Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AWQ21_RS03495 Genome accession   NZ_CP016474
Coordinates   744557..746575 (+) Length   672 a.a.
NCBI ID   WP_065713347.1    Uniprot ID   A0A1B1TMD5
Organism   Picosynechococcus sp. PCC 7003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 739557..751575
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ21_RS03475 (AWQ21_03475) - 739818..740072 (-) 255 WP_065713344.1 sulfurtransferase TusA family protein -
  AWQ21_RS03480 (AWQ21_03480) dnaJ 740069..741205 (-) 1137 WP_065713345.1 molecular chaperone DnaJ -
  AWQ21_RS03485 (AWQ21_03485) dnaK 741327..743282 (-) 1956 WP_065715200.1 molecular chaperone DnaK -
  AWQ21_RS03490 (AWQ21_03490) grpE 743400..744149 (-) 750 WP_065713346.1 nucleotide exchange factor GrpE -
  AWQ21_RS03495 (AWQ21_03495) pilB/pilB1 744557..746575 (+) 2019 WP_065713347.1 GspE/PulE family protein Machinery gene
  AWQ21_RS03500 (AWQ21_03500) pilT 746700..747806 (+) 1107 WP_065713348.1 type IV pilus twitching motility protein PilT Machinery gene
  AWQ21_RS03505 (AWQ21_03505) - 747873..749111 (+) 1239 WP_065713349.1 type II secretion system F family protein -
  AWQ21_RS03510 (AWQ21_03510) - 749120..749677 (+) 558 WP_065713350.1 hypothetical protein -
  AWQ21_RS03515 (AWQ21_03515) - 749674..750750 (-) 1077 WP_065713351.1 glycosyl transferase -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75321.41 Da        Isoelectric Point: 5.1163

>NTDB_id=188501 AWQ21_RS03495 WP_065713347.1 744557..746575(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7003]
MTQSSLPKRTRALALRNDFSPFGNKLIQQGVVDHQQMEKALVETRRSGRPLTAIVEELTGKALSPELVRQYKKHHLFELK
ILYGLNSIDPEVQKIDIDQMGELINSLVPVELCRRHRLLPIRQIQDEVIVAMVEPDNLAAQDDLNRLFRLQNLTLRRIVI
TEDDYRQVLDGYLEQQVQIQIQQQEQATKAAEAAELKKAVDIEDLDLGVTGELDEQEDESADLMNENQASSAPVIKLVNK
VLLKALQEGASDIHIEPQENDLRIRFRKDGVLQYAFDPLPKRIVPAVTARFKIMADLDIAERRVPQDGRIRRMFKGRKVD
FRVNSLPSRYGEKIVLRILDNSSTQLGLDLLITNQDILGQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNEPGINI
STAEDPIEYTLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIA
RLDEMGVEPFMISGSLLGILAQRLMRRVCSECRIPYQPTVEELARYGISSNDRRPLTVYRANTLTPDQIQVAKEAGSLCP
KCGGGGYKGRVGVYEFMQNSEEIERLINSGATTDMIKEVAVQEGMLSILAYSLELVKEGHTTFEEVDRVTFTDSGLEAEL
KAKRKTGLVCTTCNAELQPDWLDCPYCMTPRL

