Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AWU82_RS26140 Genome accession   NZ_CP014205
Coordinates   5733015..5734715 (+) Length   566 a.a.
NCBI ID   WP_064382077.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5728015..5739715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS26120 (AWU82_16185) - 5728282..5731233 (+) 2952 WP_064382074.1 DUF748 domain-containing protein -
  AWU82_RS26125 (AWU82_16190) - 5731233..5731529 (+) 297 WP_064382075.1 DUF2845 domain-containing protein -
  AWU82_RS26130 (AWU82_16195) - 5731696..5732052 (-) 357 WP_011335977.1 BON domain-containing protein -
  AWU82_RS26135 (AWU82_16200) pilA 5732351..5732788 (-) 438 WP_064382076.1 pilin Machinery gene
  AWU82_RS26140 (AWU82_16205) pilB 5733015..5734715 (+) 1701 WP_064382077.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AWU82_RS26145 (AWU82_16210) pilC 5734718..5735935 (+) 1218 WP_064382078.1 type II secretion system F family protein Machinery gene
  AWU82_RS26150 (AWU82_16215) pilD 5735937..5736809 (+) 873 WP_064382079.1 prepilin peptidase Machinery gene
  AWU82_RS26155 (AWU82_16220) coaE 5736806..5737429 (+) 624 WP_064382080.1 dephospho-CoA kinase -
  AWU82_RS26160 (AWU82_16225) yacG 5737426..5737626 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  AWU82_RS26165 (AWU82_16230) - 5737633..5737848 (-) 216 WP_064382081.1 hypothetical protein -
  AWU82_RS26170 (AWU82_16235) - 5737914..5738603 (-) 690 WP_064382082.1 energy-coupling factor ABC transporter permease -
  AWU82_RS26175 (AWU82_16240) - 5738600..5739067 (-) 468 WP_064382083.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62824.86 Da        Isoelectric Point: 5.9787

>NTDB_id=169391 AWU82_RS26140 WP_064382077.1 5733015..5734715(+) (pilB) [Pseudomonas glycinae strain MS586]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVDDSKQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGSFTIYEPAGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTRMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=169391 AWU82_RS26140 WP_064382077.1 5733015..5734715(+) (pilB) [Pseudomonas glycinae strain MS586]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAGGGAAACTCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATCCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAAAAATTCTTCGACACCCACGCCACCGGCCTGGAGGAAATGGCCGATGTCGACCTCGACGGG
CTGGACATCGAGTCGGTCGACGACAGCAAACAGGACACGCTCGGCGGCATCGATGCCGACGATGCCCCGGTGGTGCGTTT
TGTTCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAAAAAAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAGGTGGCGAAGCCGCCGATTCAGCTCGCCGGGCGTATTGCCGCGCGGCTG
AAGGTCATGGCCAGCCTCGACATTTCAGAACGACGCAAACCTCAGGACGGGCGGATCAAGATGCGTCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGCGAAAAAGTGGTGATTCGAATCCTCGACCCCTCCAGCG
CCCAGATCGGCATTGATGCCCTCGGCTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAACAGCCCCAGGGC
ATGATTCTGGTGACAGGTCCCACCGGCTCGGGCAAGACCGTCTCGCTGTACACCGGGCTGAATATCCTCAATACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAAG
GGATGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTAGTATTGTCCACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACTCGCCTGCACAACATGGGCATTCCCGGCTTCAACATTGCGACCTCGGTGAGCCTGATCATTGCCCAGCGTC
TTGCGCGAAAACTCTGCAGCCATTGCAAGAAACCCATCGAGATCCCCCGCGAGACCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCAGTTTCACGATCTATGAGCCCGCCGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACCCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATACCCGGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACATCGGGCCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.025

100

0.581
  pilB Acinetobacter baumannii D1279779

57.496

100

0.576
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

49.383

100

0.495
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362

Multiple sequence alignment