Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AT984_RS15250 Genome accession   NZ_CP013692
Coordinates   3519258..3520394 (-) Length   378 a.a.
NCBI ID   WP_058720826.1    Uniprot ID   A0A0U3EEB4
Organism   Paucibacter sp. KCTC 42545     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3514258..3525394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT984_RS15220 (AT984_15220) - 3515074..3515547 (+) 474 WP_058720820.1 DUF4188 domain-containing protein -
  AT984_RS15225 (AT984_15225) rsmI 3515544..3516449 (-) 906 WP_058720821.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  AT984_RS15230 (AT984_15230) - 3516478..3516879 (+) 402 WP_058720822.1 YraN family protein -
  AT984_RS15235 (AT984_15235) - 3516987..3517559 (+) 573 WP_058720823.1 SIS domain-containing protein -
  AT984_RS15240 (AT984_15240) - 3517645..3518292 (+) 648 WP_058720824.1 BON domain-containing protein -
  AT984_RS15245 (AT984_15245) - 3518347..3519252 (-) 906 WP_058720825.1 NAD(P)-dependent oxidoreductase -
  AT984_RS15250 (AT984_15250) pilU 3519258..3520394 (-) 1137 WP_058720826.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AT984_RS15255 (AT984_15255) pilT 3520549..3521592 (-) 1044 WP_058720827.1 type IV pilus twitching motility protein PilT Machinery gene
  AT984_RS15260 (AT984_15260) - 3521643..3522353 (+) 711 Protein_3032 YggS family pyridoxal phosphate-dependent enzyme -
  AT984_RS15265 (AT984_15265) - 3522499..3523449 (+) 951 WP_058720828.1 IS481 family transposase -
  AT984_RS15270 (AT984_15270) - 3523499..3524245 (+) 747 WP_058720829.1 cytochrome C -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42146.42 Da        Isoelectric Point: 6.6993

>NTDB_id=164104 AT984_RS15250 WP_058720826.1 3519258..3520394(-) (pilU) [Paucibacter sp. KCTC 42545]
MERDQATKFINDLLRLMQSRKGSDLFLTADFPPAVKVDGKVTKVSPQPLTGQHTLQLARAIMNDKQAAEFERNKECNFAI
SPQGIGRFRVNAFLQQGCVGLVLRTIPASLPSISSLELPTVLRDVVQFKRGLVIVVGATGSGKSTTLAAMIDERNETTFG
HIITIEDPVEFVHPHKNCIVSQREVGIDTDSWEMALKNSLRQAPDVILMGELRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLLPRREGKGRVAAVEILLNSPLISDLIFKGEVGEIKEIMKRSREQG
MQTFDQALFDLYELGKVTYEDALRNADSVNDLRLQIKLNSERARSGDIASGTEHFTIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=164104 AT984_RS15250 WP_058720826.1 3519258..3520394(-) (pilU) [Paucibacter sp. KCTC 42545]
ATGGAACGCGACCAAGCCACCAAATTCATCAACGACTTGCTGCGCCTGATGCAGTCGCGCAAGGGTTCGGACTTGTTCCT
CACCGCCGACTTCCCGCCCGCCGTCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTGACCGGTCAGCACA
CCTTGCAGTTGGCCCGCGCCATCATGAACGACAAGCAGGCGGCCGAGTTCGAGCGCAACAAGGAATGCAATTTCGCCATC
TCGCCGCAGGGCATCGGGCGCTTCCGCGTCAATGCTTTCCTGCAGCAAGGTTGCGTGGGCCTGGTGCTGCGGACCATTCC
GGCCAGTCTGCCCAGCATCAGCTCGTTGGAGCTGCCCACCGTGCTGCGCGATGTGGTGCAGTTCAAGCGCGGCCTGGTGA
TCGTGGTCGGCGCCACCGGCTCAGGCAAGAGCACCACGCTGGCGGCCATGATCGATGAACGCAACGAGACCACTTTCGGC
CACATCATCACCATCGAAGACCCGGTCGAGTTTGTGCATCCGCACAAAAACTGCATCGTCAGCCAGCGCGAGGTCGGCAT
CGACACCGACAGCTGGGAGATGGCGCTGAAGAATTCGCTGCGCCAGGCGCCCGATGTGATCTTGATGGGCGAGTTGCGTG
ACCGCGAGACCATGGAACACGCCGTGGCCTTTGCCGAAACCGGCCACCTCTGCATGGCCACCCTGCACGCCAATAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTGCGCAGCTTTTGATGGACTTGTCGCTGAATCT
GCGCGCCCTGGTGTCGCAGCGCCTGCTGCCGCGCCGCGAGGGCAAGGGTCGGGTGGCGGCGGTGGAGATCTTGCTGAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGTGAGGTGGGCGAGATCAAGGAAATCATGAAGCGCTCGCGCGAGCAAGGC
ATGCAGACCTTTGACCAAGCCCTGTTCGATCTGTATGAGCTGGGCAAGGTCACCTACGAAGACGCGCTGCGCAATGCCGA
TTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAATAGCGAGCGCGCGCGTTCCGGCGATATTGCCTCCGGCACCGAAC
ACTTCACCATTCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U3EEB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

56.983

94.709

0.54
  pilU Vibrio cholerae strain A1552

53.994

96.032

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.181

89.153

0.421
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.675

87.831

0.384
  pilT Vibrio cholerae strain A1552

43.675

87.831

0.384
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

44.72

85.185

0.381
  pilT Legionella pneumophila strain Lp02

44.72

85.185

0.381
  pilT Acinetobacter baumannii D1279779

42.643

88.095

0.376
  pilT Acinetobacter baumannii strain A118

42.643

88.095

0.376
  pilT Acinetobacter nosocomialis M2

42.643

88.095

0.376

Multiple sequence alignment