Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q5W_RS18640 Genome accession   NZ_CP017311
Coordinates   3910844..3911980 (-) Length   378 a.a.
NCBI ID   WP_009519819.1    Uniprot ID   -
Organism   Hydrogenophaga sp. PBC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3905844..3916980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5W_RS25875 - 3905972..3906508 (+) 537 WP_148663196.1 hypothetical protein -
  Q5W_RS18625 (Q5W_18625) - 3906687..3908459 (-) 1773 WP_009519816.1 hypothetical protein -
  Q5W_RS18630 (Q5W_18630) - 3909042..3909806 (+) 765 WP_138820258.1 DUF6065 family protein -
  Q5W_RS18635 (Q5W_18635) - 3909824..3910717 (-) 894 WP_009519818.1 NAD(P)-dependent oxidoreductase -
  Q5W_RS18640 (Q5W_18640) pilU 3910844..3911980 (-) 1137 WP_009519819.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q5W_RS18645 (Q5W_18645) pilT 3912006..3913049 (-) 1044 WP_009519820.1 type IV pilus twitching motility protein PilT Machinery gene
  Q5W_RS18650 (Q5W_18650) - 3913091..3913819 (+) 729 WP_035623887.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q5W_RS18655 (Q5W_18655) - 3913800..3914348 (-) 549 WP_009519822.1 DUF3617 domain-containing protein -
  Q5W_RS18660 (Q5W_18660) - 3914418..3914888 (-) 471 WP_009519823.1 Lrp/AsnC ligand binding domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41864.27 Da        Isoelectric Point: 6.7654

>NTDB_id=197576 Q5W_RS18640 WP_009519819.1 3910844..3911980(-) (pilU) [Hydrogenophaga sp. PBC]
MERDQASKFINDLLRLMLSRGGSDLFITADFPPAIKVDGAVTKVSPQPLTAGHTLALARAIMNDKQAAEFERTKECNFAI
SPPAIGRFRCNAFIQQGKVGLVLRTIPAVLPTIDKLGLPQVLKDVAMSKRGLCILVGATGSGKSTSLAAMVDWRNEHSYG
HIVTVEDPIEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIQFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPRQDNKGRFAAVEILLNSPLISDLIFKGEVAEIKEVMKKSTQMG
MQTFDQALFEAFESNLITYEDALRNADSLNDLRLQVKLNSQRAKTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=197576 Q5W_RS18640 WP_009519819.1 3910844..3911980(-) (pilU) [Hydrogenophaga sp. PBC]
ATGGAACGCGATCAGGCATCCAAGTTCATCAACGACCTGCTGCGGCTCATGCTCAGCCGGGGCGGCAGCGACCTGTTCAT
CACGGCCGACTTCCCGCCGGCCATCAAGGTGGACGGCGCCGTCACCAAGGTCTCGCCGCAGCCGCTGACGGCGGGCCACA
CCCTGGCGTTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGAGTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGCCGGCCATCGGGCGCTTCCGCTGCAACGCCTTCATCCAGCAAGGCAAGGTGGGCCTGGTGCTGCGGACCATCCC
CGCCGTGCTGCCCACGATCGACAAGCTGGGCCTGCCCCAGGTGCTGAAGGACGTGGCCATGTCCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCCACCTCGCTCGCGGCCATGGTCGATTGGCGCAACGAGCACTCTTACGGC
CATATCGTCACCGTGGAAGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGTGTGGTGACGCAGCGCGAAGTGGGCCT
GGACACCGAGAGCTGGGAGGCCGCGCTGAAGAACACCCTGCGGCAGGCACCCGACGTCATCCTCATGGGCGAAATTCGCG
ACCGCGAGACCATGGAGCACGCGATCCAGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTCTCGCAACGGCTGGTGCCGCGCCAAGACAACAAGGGCCGATTCGCCGCGGTCGAGATCCTGCTCAATT
CGCCCCTCATTTCCGACCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAAGTGATGAAGAAGAGCACCCAGATGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGAGGCCTTCGAAAGCAACCTCATCACCTACGAGGACGCGCTGCGCAACGCCGA
CTCGCTCAATGACCTGCGCCTGCAGGTCAAGCTCAACAGCCAGCGCGCGAAGACGCTGGACCTTGCCGCCGGCACCGAGC
ACTTGACGATTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.656

96.825

0.587
  pilU Acinetobacter baylyi ADP1

56.145

94.709

0.532
  pilU Vibrio cholerae strain A1552

55.172

92.063

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Acinetobacter nosocomialis M2

44.201

84.392

0.373
  pilT Acinetobacter baumannii D1279779

44.201

84.392

0.373
  pilT Acinetobacter baumannii strain A118

44.201

84.392

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Acinetobacter baylyi ADP1

42.683

86.772

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment