Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AYO71_RS25995 Genome accession   NZ_CP014947
Coordinates   5791704..5793404 (+) Length   566 a.a.
NCBI ID   WP_041477819.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain D26     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5786704..5798404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYO71_RS25975 (AYO71_25975) - 5786923..5789874 (+) 2952 WP_041477822.1 DUF748 domain-containing protein -
  AYO71_RS25980 (AYO71_25980) - 5789871..5790170 (+) 300 WP_041477821.1 DUF2845 domain-containing protein -
  AYO71_RS25985 (AYO71_25985) - 5790406..5790759 (-) 354 WP_016773155.1 BON domain-containing protein -
  AYO71_RS25990 (AYO71_25990) pilA/pilAI 5791055..5791477 (-) 423 WP_041477820.1 pilin Machinery gene
  AYO71_RS25995 (AYO71_25995) pilB 5791704..5793404 (+) 1701 WP_041477819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AYO71_RS26000 (AYO71_26000) pilC 5793407..5794624 (+) 1218 WP_041477818.1 type II secretion system F family protein Machinery gene
  AYO71_RS26005 (AYO71_26005) pilD 5794626..5795498 (+) 873 WP_041477817.1 prepilin peptidase Machinery gene
  AYO71_RS26010 (AYO71_26010) coaE 5795495..5796118 (+) 624 WP_041477816.1 dephospho-CoA kinase -
  AYO71_RS26015 (AYO71_26015) yacG 5796115..5796315 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  AYO71_RS26020 (AYO71_26020) - 5796323..5796538 (-) 216 WP_041477815.1 hypothetical protein -
  AYO71_RS26025 (AYO71_26025) - 5796604..5797293 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  AYO71_RS26030 (AYO71_26030) - 5797484..5798110 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62633.75 Da        Isoelectric Point: 6.1319

>NTDB_id=175300 AYO71_RS25995 WP_041477819.1 5791704..5793404(+) (pilB) [Pseudomonas koreensis strain D26]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLHCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKIYRVRMRTDGMLREVAKPPIQLAGRIAARL
KIMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=175300 AYO71_RS25995 WP_041477819.1 5791704..5793404(+) (pilB) [Pseudomonas koreensis strain D26]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAAATAGCTTCCGAACATTTCGGCATGGCGCTGCTCGATCTGCATTGCCTCGACAAAGAGACTCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTTCGTCAGCACTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCAAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AACTCAGCGACGCCATCGACAAGTTCTTCGACAGCCACGCCTCGGGACTGGAAGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCTGACGACGCCCCGGTCGTACGTTT
TGTGCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCGTCCGACCTGCATTTCGAACCCTACGAGAAAATCTATCGGG
TGCGCATGCGTACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCGCGCTTG
AAGATAATGGCCAGTCTGGATATCTCGGAACGGCGCAAACCTCAGGACGGCCGCATCAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTCAACACCTTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCTTGGGTTACGAGGCCGAACAAAAGGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACCGGTTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGATCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTCGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCAATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAATAGCGCAGCGGA
AACCCTGACACGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGTTGTGCGCACACTGCAAGCGGCCGCTCGAAATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTTACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGGTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGATTGATCATGGCCGAGGGCAATTCGCTGGAAATCGATATTCAGA
TGCGCCGCGACGGCTTCGCCGATCTGCGCACTTCCGGGCTGCACAAGGCGATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.522

99.823

0.574
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.624

99.117

0.502
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilF Neisseria gonorrhoeae MS11

49.645

99.647

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.024

94.17

0.367

Multiple sequence alignment