Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NI40_RS18345 Genome accession   NZ_CP012999
Coordinates   3954393..3955373 (-) Length   326 a.a.
NCBI ID   WP_039262967.1    Uniprot ID   -
Organism   Enterobacter sp. E20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3949393..3960373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI40_RS18315 (NI40_018310) - 3950269..3950766 (+) 498 WP_039262968.1 SprT family zinc-dependent metalloprotease -
  NI40_RS18320 (NI40_018315) endA 3950861..3951568 (+) 708 WP_023333343.1 deoxyribonuclease I -
  NI40_RS18325 (NI40_018320) rsmE 3951620..3952351 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NI40_RS18330 (NI40_018325) gshB 3952371..3953318 (+) 948 WP_029739222.1 glutathione synthase -
  NI40_RS18335 (NI40_018330) - 3953406..3953966 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  NI40_RS18340 (NI40_018335) ruvX 3953966..3954382 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  NI40_RS18345 (NI40_018340) pilT 3954393..3955373 (-) 981 WP_039262967.1 type IV pilus twitching motility protein PilT Machinery gene
  NI40_RS18350 (NI40_018345) - 3955391..3956092 (+) 702 WP_039262966.1 YggS family pyridoxal phosphate-dependent enzyme -
  NI40_RS18355 (NI40_018350) - 3956114..3956680 (+) 567 WP_039262965.1 YggT family protein -
  NI40_RS18360 (NI40_018355) yggU 3956677..3956973 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  NI40_RS18365 (NI40_018360) - 3956977..3957570 (+) 594 WP_039262964.1 XTP/dITP diphosphatase -
  NI40_RS18370 (NI40_018365) hemW 3957563..3958705 (+) 1143 WP_039262963.1 radical SAM family heme chaperone HemW -
  NI40_RS18375 (NI40_018370) - 3958769..3959107 (+) 339 WP_039262962.1 endonuclease domain-containing protein -
  NI40_RS18380 (NI40_018375) - 3959190..3959906 (-) 717 WP_033146612.1 DUF2884 domain-containing protein -
  NI40_RS18385 (NI40_018380) - 3959963..3960289 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35470.50 Da        Isoelectric Point: 6.6934

>NTDB_id=159074 NI40_RS18345 WP_039262967.1 3954393..3955373(-) (pilT) [Enterobacter sp. E20]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGCVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=159074 NI40_RS18345 WP_039262967.1 3954393..3955373(-) (pilT) [Enterobacter sp. E20]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCAACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGTAGCCTGCGGGCGGTGCTGGCGCAGAGGCTACTTCC
CGATCTACAGGGCGGGTGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39

Multiple sequence alignment