Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AWU82_RS26145 Genome accession   NZ_CP014205
Coordinates   5734718..5735935 (+) Length   405 a.a.
NCBI ID   WP_064382078.1    Uniprot ID   -
Organism   Pseudomonas glycinae strain MS586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5729718..5740935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWU82_RS26125 (AWU82_16190) - 5731233..5731529 (+) 297 WP_064382075.1 DUF2845 domain-containing protein -
  AWU82_RS26130 (AWU82_16195) - 5731696..5732052 (-) 357 WP_011335977.1 BON domain-containing protein -
  AWU82_RS26135 (AWU82_16200) pilA 5732351..5732788 (-) 438 WP_064382076.1 pilin Machinery gene
  AWU82_RS26140 (AWU82_16205) pilB 5733015..5734715 (+) 1701 WP_064382077.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AWU82_RS26145 (AWU82_16210) pilC 5734718..5735935 (+) 1218 WP_064382078.1 type II secretion system F family protein Machinery gene
  AWU82_RS26150 (AWU82_16215) pilD 5735937..5736809 (+) 873 WP_064382079.1 prepilin peptidase Machinery gene
  AWU82_RS26155 (AWU82_16220) coaE 5736806..5737429 (+) 624 WP_064382080.1 dephospho-CoA kinase -
  AWU82_RS26160 (AWU82_16225) yacG 5737426..5737626 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  AWU82_RS26165 (AWU82_16230) - 5737633..5737848 (-) 216 WP_064382081.1 hypothetical protein -
  AWU82_RS26170 (AWU82_16235) - 5737914..5738603 (-) 690 WP_064382082.1 energy-coupling factor ABC transporter permease -
  AWU82_RS26175 (AWU82_16240) - 5738600..5739067 (-) 468 WP_064382083.1 hypothetical protein -
  AWU82_RS26180 (AWU82_16245) - 5739198..5739824 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -
  AWU82_RS26185 (AWU82_16250) - 5739821..5740348 (+) 528 WP_064382084.1 MOSC domain-containing protein -
  AWU82_RS26190 (AWU82_16255) - 5740400..5740573 (+) 174 WP_003228304.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44058.69 Da        Isoelectric Point: 10.0476

>NTDB_id=169392 AWU82_RS26145 WP_064382078.1 5734718..5735935(+) (pilC) [Pseudomonas glycinae strain MS586]
MAVKAAKISVYAWEGTDRKGSKVTGELSGQNPALVKAQLRKQGINPGKVRKKSASLLSFGQRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQAVFSGFGAELPAFTLMVISLSEFMQQWWWAILGLLVAGFFGTR
HALKKSQALRDRRDAWFLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGIFPNMAVQMTAIGEESGALDDMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=169392 AWU82_RS26145 WP_064382078.1 5734718..5735935(+) (pilC) [Pseudomonas glycinae strain MS586]
ATGGCGGTCAAGGCAGCAAAAATCAGCGTCTACGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTGACCGGCGAGTT
GAGCGGCCAGAACCCCGCGCTGGTCAAGGCCCAATTGCGCAAGCAGGGCATCAACCCCGGCAAGGTACGCAAGAAGTCCG
CTTCGCTACTGAGCTTCGGCCAGCGCATCAAGGCCCAGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGTGTGCCACTGTTGCAGTCGTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTGAAACAGGAGGTCGCCGCCGGTAACAGTTTCGCCGCCGCGCTGCGCAAGAAGCCGCAATATTTCGACGAGTTGTACT
GCAACCTGGTGGATGCCGGCGAGCAGTCCGGCGCCCTCGACACCCTGCTGGAGCGGGTTGCGACCTACAAGGAAAAGAGT
GAAAGCCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCGACCGCCGTCGTGCTGGTGGCGGCGGTGGTCACCGGGAT
TCTGCTGGTCAAGGTAGTGCCGCAGTTCCAGGCGGTATTCTCCGGCTTCGGTGCCGAACTGCCGGCCTTCACCTTGATGG
TCATCAGCCTGTCCGAATTCATGCAGCAATGGTGGTGGGCAATTCTCGGTTTGCTGGTGGCCGGTTTTTTCGGTACCCGC
CATGCCCTGAAAAAATCCCAGGCCCTGCGCGACCGGCGCGACGCCTGGTTTCTCAAGCTGCCGCTGGTGGGCACATTGAT
GTACAAGTCCGCCGTGGCAAGGTTTGCCCGGACCTTATCCACCACCTTCGCAGCCGGCGTGCCGCTGGTGGAAGCGCTGG
ATTCGGTGGCCGGGGCCACCGGCAACGTGGTGTTCAAGCGCGCCGTGCTGCGTATCCGCCAGGACGTTTCCACCGGCATG
CAGCTGAATTTCTCGATGCGCTCCACCGGCATCTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAAGAGTCCGG
CGCGCTGGACGATATGCTCGACAAGGTCGCGGGGTTCTATGAGGACGAGGTGGACAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTCGGGGTGATCGTCGGCGGTCTGGTGGTGGCGATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.572

100

0.753
  pilC Acinetobacter baumannii D1279779

58.824

100

0.593
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilG Neisseria meningitidis 44/76-A

42.963

100

0.43
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilC Vibrio cholerae strain A1552

43.939

97.778

0.43
  pilC Vibrio campbellii strain DS40M4

41.463

100

0.42
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment