Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AWQ21_RS03500 Genome accession   NZ_CP016474
Coordinates   746700..747806 (+) Length   368 a.a.
NCBI ID   WP_065713348.1    Uniprot ID   A0A1B1TMC1
Organism   Picosynechococcus sp. PCC 7003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741700..752806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ21_RS03490 (AWQ21_03490) grpE 743400..744149 (-) 750 WP_065713346.1 nucleotide exchange factor GrpE -
  AWQ21_RS03495 (AWQ21_03495) pilB/pilB1 744557..746575 (+) 2019 WP_065713347.1 GspE/PulE family protein Machinery gene
  AWQ21_RS03500 (AWQ21_03500) pilT 746700..747806 (+) 1107 WP_065713348.1 type IV pilus twitching motility protein PilT Machinery gene
  AWQ21_RS03505 (AWQ21_03505) - 747873..749111 (+) 1239 WP_065713349.1 type II secretion system F family protein -
  AWQ21_RS03510 (AWQ21_03510) - 749120..749677 (+) 558 WP_065713350.1 hypothetical protein -
  AWQ21_RS03515 (AWQ21_03515) - 749674..750750 (-) 1077 WP_065713351.1 glycosyl transferase -
  AWQ21_RS03520 (AWQ21_03520) - 750779..751942 (-) 1164 WP_065713352.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40312.45 Da        Isoelectric Point: 6.7540

>NTDB_id=188502 AWQ21_RS03500 WP_065713348.1 746700..747806(+) (pilT) [Picosynechococcus sp. PCC 7003]
MDYMIEDLMEQLVEMGGSDMHIQAGAPVYFRVSGKLGPINEEPLSAQEAQKLIFSMLNNTQRKDLEQNWELDCSYGVKGL
ARFRVNVYKERGCYAACLRALSSKIPNFDQLGLPDIVREMAERPRGLVLVTGQTGSGKTTTMAAMLDLINRTRAEHILTV
EDPIEYVFPNHKSLFHQRQKGEDTKSFANALRAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTNSAASTID
RMLDVFPPIQQPQIRAMLSNSLLAVFSQCLVKKANPKPGEFGRAMAQEIMVVTPAIANLIREGKSAQVYSAIQTGMKLGM
QTMEQALAGLVATGSVTFEEALSKSGKPDELQRLVGGALGGSPTAKRR

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=188502 AWQ21_RS03500 WP_065713348.1 746700..747806(+) (pilT) [Picosynechococcus sp. PCC 7003]
ATGGATTACATGATCGAAGACCTCATGGAGCAACTCGTAGAAATGGGCGGCTCCGATATGCACATCCAGGCCGGTGCCCC
CGTTTATTTCCGAGTCAGTGGGAAACTTGGCCCCATTAACGAAGAGCCCCTTTCTGCCCAAGAGGCCCAGAAGCTCATTT
TCAGTATGCTCAACAACACCCAACGCAAGGATCTTGAGCAAAACTGGGAATTAGACTGCTCCTATGGCGTCAAAGGACTC
GCCCGCTTCCGGGTCAATGTCTACAAGGAACGGGGCTGTTATGCCGCCTGCCTGCGAGCTTTATCCTCTAAAATTCCCAA
CTTTGATCAGTTGGGTTTACCCGATATTGTGCGGGAAATGGCCGAACGTCCCCGGGGCTTGGTACTGGTGACGGGTCAAA
CGGGTTCGGGAAAAACCACCACCATGGCGGCGATGCTGGACTTGATTAACCGCACCCGGGCCGAACATATCCTCACCGTC
GAAGACCCCATCGAATACGTTTTTCCGAATCACAAAAGCCTCTTTCACCAACGGCAAAAAGGAGAAGATACCAAAAGTTT
TGCCAATGCTCTCCGGGCCGCTCTCCGGGAAGATCCAGACATTATCCTTGTGGGGGAAATGCGAGACCTCGAAACCATCT
CCTTGGCCATCTCCGCCGCGGAAACCGGCCACCTTGTCTTCGGTACTTTGCACACCAACTCCGCCGCCAGCACCATCGAC
CGGATGTTAGACGTATTCCCGCCGATCCAGCAACCCCAAATTCGGGCGATGCTCTCCAACTCCCTCCTCGCTGTTTTTAG
TCAATGTCTCGTGAAAAAAGCAAATCCGAAGCCTGGTGAATTTGGTCGGGCCATGGCTCAAGAAATTATGGTCGTCACCC
CGGCGATCGCCAACCTCATCCGTGAAGGAAAAAGCGCCCAAGTTTACTCAGCGATCCAAACGGGGATGAAACTCGGCATG
CAAACCATGGAGCAAGCCCTGGCGGGTTTAGTGGCCACAGGAAGTGTTACCTTCGAAGAAGCCTTGTCGAAGAGCGGTAA
ACCCGATGAACTCCAACGACTCGTGGGGGGGGCATTGGGGGGCAGTCCAACCGCAAAACGTCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1TMC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.623

93.75

0.503
  pilT Legionella pneumophila strain ERS1305867

53.623

93.75

0.503
  pilT Pseudomonas aeruginosa PAK

52.738

94.293

0.497
  pilT Acinetobacter baumannii D1279779

52.161

94.293

0.492
  pilT Acinetobacter baylyi ADP1

52.161

94.293

0.492
  pilT Acinetobacter baumannii strain A118

52.161

94.293

0.492
  pilT Acinetobacter nosocomialis M2

51.873

94.293

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.585

94.293

0.486
  pilT Vibrio cholerae strain A1552

50.733

92.663

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.733

92.663

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.459

97.011

0.47
  pilT Neisseria meningitidis 8013

49.852

91.576

0.457
  pilT Neisseria gonorrhoeae MS11

49.555

91.576

0.454
  pilU Pseudomonas stutzeri DSM 10701

37.892

95.38

0.361

Multiple sequence alignment