038208.1	succinate--CoA ligase subunit alpha	-
XCCCN15_RS05460	-	1261830..1262240 (+)	411	WP_029217117.1	DNA-binding protein	-
XCCCN15_RS05465	-	1262244..1262546 (+)	303	WP_011038207.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62780.99 Da
• Isoelectric Point: 5.6058

>NTDB_id=197710 XCCCN15_RS05435 WP_040940802.1 1253088..1254824(+) (pilB) [Xanthomonas campestris pv. campestris str. CN15]
MNAVISANLVGITGIARRLVQDGALDETAARTAMEQAASARVPLPQWFAERKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=197710 XCCCN15_RS05435 WP_040940802.1 1253088..1254824(+) (pilB) [Xanthomonas campestris pv. campestris str. CN15]
ATGAACGCAGTGATCTCAGCCAATCTCGTTGGCATAACCGGCATAGCTCGTCGGCTGGTTCAAGACGGCGCACTTGATGA
AACGGCGGCCCGTACCGCCATGGAGCAAGCTGCTTCAGCCAGGGTTCCTCTGCCCCAGTGGTTTGCGGAGAGAAAGTTAG
TGACTGCATCCCAGCTTGCCGCCGCAAACGCGGTTGAGTTTGGCATGCCTCTGATGGACGTTTCCGTGTTCGATGCCAGC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTACCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTCCATACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGATCAGATCCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTTGGCTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTGGACGTCTCCACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGCTTGCGGATCGATGGCCTCCTGAAGAATGTGGCCAAGGCGCCG
GTGAAGTTGAGCCAGCGCATTGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
TGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCGAGTGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTATGAGGCGGATCAGCAAAAG
CTGTTCCTGGATGCGATCCACAAGCCTTACGGCATGGTGCTGGTGACCGGGCCAACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTCAATCAGGTCCAGCAAAACGTCAAGCGCGGCATGACGTTTGCTGCGGCGTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GTTGTCGACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTGACCTTGGTCATTGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGGCGCCGCTGCCG
GAGCATGCGTTGCTGGCCGAAGGGTTTACCGCAGAGCAGATCGCTGCCGGAATCGAACTGTACGAGGCGGTCGGCTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTAC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCGGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGGGTGACGAGCCTGGCGGAGATCAATCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (50-153)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.81	98.27	0.548
pilB	Acinetobacter baylyi ADP1	55.106	98.27	0.542
pilB	Legionella pneumophila strain ERS1305867	52.021	98.443	0.512
pilB	Vibrio cholerae strain A1552	49.043	99.481	0.488
pilF	Neisseria gonorrhoeae MS11	47.887	98.27	0.471
pilB	Vibrio campbellii strain DS40M4	45.694	98.443	0.45
pilB	Vibrio parahaemolyticus RIMD 2210633	45.745	97.578	0.446
pilB/pilB1	Synechocystis sp. PCC 6803	35.61	100	0.379
pilF	Thermus thermophilus HB27	40.499	90.138	0.365