Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AYO71_RS26000 Genome accession   NZ_CP014947
Coordinates   5793407..5794624 (+) Length   405 a.a.
NCBI ID   WP_041477818.1    Uniprot ID   -
Organism   Pseudomonas koreensis strain D26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5788407..5799624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AYO71_RS25980 (AYO71_25980) - 5789871..5790170 (+) 300 WP_041477821.1 DUF2845 domain-containing protein -
  AYO71_RS25985 (AYO71_25985) - 5790406..5790759 (-) 354 WP_016773155.1 BON domain-containing protein -
  AYO71_RS25990 (AYO71_25990) pilA/pilAI 5791055..5791477 (-) 423 WP_041477820.1 pilin Machinery gene
  AYO71_RS25995 (AYO71_25995) pilB 5791704..5793404 (+) 1701 WP_041477819.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AYO71_RS26000 (AYO71_26000) pilC 5793407..5794624 (+) 1218 WP_041477818.1 type II secretion system F family protein Machinery gene
  AYO71_RS26005 (AYO71_26005) pilD 5794626..5795498 (+) 873 WP_041477817.1 prepilin peptidase Machinery gene
  AYO71_RS26010 (AYO71_26010) coaE 5795495..5796118 (+) 624 WP_041477816.1 dephospho-CoA kinase -
  AYO71_RS26015 (AYO71_26015) yacG 5796115..5796315 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  AYO71_RS26020 (AYO71_26020) - 5796323..5796538 (-) 216 WP_041477815.1 hypothetical protein -
  AYO71_RS26025 (AYO71_26025) - 5796604..5797293 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  AYO71_RS26030 (AYO71_26030) - 5797484..5798110 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  AYO71_RS26035 (AYO71_26035) - 5798107..5798634 (+) 528 WP_041477813.1 MOSC domain-containing protein -
  AYO71_RS26040 (AYO71_26040) - 5798686..5798859 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44043.72 Da        Isoelectric Point: 10.1214

>NTDB_id=175301 AYO71_RS26000 WP_041477818.1 5793407..5794624(+) (pilC) [Pseudomonas koreensis strain D26]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=175301 AYO71_RS26000 WP_041477818.1 5793407..5794624(+) (pilC) [Pseudomonas koreensis strain D26]
ATGGCGGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACGGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGTAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCCACGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGTGTGGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCACGTTTTGCCCGCACCCTGTCGACCACATTCGCAGCCGGTGTGCCGCTGGTGGAAGCACTGG
ATTCGGTCGCCGGTGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAACTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCCAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.599

98.519

0.39

Multiple sequence alignment