Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   AYO71_RS25990 Genome accession   NZ_CP014947
Coordinates   5791055..5791477 (-) Length   140 a.a.
NCBI ID   WP_041477820.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain D26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5786055..5796477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYO71_RS25975 (AYO71_25975) - 5786923..5789874 (+) 2952 WP_041477822.1 DUF748 domain-containing protein -
  AYO71_RS25980 (AYO71_25980) - 5789871..5790170 (+) 300 WP_041477821.1 DUF2845 domain-containing protein -
  AYO71_RS25985 (AYO71_25985) - 5790406..5790759 (-) 354 WP_016773155.1 BON domain-containing protein -
  AYO71_RS25990 (AYO71_25990) pilA/pilAI 5791055..5791477 (-) 423 WP_041477820.1 pilin Machinery gene
  AYO71_RS25995 (AYO71_25995) pilB 5791704..5793404 (+) 1701 WP_041477819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AYO71_RS26000 (AYO71_26000) pilC 5793407..5794624 (+) 1218 WP_041477818.1 type II secretion system F family protein Machinery gene
  AYO71_RS26005 (AYO71_26005) pilD 5794626..5795498 (+) 873 WP_041477817.1 prepilin peptidase Machinery gene
  AYO71_RS26010 (AYO71_26010) coaE 5795495..5796118 (+) 624 WP_041477816.1 dephospho-CoA kinase -
  AYO71_RS26015 (AYO71_26015) yacG 5796115..5796315 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14144.32 Da        Isoelectric Point: 8.4918

>NTDB_id=175299 AYO71_RS25990 WP_041477820.1 5791055..5791477(-) (pilA/pilAI) [Pseudomonas koreensis strain D26]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPSLAAVKGDNVTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVLGKTITLTRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=175299 AYO71_RS25990 WP_041477820.1 5791055..5791477(-) (pilA/pilAI) [Pseudomonas koreensis strain D26]
ATGAAAAAACAACAAGGTTTCACATTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACGATCGCTTT
GCCGCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAAGCTTGGCGGCGGTGAAAGGCGATAACGTCACCACTACCTCCAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCAGACGGAGCTGGAACGATTGTATGTACGTTGGCCAACGCGCCAGCGCCTGTATTAGG
CAAAACCATCACGCTGACTCGTACAGCTGCGGGTGGTTGGAGCTGTGGCACTACGGTAGCTCCTGACTATTCACCGAAAG
GCTGTAACGCTACTGGTACTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.365

97.857

0.493
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.29

100

0.457
  pilA Vibrio cholerae C6706

41.29

100

0.457
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.29

100

0.457
  comP Acinetobacter baylyi ADP1

41.447

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.443
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila str. Paris

39.041

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

42.222

96.429

0.407
  pilA2 Legionella pneumophila strain ERS1305867

36.986

100

0.386
  pilA/pilA1 Eikenella corrodens VA1

39.259

96.429

0.379

Multiple sequence alignment