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=188501 AWQ21_RS03495 WP_065713347.1 744557..746575(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7003]
ATGACCCAATCTTCCCTTCCAAAACGGACAAGAGCCCTTGCCCTCCGCAATGATTTCTCTCCTTTCGGCAACAAGCTCAT
TCAACAGGGAGTTGTAGACCATCAACAGATGGAAAAAGCCCTCGTTGAAACCCGGCGTAGTGGACGCCCCCTCACAGCCA
TTGTCGAAGAATTAACCGGGAAAGCCCTATCCCCTGAGCTAGTTCGACAATACAAAAAGCATCATCTTTTTGAACTGAAG
ATTCTCTATGGTCTCAATTCCATTGATCCAGAAGTTCAAAAGATTGACATCGATCAGATGGGAGAATTGATCAACTCCCT
AGTGCCGGTAGAATTATGCCGCCGCCATCGTCTCCTACCGATTCGTCAGATTCAAGATGAAGTCATTGTGGCGATGGTAG
AACCCGACAACCTAGCCGCCCAAGATGATTTAAATCGCCTCTTTCGTCTGCAAAATCTGACGCTACGACGCATTGTGATC
ACAGAAGACGATTATCGCCAGGTTCTAGATGGCTATTTAGAGCAACAAGTCCAGATTCAAATTCAACAGCAGGAACAGGC
AACTAAGGCGGCCGAAGCAGCAGAACTTAAAAAAGCAGTTGATATTGAAGATCTCGACCTGGGGGTGACTGGGGAATTAG
ATGAGCAGGAGGATGAAAGTGCCGACCTGATGAATGAAAATCAGGCCAGCTCTGCTCCGGTCATCAAACTCGTCAATAAA
GTTCTCCTTAAAGCCCTCCAGGAAGGGGCATCGGATATTCACATCGAACCCCAGGAAAATGATTTACGAATTCGCTTCCG
CAAAGATGGGGTGTTGCAATATGCATTTGATCCGCTGCCAAAACGTATCGTCCCCGCGGTGACCGCCCGGTTCAAAATTA
TGGCCGATCTAGACATTGCAGAACGTCGTGTACCTCAGGATGGTCGGATTCGGCGGATGTTTAAGGGGCGTAAGGTGGAC
TTTCGGGTCAATAGTTTACCCAGTCGCTATGGTGAAAAAATTGTTTTACGGATTCTCGACAATTCTTCAACTCAGTTAGG
TCTAGATTTGTTGATCACCAACCAGGACATCCTTGGGCAGGTACGGGAAATGGCCCAGCGGCCGTTTGGTCTGATTTTGG
TGACGGGGCCCACGGGGTCAGGAAAATCAACGACGTTGTATTCGATCCTGGCGGAACGCAATGAGCCGGGCATCAACATC
AGTACGGCAGAAGACCCCATTGAATATACGTTGCCGGGTATTACGCAGGTGCAGGTCATCCGGGAAAAGGGGATGGATTT
TGCGTCAATTTTGCGGGCATTTTTGCGGCAGGATCCGGATGTCATTCTTGTGGGGGAAACCCGGGATAAGGAAACGGCAA
AAACAGCCATTGAAGCGGCTTTAACGGGGCACTTGGTTTTAACCACTCTCCACACTAACGATGCACCAGGGGCGATCGCC
CGCCTAGATGAGATGGGAGTTGAACCCTTTATGATTTCCGGGTCACTGTTGGGGATCCTAGCCCAGCGGTTAATGCGACG
GGTTTGTAGCGAATGCCGTATCCCCTATCAGCCCACAGTGGAAGAGTTGGCACGCTACGGCATTTCCAGTAATGATCGCC
GTCCTTTAACGGTGTACCGGGCCAATACCTTGACACCAGATCAGATTCAAGTAGCCAAAGAAGCAGGCAGTCTCTGTCCC
AAATGTGGTGGCGGCGGCTATAAAGGCCGGGTCGGGGTCTATGAGTTCATGCAAAATAGCGAGGAAATTGAACGACTGAT
CAACAGCGGCGCGACCACAGACATGATTAAAGAAGTGGCGGTGCAAGAGGGCATGTTGAGTATCTTGGCCTACAGTTTGG
AATTGGTCAAAGAAGGCCATACTACTTTTGAAGAGGTAGATCGCGTCACCTTTACTGATTCTGGTCTTGAGGCAGAACTC
AAAGCGAAACGAAAAACGGGCCTTGTTTGTACCACTTGTAATGCTGAATTGCAGCCGGATTGGCTAGATTGCCCCTACTG
TATGACGCCCCGCCTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-176)

Type II/IV secretion system protein (T2SSE)

(240-507)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1TMD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.037

100

0.653
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.854

93.452

0.363

Multiple sequence alignment