Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GS3922_RS03050 Genome accession   NZ_CP014342
Coordinates   645793..646848 (+) Length   351 a.a.
NCBI ID   WP_063165122.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain KCTC3922(T)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640793..651848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GS3922_RS03035 (GS3922_03035) - 641965..642744 (+) 780 WP_063165119.1 PRC-barrel domain-containing protein -
  GS3922_RS03040 (GS3922_03040) - 642761..644119 (+) 1359 WP_063165120.1 G5 domain-containing protein -
  GS3922_RS03045 (GS3922_03045) - 644116..645780 (+) 1665 WP_063165121.1 GspE/PulE family protein -
  GS3922_RS03050 (GS3922_03050) pilT 645793..646848 (+) 1056 WP_063165122.1 type IV pilus twitching motility protein PilT Machinery gene
  GS3922_RS03055 (GS3922_03055) - 646835..648046 (+) 1212 WP_063165123.1 type II secretion system F family protein -
  GS3922_RS03060 (GS3922_03060) - 648134..648676 (+) 543 WP_063165124.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GS3922_RS03065 (GS3922_03065) - 648705..649451 (+) 747 WP_063165125.1 prepilin peptidase -
  GS3922_RS03070 (GS3922_03070) pilM 649478..650407 (+) 930 WP_063165126.1 type IV pilus biogenesis protein PilM -
  GS3922_RS03075 (GS3922_03075) - 650397..650972 (+) 576 WP_063167292.1 PilN domain-containing protein -
  GS3922_RS03080 (GS3922_03080) - 650969..651637 (+) 669 WP_063165127.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38536.77 Da        Isoelectric Point: 8.0025

>NTDB_id=170500 GS3922_RS03050 WP_063165122.1 645793..646848(+) (pilT) [Geobacillus subterraneus strain KCTC3922(T)]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRLNGELKAYGHERLRLEETEQMAKAIIPPPLWQQFQEAGELDFSYGIAGV
SRFRFNVFKQRSCVSLAVRLIPMRIPTLEELGMPSVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDVILVGEMRDLETIQTAVTAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNGRKSGRTAALEILINNAAVANLIRNKKEHQIVNIMQTSRNAGMQTME
MSIKELVADGRIDPLAAEVYLAGERGEHGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=170500 GS3922_RS03050 WP_063165122.1 645793..646848(+) (pilT) [Geobacillus subterraneus strain KCTC3922(T)]
ATGAAGGAAAAGCTTGAGGCGTTATTGCGCGCCGCCTTTGAATGCCGCGCGTCCGATGTGCATTTAACCGTCGGCGCGCC
GCCGATTTTCCGCTTGAATGGGGAGCTGAAGGCATACGGCCATGAGCGGCTTCGTTTGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCGCCGCCGCTTTGGCAGCAGTTCCAAGAAGCGGGGGAGCTCGATTTTTCGTACGGCATAGCCGGCGTC
TCGCGCTTCCGTTTCAATGTGTTTAAACAGCGGTCGTGCGTGTCGCTCGCTGTGCGCCTCATTCCGATGCGCATTCCGAC
GCTTGAGGAGCTCGGCATGCCGAGCGTCCTGAAACGCATCGCTGAAAAGCCGCACGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCGATGATCGACTATATGAACAAAACGATGTCGAAGCATATCATTACGCTC
GAGGATCCGATCGAATATTTGCATAAACATGGAAAATCGATCATCGACCAGCGTGAGATCGGATTTGACACCGGCAGTTT
TGCTGCTGGTTTGCGCGCTGCGCTCCGCCAAGATCCGGATGTCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGGTCACCGCCGCGGAAACGGGGCATTTAGTGTTAGGCACGCTTCATACAGCGAGCGCGCCGGCGGCGATCGAG
CGGATGATTGACGTCTTTCCGCCGGCCCAGCAGGCACAGATTCGTCTCCAGCTCGCGTCCGTGCTTGTGGCCATCATCGC
CCAGCGTCTGTTTCCAAACGGCCGCAAAAGCGGGCGGACAGCCGCGCTTGAGATTTTAATCAACAATGCGGCCGTTGCCA
ATTTGATCCGCAACAAAAAGGAGCATCAAATCGTCAACATTATGCAGACGAGCCGCAACGCGGGCATGCAGACGATGGAA
ATGAGCATCAAAGAGCTTGTGGCCGACGGACGCATCGATCCGCTGGCGGCTGAAGTGTATTTGGCAGGGGAGCGTGGCGA
GCATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.292

90.883

0.484
  pilT Neisseria meningitidis 8013

47.605

95.157

0.453
  pilT Neisseria gonorrhoeae MS11

47.605

95.157

0.453
  pilT Acinetobacter baumannii strain A118

47.734

94.302

0.45
  pilT Acinetobacter baumannii D1279779

47.734

94.302

0.45
  pilT Vibrio cholerae strain A1552

48.466

92.877

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.466

92.877

0.45
  pilT Pseudomonas aeruginosa PAK

48.916

92.023

0.45
  pilT Acinetobacter nosocomialis M2

47.432

94.302

0.447
  pilT Acinetobacter baylyi ADP1

47.546

92.877

0.442
  pilT Pseudomonas stutzeri DSM 10701

47.368

92.023

0.436
  pilT Legionella pneumophila strain ERS1305867

47.188

91.168

0.43
  pilT Legionella pneumophila strain Lp02

47.188

91.168

0.43
  pilU Pseudomonas stutzeri DSM 10701

42.553

93.732

0.399
  pilU Vibrio cholerae strain A1552

41.337

93.732

0.387

Multiple sequence alignment