Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AWU82_RS26135 Genome accession   NZ_CP014205
Coordinates   5732351..5732788 (-) Length   145 a.a.
NCBI ID   WP_064382076.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5727351..5737788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS26120 (AWU82_16185) - 5728282..5731233 (+) 2952 WP_064382074.1 DUF748 domain-containing protein -
  AWU82_RS26125 (AWU82_16190) - 5731233..5731529 (+) 297 WP_064382075.1 DUF2845 domain-containing protein -
  AWU82_RS26130 (AWU82_16195) - 5731696..5732052 (-) 357 WP_011335977.1 BON domain-containing protein -
  AWU82_RS26135 (AWU82_16200) pilA 5732351..5732788 (-) 438 WP_064382076.1 pilin Machinery gene
  AWU82_RS26140 (AWU82_16205) pilB 5733015..5734715 (+) 1701 WP_064382077.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AWU82_RS26145 (AWU82_16210) pilC 5734718..5735935 (+) 1218 WP_064382078.1 type II secretion system F family protein Machinery gene
  AWU82_RS26150 (AWU82_16215) pilD 5735937..5736809 (+) 873 WP_064382079.1 prepilin peptidase Machinery gene
  AWU82_RS26155 (AWU82_16220) coaE 5736806..5737429 (+) 624 WP_064382080.1 dephospho-CoA kinase -
  AWU82_RS26160 (AWU82_16225) yacG 5737426..5737626 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14519.57 Da        Isoelectric Point: 8.1096

>NTDB_id=169390 AWU82_RS26135 WP_064382076.1 5732351..5732788(-) (pilA) [Pseudomonas glycinae strain MS586]
MNKQQGFTLIELLIVVAIIGILATVALPQYSKYQARSKVTAGLAEISAMKVPFEDTINQGTAPDITNVNGGTAATSNCTL
TVTGTAATGAGTITCTLVNAPGPVLGKTITLTRSGAATGATSGVWTCASTVNNEYAPRGCPGSGA

Nucleotide


Download         Length: 438 bp        

>NTDB_id=169390 AWU82_RS26135 WP_064382076.1 5732351..5732788(-) (pilA) [Pseudomonas glycinae strain MS586]
ATGAACAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATCGTCGTTGCGATCATCGGTATTTTGGCCACTGTGGCGCT
GCCCCAGTATTCCAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGTTGGCCGAGATCAGTGCGATGAAGGTGCCGTTCG
AGGACACCATCAACCAAGGTACCGCTCCGGATATAACCAATGTGAATGGCGGTACAGCCGCCACCTCCAACTGTACGTTG
ACGGTTACAGGCACCGCGGCGACTGGCGCTGGCACCATTACATGTACGCTGGTTAATGCACCAGGTCCGGTGCTGGGTAA
AACCATTACGCTCACCCGCTCTGGCGCGGCCACCGGTGCCACGTCGGGTGTCTGGACATGTGCTTCTACGGTCAACAATG
AATACGCGCCGAGAGGTTGCCCTGGCAGCGGCGCTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

49.65

98.621

0.49
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.183

97.931

0.462
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.384

100

0.441
  pilA Vibrio cholerae strain A1552

42.384

100

0.441
  pilA Vibrio cholerae C6706

42.384

100

0.441
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.972

97.241

0.428
  pilA Pseudomonas aeruginosa PAK

40.523

100

0.428
  comP Acinetobacter baylyi ADP1

39.474

100

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.141

97.931

0.393
  pilA2 Legionella pneumophila str. Paris

40.141

97.931

0.393
  pilA Vibrio parahaemolyticus RIMD 2210633

42.308

89.655

0.379
  pilA Ralstonia pseudosolanacearum GMI1000

39.855

95.172

0.379

Multiple sequence alignment