Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHL_RS03220 Genome accession   NZ_CP015576
Coordinates   577864..578931 (+) Length   355 a.a.
NCBI ID   WP_063997948.1    Uniprot ID   A0A562XC04
Organism   Campylobacter hyointestinalis subsp. lawsonii CCUG 27631     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 572864..583931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHL_RS03185 (CHL_0643) - 572947..574047 (-) 1101 WP_063997941.1 metallophosphoesterase -
  CHL_RS03190 (CHL_0644) hisG 574228..574866 (-) 639 WP_063997942.1 ATP phosphoribosyltransferase -
  CHL_RS03195 (CHL_0645) - 574854..575486 (-) 633 WP_063997943.1 type III pantothenate kinase -
  CHL_RS03200 (CHL_0646) - 575473..575799 (-) 327 WP_063997944.1 hypothetical protein -
  CHL_RS03205 (CHL_0647) - 575801..576799 (-) 999 WP_063997945.1 hypothetical protein -
  CHL_RS03210 (CHL_0648) - 576804..577364 (-) 561 WP_063997946.1 hypothetical protein -
  CHL_RS03215 (CHL_0649) gatC 577572..577859 (+) 288 WP_063997947.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CHL_RS03220 (CHL_0650) pilT 577864..578931 (+) 1068 WP_063997948.1 type IV pilus twitching motility protein PilT Machinery gene
  CHL_RS03225 (CHL_0651) - 578934..579500 (+) 567 WP_063997949.1 CvpA family protein -
  CHL_RS03230 (CHL_0652) - 579503..579985 (+) 483 WP_063998972.1 Fur family transcriptional regulator -
  CHL_RS03235 (CHL_0653) lysS 579989..581494 (+) 1506 WP_235598091.1 lysine--tRNA ligase -
  CHL_RS03240 (CHL_0654) - 581496..582740 (+) 1245 WP_063997951.1 serine hydroxymethyltransferase -
  CHL_RS03245 (CHL_0655) - 582740..583282 (+) 543 WP_063997952.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39428.25 Da        Isoelectric Point: 6.8314

>NTDB_id=181100 CHL_RS03220 WP_063997948.1 577864..578931(+) (pilT) [Campylobacter hyointestinalis subsp. lawsonii CCUG 27631]
MSINIEKLLKTLAYNKGSDLHLVARSEPQIRISGVLRPLQCGILDGDEVENLCYTVLTDSQKSKLEENKELDFSIELPGI
GRFRGNCYYTMNGSLAAAFRMIPLEIPSLDDLKAPEIFKDIVKREKGLVLVTGPTGSGKSTTLAAMLNEINLTERKHIIT
IEDPIEFVHKNKKSLFSHRNIGTDTKTFASALKYALREDPDIILVGDLRDIEAISIAISAAETGHLVFGTLHTNSAIQTI
NRIVDSFDESEQSQIRNMLSISLHAVISQALVPLTSGGRIAIHEILINNPAISNLIRENKIHQIYSQLQLNQQNTGMQTQ
TQDMERAFKSGLITKDNALRYSTNKQELIGKIGHL

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=181100 CHL_RS03220 WP_063997948.1 577864..578931(+) (pilT) [Campylobacter hyointestinalis subsp. lawsonii CCUG 27631]
ATGAGTATAAATATAGAAAAGCTTCTAAAAACCTTGGCTTATAACAAAGGTAGCGATTTACATCTAGTCGCTAGAAGCGA
ACCTCAGATCCGTATAAGTGGAGTTTTGAGACCTTTGCAGTGCGGAATTTTAGATGGAGATGAAGTAGAAAATTTATGTT
ATACGGTGCTTACGGACTCACAAAAAAGCAAACTAGAAGAAAATAAAGAATTAGACTTTTCTATAGAGTTGCCAGGCATA
GGTCGTTTTAGGGGAAATTGCTATTATACTATGAATGGTAGTTTGGCTGCGGCTTTTCGTATGATTCCTTTAGAAATCCC
AAGTTTAGATGATCTAAAAGCTCCTGAGATATTTAAGGATATAGTAAAACGAGAAAAAGGGCTTGTTTTGGTCACAGGAC
CTACTGGTAGTGGCAAATCAACTACTTTAGCAGCTATGTTAAATGAGATAAACTTAACTGAGAGAAAGCATATCATTACT
ATAGAAGATCCTATAGAATTTGTTCATAAAAATAAAAAATCTCTCTTTTCTCATAGAAATATAGGAACCGATACAAAGAC
ATTTGCTAGTGCCTTAAAGTATGCTCTTAGGGAAGATCCAGATATTATATTAGTAGGCGATCTGCGTGATATAGAAGCTA
TATCTATAGCCATATCTGCAGCAGAAACAGGGCATTTGGTATTTGGTACTTTACATACAAACTCAGCCATACAGACTATA
AATAGAATAGTAGATAGTTTTGATGAGAGTGAGCAATCTCAGATAAGAAATATGCTTAGTATATCTTTACATGCTGTCAT
ATCTCAAGCACTAGTTCCATTAACTAGTGGTGGTAGGATAGCCATACATGAGATACTTATCAACAATCCTGCTATATCAA
ATTTAATAAGAGAGAATAAAATACATCAAATTTATTCTCAGTTGCAGTTAAATCAGCAAAATACCGGTATGCAAACGCAA
ACTCAAGATATGGAAAGAGCATTTAAATCAGGGCTTATAACAAAAGATAATGCCCTTAGATACTCGACAAATAAACAAGA
ATTAATAGGAAAGATAGGGCATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A562XC04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.738

97.746

0.515
  pilT Neisseria meningitidis 8013

52.45

97.746

0.513
  pilT Vibrio cholerae strain A1552

52.568

93.239

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.568

93.239

0.49
  pilT Legionella pneumophila strain Lp02

50.437

96.62

0.487
  pilT Legionella pneumophila strain ERS1305867

50.437

96.62

0.487
  pilT Pseudomonas stutzeri DSM 10701

50.893

94.648

0.482
  pilT Pseudomonas aeruginosa PAK

50.298

94.648

0.476
  pilT Acinetobacter nosocomialis M2

50.298

94.648

0.476
  pilT Acinetobacter baumannii D1279779

50.298

94.648

0.476
  pilT Acinetobacter baumannii strain A118

50.298

94.648

0.476
  pilT Acinetobacter baylyi ADP1

51.057

93.239

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

98.028

0.434
  pilU Acinetobacter baylyi ADP1

38.506

98.028

0.377
  pilU Pseudomonas stutzeri DSM 10701

37.853

99.718

0.377

Multiple sequence alignment